LOCUS       sequence-363124-       10465 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of a lysosome-residential PEN2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10465)
  AUTHORS   Ma T, Tian X, Zhang B, Li M, Wang Y, Yang C, Wu J, Wei X, Qu Q, Yu 
            Y, Long S, Feng JW, Li C, Zhang C, Xie C, Wu Y, Xu Z, Chen J, Yu Y, 
            Huang X, He Y, Yao L, Zhang L, Zhu M, Wang W, Wang ZC, Zhang M, Bao 
            Y, Jia W, Lin SY, Ye Z, Piao HL, Deng X, Zhang CS, Lin SC
  TITLE     Low-dose metformin targets the lysosomal AMPK pathway through PEN2.
  JOURNAL   Nature. 2022 Mar;603(7899):159-165. doi: 10.1038/s41586-022-04431-8.
            Epub 2022 Feb 23.
  PUBMED    35197629
REFERENCE   2  (bases 1 to 10465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-022-04431-8"; journalName: "Nature"; date: 
            "2022-03"; volume: "603"; issue: "7899"; pages: "159-165"
COMMENT     Sequence Label: pBoBi-hLAMP2-C-PEN2
FEATURES             Location/Qualifiers
     source          1..10465
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             149..329
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    376..501
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    994..1227
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1412..1456
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1708..1824
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1847..2150
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2151..2354
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2351..2375
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2453..2479
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2486..2512
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2516..2542
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     intron          4183..4412
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     4395..4414
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    4589..5177
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4642..4662)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5060..5071)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5584..5764
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5790..5807)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5796..6020
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6066..6494
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6153..6172)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6363..6384
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6503..6523)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6508..6837
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6688..6823
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6750..6769
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6885..6932
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6951..7325
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7455..7576
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7492..7511)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7546..7565
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7625..7641)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7625..7641)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7638..7660)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7649..7665
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7673..7679,7680..7697,7698..7703))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7718..7739
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7856..7873)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8027..8615)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8107..8126)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8786..9577,9578..9646))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9409..9428
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9647..9751)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9826..9845)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10017..10396
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        join(10398..10465,1..131)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
       61 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
      121 tatataagca gcgcgttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg
      181 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
      241 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
      301 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
      361 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
      421 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
      481 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
      541 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
      601 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
      661 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
      721 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
      781 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
      841 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
      901 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
      961 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     1021 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     1081 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     1141 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     1201 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     1261 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     1321 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     1381 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     1441 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     1501 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     1561 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     1621 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
     1681 atccattcga ttagtgaacg gatcaacttt taaaagaaaa ggggggattg gggggtacag
     1741 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     1801 acaaattaca aaattcaaaa ttttatcgat aagcttggga gttccgcgtt acataactta
     1861 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     1921 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     1981 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     2041 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     2101 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
     2161 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     2221 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     2281 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     2341 atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt
     2401 ttgacctcca tagaagacac cgactgagct ccttaaggtt aacgccacca tgtaccctta
     2461 tgacgtacct gactatgctg gcgcctaccc ttatgacgta cctgactatg ctggataccc
     2521 ttatgacgta cctgactatg ctggatctag agaattcgga tccatggtgt gcttccgcct
     2581 cttcccggtt ccgggctcag ggctcgttct ggtctgccta gtcctgggag ctgtgcggtc
     2641 ttatgcattg gaacttaatt tgacagattc agaaaatgcc acttgccttt atgcaaaatg
     2701 gcagatgaat ttcacagtac gctatgaaac tacaaataaa acttataaaa ctgtaaccat
     2761 ttcagaccat ggcactgtga catataatgg aagcatttgt ggggatgatc agaatggtcc
     2821 caaaatagca gtgcagttcg gacctggctt ttcctggatt gcgaatttta ccaaggcagc
     2881 atctacttat tcaattgaca gcgtctcatt ttcctacaac actggtgata acacaacatt
     2941 tcctgatgct gaagataaag gaattcttac tgttgatgaa cttttggcca tcagaattcc
     3001 attgaatgac ctttttagat gcaatagttt atcaactttg gaaaagaatg atgttgtcca
     3061 acactactgg gatgttcttg tacaagcttt tgtccaaaat ggcacagtga gcacaaatga
     3121 gttcctgtgt gataaagaca aaacttcaac agtggcaccc accatacaca ccactgtgcc
     3181 atctcctact acaacaccta ctccaaagga aaaaccagaa gctggaacct attcagttaa
     3241 taatggcaat gatacttgtc tgctggctac catggggctg cagctgaaca tcactcagga
     3301 taaggttgct tcagttatta acatcaaccc caatacaact cactccacag gcagctgccg
     3361 ttctcacact gctctactta gactcaatag cagcaccatt aagtatctag actttgtctt
     3421 tgctgtgaaa aatgaaaacc gattttatct gaaggaagtg aacatcagca tgtatttggt
     3481 taatggctcc gttttcagca ttgcaaataa caatctcagc tactgggatg cccccctggg
     3541 aagttcttat atgtgcaaca aagagcagac tgtttcagtg tctggagcat ttcagataaa
     3601 tacctttgat ctaagggttc agcctttcaa tgtgacacaa ggaaagtatt ctacagccca
     3661 agagtgttcg ctggatgatg acaccattct aatcccaatt atagttggtg ctggtctttc
     3721 aggcttgatt atcgttatag tgattgctta cgtaattggc agaagaaaaa gttatgctgg
     3781 atatcagact ctgatgaacc tggagcgagt gtccaatgag gagaaattga acctgtgccg
     3841 gaagtactac ctgggggggt ttgctttcct gccttttctc tggttggtca acatcttctg
     3901 gttcttccga gaggccttcc ttgtcccagc ctacacagaa cagagccaaa tcaaaggcta
     3961 tgtctggcgc tcagctgtgg gcttcctctt ctgggtgata gtgctcacct cctggatcac
     4021 catcttccag atctaccggc cccgctgggg tgcccttggg gactacctct ccttcaccat
     4081 acccctgggc acccccctcg agccatggaa gcttgatatc taactgactg aaccggtggt
     4141 accgaggaat taattcgctg tctgcgaggg ccagctgttg gggtgagtac tccctctcaa
     4201 aagcgggcat gacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat
     4261 tcacctggcc cgcggtgatg cctttgaggg tggccgcgtc catctggtca gaaaagacaa
     4321 tctttttgtt gtcaagcttg aggtgtggca ggcttgagat ctggccatac acttgagtga
     4381 caatgacatc cactttgcct ttctctccac aggtgtccac tcccaggtcc aactgcaggt
     4441 cgagcatgca tctagggcgg ccaattccgc cgatcctgga aaaacatgga gcaatcacaa
     4501 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg
     4561 aggtgggttt tccagtcaca cctcagacaa tcaacctctg gattacaaaa tttgtgaaag
     4621 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     4681 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     4741 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     4801 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     4861 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     4921 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     4981 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     5041 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     5101 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     5161 ttgggccgcc tccccgcctg gaattcgagc tcggtacctt taagaccaat gacttacaag
     5221 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     5281 tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
     5341 gattggcaga actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc
     5401 tacaagctag taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc
     5461 cgcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag
     5521 tggaggtttg acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt
     5581 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     5641 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     5701 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     5761 agcagggccg ctttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     5821 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     5881 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     5941 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     6001 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     6061 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     6121 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     6181 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     6241 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     6301 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     6361 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     6421 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     6481 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     6541 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     6601 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     6661 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     6721 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     6781 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     6841 ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat
     6901 cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt
     6961 tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga
     7021 ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg
     7081 acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg
     7141 cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca
     7201 cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc
     7261 gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg
     7321 actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     7381 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     7441 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     7501 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     7561 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
     7621 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     7681 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     7741 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     7801 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     7861 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     7921 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     7981 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     8041 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     8101 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     8161 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     8221 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     8281 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     8341 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     8401 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     8461 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     8521 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     8581 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     8641 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     8701 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     8761 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     8821 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     8881 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     8941 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     9001 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     9061 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     9121 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     9181 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     9241 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     9301 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     9361 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     9421 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     9481 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     9541 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     9601 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     9661 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     9721 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     9781 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
     9841 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
     9901 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
     9961 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
    10021 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
    10081 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
    10141 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
    10201 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
    10261 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
    10321 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
    10381 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
    10441 tgactcacgg ggatttccaa gtctc
//
