LOCUS       sequence-363120-       10867 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CAG-hyperCas12a(nuclease active)-HA-P2A-mCherry-T2A-HygroR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10867)
  AUTHORS   Guo LY, Bian J, Davis AE, Liu P, Kempton HR, Zhang X, Chemparathy A,
            Gu B, Lin X, Rane DA, Xu X, Jamiolkowski RM, Hu Y, Wang S, Qi LS
  TITLE     Multiplexed genome regulation in vivo with hyper-efficient Cas12a.
  JOURNAL   Nat Cell Biol. 2022 Apr;24(4):590-600. doi: 
            10.1038/s41556-022-00870-7. Epub 2022 Apr 12.
  PUBMED    35414015
REFERENCE   2  (bases 1 to 10867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-022-00870-7"; journalName: "Nat Cell Biol"; date: 
            "2022-04"; volume: "24"; issue: "4"; pages: "590-600"
COMMENT     Sequence Label: pSLQ10878
FEATURES             Location/Qualifiers
     source          1..10867
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(94..682)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(174..193)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(853..1644,1645..1713))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1476..1495
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1714..1818)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1844..2299)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1981..2002)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2193..2212
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2426..2448
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2440..2457
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2441..2457
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2467..2486
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2467..2485
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   2515..2549
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     intron          3135..4151
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4075..4097
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4159..4178
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             4212..7895
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFRNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIRVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGRGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKRIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
                     YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
                     VKH"
     CDS             7902..7949
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7956..8036
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             8046..8102
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             8103..8810
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(8248..8266)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8516..8535
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             8820..8873
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             8874..9899
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             8874..9899
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(9923..9941)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(9924..9941)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(9961..9978)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9967..10191
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   10407..10469
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(10520..10540)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(10520..10538)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10559..10575)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10559..10575)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10572..10594)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10583..10599
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10607..10613,10614..10631,10632..10637))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10652..10673
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10790..10807)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
       61 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
      121 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
      181 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
      241 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
      301 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
      361 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
      421 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
      481 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
      541 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
      601 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
      661 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
      721 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
      781 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
      841 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
      901 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
      961 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     1021 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     1081 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     1141 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     1201 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     1261 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     1321 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     1381 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     1441 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     1501 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     1561 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     1621 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     1681 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     1741 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     1801 acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat
     1861 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     1921 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     1981 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     2041 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     2101 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     2161 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     2221 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     2281 gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     2341 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     2401 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag
     2461 cgcgcgtaat acgactcact atagggcgaa ttggggcgcg ccattctaga ttaaccctag
     2521 aaagatagtc tgcgtaaaat tgacgcatgc attcttgaaa tattgctctc tctttctaaa
     2581 tagcgcgaat ccgtcgctgt gcatttagga catctcagtc gccgcttgga gctcccgtga
     2641 ggcgtgcttg tcaatgcggt aagtgtcact gattttgaac tataacgacc gcgtgagtca
     2701 aaatgacgca tgattatctt ttacgtgact tttaagattt aactcatacg ataattatat
     2761 tgttatttca tgttctactt acgtgataac ttattatata tatattttct tgttatagat
     2821 atcaactaga atgctagcat gggcccagat ctccatggtc gaggtgagcc ccacgttctg
     2881 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
     2941 attattttgt gcagcgatgg gggcgggggg ggggggggcg cgcgccrggs ggggsggggs
     3001 ggggsgrggg gsggggsggg gsgrggsggr grggkgsggs ggcagccaat cagagcggcg
     3061 cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa
     3121 gcgcgcggcg ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg
     3181 cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg
     3241 acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc
     3301 tgtggctgcg tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct
     3361 cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg
     3421 gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg
     3481 ggagcgcggc cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct
     3541 gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc
     3601 aaccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc
     3661 tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg ggggkggcgg caggkggggg
     3721 tgccgggcgg ggcggggccg ccycgggccg gggrgggctc gggggrgggg cgcggcggcc
     3781 cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat
     3841 cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga
     3901 ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg
     3961 aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc
     4021 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
     4081 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct
     4141 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
     4201 aattcggtac catgagcaag ctggagaagt ttacaaactg ctactccctg tctaagaccc
     4261 tgaggttcaa ggccatccct gtgggcaaga cccaggagaa catcgacaat aagcggctgc
     4321 tggtggagga cgagaagaga gccgaggatt ataagggcgt gaagaagctg ctggatcgct
     4381 actatctgtc ttttatcaac gacgtgctgc acagcatcaa gctgaagaat ctgaacaatt
     4441 acatcagcct gttccggaag aaaaccagaa ccgagaagga gaataaggag ctggagaacc
     4501 tggagatcaa tctgcggaag gagatcgcca aggccttcaa gggcaacgag ggctacaagt
     4561 ccctgtttaa gaaggatatc atcgagacaa tcctgccaga gttcctggac gataaggacg
     4621 agatcgccct ggtgaacagc ttcaatggct ttaccacagc cttcaccggc ttctttcgta
     4681 acagagagaa tatgttttcc gaggaggcca agagcacatc catcgccttc aggtgtatca
     4741 acgagaatct gacccgctac atctctaata tggacatctt cgagaaggtg gacgccatct
     4801 ttgataagca cgaggtgcag gagatcaagg agaagatcct gaacagcgac tatgatgtgg
     4861 aggatttctt tgagggcgag ttctttaact ttgtgctgac acaggagggc atccgcgtgt
     4921 ataacgccat catcggcggc ttcgtgaccg agagcggcga gaagatcaag ggcctgaacg
     4981 agtacatcaa cctgtataat cagaaaacca agcagaagct gcctaagttt aagccactgt
     5041 ataagcaggt gctgagcgat cgggagtctc tgagcttcta cggcaggggc tatacatccg
     5101 atgaggaggt gctggaggtg tttagaaaca ccctgaacaa gaacagcgag atcttcagct
     5161 ccatcaagaa gctggagaag ctgttcaaga attttgacga gtactctagc gccggcatct
     5221 ttgtgaagaa cggccccgcc atcagcacaa tctccaagcg tatcttcggc gagtggaacg
     5281 tgatccggga caagtggaat gccgagtatg acgatatcca cctgaagaag aaggccgtgg
     5341 tgaccgagaa gtacgaggac gatcggagaa agtccttcaa gaagatcggc tccttttctc
     5401 tggagcagct gcaggagtac gccgacgccg atctgtctgt ggtggagaag ctgaaggaga
     5461 tcatcatcca gaaggtggat gagatctaca aggtgtatgg ctcctctgag aagctgttcg
     5521 acgccgattt tgtgctggag aagagcctga agaagaacga cgccgtggtg gccatcatga
     5581 aggacctgct ggattctgtg aagagcttcg agaattacat caaggccttc tttggcgagg
     5641 gcaaggagac aaacagggac gagtccttct atggcgattt tgtgctggcc tacgacatcc
     5701 tgctgaaggt ggaccacatc tacgatgcca tccgcaatta tgtgacccag aagccctact
     5761 ctaaggataa gttcaagctg tattttcaga accctcagtt catgggcggc tgggacaagg
     5821 ataaggagac agactatcgg gccaccatcc tgagatacgg ctccaagtac tatctggcca
     5881 tcatggataa gaagtacgcc aagtgcctgc agaagatcga caaggacgat gtgaacggca
     5941 attacgagaa gatcaactat aagctgctgc ccggccctaa taagatgctg ccaaaggtgt
     6001 tcttttctaa gaagtggatg gcctactata accccagcga ggacatccag aagatctaca
     6061 agaatggcac attcaagaag ggcgatatgt ttaacctgaa tgactgtcac aagctgatcg
     6121 acttctttaa ggatagcatc tcccggtatc caaagtggtc caatgcctac gatttcaact
     6181 tttctgagac agagaagtat aaggacatcg ccggctttta cagagaggtg gaggagcagg
     6241 gctataaggt gagcttcgag tctgccagca agaaggaggt ggataagctg gtggaggagg
     6301 gcaagctgta tatgttccag atctataaca aggacttttc cgataagtct cacggcacac
     6361 ccaatctgca caccatgtac ttcaagctgc tgtttgacga gaacaatcac ggacagatca
     6421 ggctgagcgg aggagcagag ctgttcatga ggcgcgcctc cctgaagaag gaggagctgg
     6481 tggtgcaccc agccaactcc cctatcgcca acaagaatcc agataatccc aagaaaacca
     6541 caaccctgtc ctacgacgtg tataaggata agaggttttc tgaggaccag tacgagctgc
     6601 acatcccaat cgccatcaat aagtgcccca agaacatctt caagatcaat acagaggtgc
     6661 gcgtgctgct gaagcacgac gataacccct atgtgatcgg catcgatagg ggcgagcgca
     6721 atctgctgta tatcgtggtg gtggacggca agggcaacat cgtggagcag tattccctga
     6781 acgagatcat caacaacttc aacggcatca ggatcaagac agattaccac tctctgctgg
     6841 acaagaagga gaaggagagg ttcgaggccc gccagaactg gacctccatc gagaatatca
     6901 aggagctgaa ggccggctat atctctcagg tggtgcacaa gatctgcgag ctggtggaga
     6961 agtacgatgc cgtgatcgcc ctggaggacc tgaactctgg ctttaagaat agccgcgtga
     7021 aggtggagaa gcaggtgtat cagaagttcg agaagatgct gatcgataag ctgaactaca
     7081 tggtggacaa gaagtctaat ccttgtgcaa caggcggcgc cctgaagggc tatcagatca
     7141 ccaataagtt cgagagcttt aagtccatgt ctacccagaa cggcttcatc ttttacatcc
     7201 ctgcctggct gacatccaag atcgatccat ctaccggctt tgtgaacctg ctgaaaacca
     7261 agtataccag catcgccgat tccaagaagt tcatcagctc ctttgacagg atcatgtacg
     7321 tgcccgagga ggatctgttc gagtttgccc tggactataa gaacttctct cgcacagacg
     7381 ccgattacat caagaagtgg aagctgtact cctacggcaa ccggatcaga atcttccgga
     7441 atcctaagaa gaacaacgtg ttcgactggg aggaggtgtg cctgaccagc gcctataagg
     7501 agctgttcaa caagtacggc atcaattatc agcagggcga tatcagagcc ctgctgtgcg
     7561 agcagtccga caaggccttc tactctagct ttatggccct gatgagcctg atgctgcaga
     7621 tgcggaacag catcacaggc cgcaccgacg tggattttct gatcagccct gtgaagaact
     7681 ccgacggcat cttctacgat agccggaact atgaggccca ggagaatgcc atcctgccaa
     7741 agaacgccga cgccaatggc gcctataaca tcgccagaaa ggtgctgtgg gccatcggcc
     7801 agttcaagaa ggccgaggac gagaagctgg ataaggtgaa gatcgccatc tctaacaagg
     7861 agtggctgga gtacgcccag accagcgtga agcacggatc caaaaggccg gcggccacga
     7921 aaaaggccgg ccaggcaaaa aagaaaaagg gatcctaccc atacgatgtt ccagattacg
     7981 cttatcccta cgacgtgcct gattatgcat acccatatga tgtccccgac tatgccggaa
     8041 gcggagctac taacttcagc ctgctgaagc aggctggaga cgtggaggag aaccctggac
     8101 ctatggtgag caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca
     8161 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
     8221 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc
     8281 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc
     8341 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc
     8401 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg
     8461 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg
     8521 taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg
     8581 gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg
     8641 ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg
     8701 tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     8761 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag ggatccggcg
     8821 agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc cctatgaaaa
     8881 agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct
     8941 ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag
     9001 ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg
     9061 tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat
     9121 tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc
     9181 tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg
     9241 ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc
     9301 aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc
     9361 aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc
     9421 tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca
     9481 atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg
     9541 gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg
     9601 agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc
     9661 gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt
     9721 tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga
     9781 ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag
     9841 aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaatagc
     9901 tcgagtcgag agagggccct attctatagt gtcacctaaa tgctagagct cgctgatcag
     9961 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
    10021 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
    10081 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
    10141 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
    10201 tcgaggcggc cgctgatcac aattgtctag ttaaaagttt tgttacttta tagaagaaat
    10261 tttgagtttt tgtttttttt taataaataa ataaacataa ataaattgtt tgttgaattt
    10321 attattagta tgtaagtgta aatataataa aacttaatat ctattcaaat taataaataa
    10381 acctcgatat acagaccgat aaaacacatg cgtcaatttt acgcatgatt atctttaacg
    10441 tacgtcacaa tatgattatc tttctagggt taatctagta tacgcgtatg cggccgctta
    10501 attaatccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt
    10561 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
    10621 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
    10681 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
    10741 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
    10801 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
    10861 tacggtt
//
