LOCUS       sequence-363129-       10342 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of a mutant of ATP6AP1 with its 
            PEN2-interacting domain replaced with the transmembrane (TM) domain 
            of LAMP2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10342)
  AUTHORS   Ma T, Tian X, Zhang B, Li M, Wang Y, Yang C, Wu J, Wei X, Qu Q, Yu 
            Y, Long S, Feng JW, Li C, Zhang C, Xie C, Wu Y, Xu Z, Chen J, Yu Y, 
            Huang X, He Y, Yao L, Zhang L, Zhu M, Wang W, Wang ZC, Zhang M, Bao 
            Y, Jia W, Lin SY, Ye Z, Piao HL, Deng X, Zhang CS, Lin SC
  TITLE     Low-dose metformin targets the lysosomal AMPK pathway through PEN2.
  JOURNAL   Nature. 2022 Mar;603(7899):159-165. doi: 10.1038/s41586-022-04431-8.
            Epub 2022 Feb 23.
  PUBMED    35197629
REFERENCE   2  (bases 1 to 10342)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-022-04431-8"; journalName: "Nature"; date: 
            "2022-03"; volume: "603"; issue: "7899"; pages: "159-165"
COMMENT     Sequence Label: pBoBi-hAP1-C-LAMP2 TM
FEATURES             Location/Qualifiers
     source          1..10342
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             823..849
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             856..882
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             886..912
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     intron          984..1213
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     1196..1215
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    1390..1978
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1443..1463)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1861..1872)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2385..2565
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2591..2608)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2597..2821
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2867..3295
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2954..2973)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3164..3185
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3304..3324)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3309..3638
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3489..3624
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3551..3570
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        3686..3733
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3752..4126
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4256..4377
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4293..4312)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4347..4366
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4426..4442)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4426..4442)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4439..4461)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4450..4466
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4474..4480,4481..4498,4499..4504))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4519..4540
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4657..4674)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4828..5416)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4908..4927)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5587..6378,6379..6447))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6210..6229
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6448..6552)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6627..6646)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6818..7197
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7199..7397
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             7415..7595
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7642..7767
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8260..8493
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8678..8722
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8974..9090
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        9113..9416
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9417..9620
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9617..9641
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gtcctgagca cactcaagtc cgaagatgtc ccatacacag cggccctcac agcggtccgc
       61 ccttccaggg tggcccgtga tgtagccgtg gtggccggag ggctaggtcg ccagctgcta
      121 caaaaacagc cagtatcacc tgtgatccat cctcctgtga gttacaatga caccgctccc
      181 cggatcctgt tctgggccca aaacttctct gtggcgtaca aggaccagtg ggaggacctg
      241 actcccctca cctttggggt gcaggaactc aacctgactg gctccttctg gaatgactcc
      301 tttgccaggc tctcactgac ctatgaacga ctctttggta ccacagtgac attcaagttc
      361 attctggcca accgcctcta cccagtgtct gcccggcact ggtttaccat ggagcgcctc
      421 gaagtccaca gcaatggctc cgtcgcctac ttcaatgctt cccaggtcac agggcccagc
      481 atctactcct tccactgcga gtatgtcagc agcctgagca agaagggtag tctcctcgtg
      541 gcccgcacgc agccctctcc ctggcagatg atgcttcagg acttccagat ccaggctttc
      601 aacgtaatgg gggagcagtt ctcctacgcc agcgactgtg cccttgtgcc catagcggtg
      661 ggagctgcct tggcaggagt acttattcta gtgttgctgg cttattttat tggtacctat
      721 ggcctgcaca tgatcctcag cctcaagacc atggatcgct ttgatgacca caagggcccc
      781 actatttctt tgacccagat tgtgctcgag ccatggaagc tttaccctta tgacgtacct
      841 gactatgctg gcgcctaccc ttatgacgta cctgactatg ctggataccc ttatgacgta
      901 cctgactatg ctggagatat ctaactgact gaaccggtgg taccgaggaa ttaattcgct
      961 gtctgcgagg gccagctgtt ggggtgagta ctccctctca aaagcgggca tgacttctgc
     1021 gctaagattg tcagtttcca aaaacgagga ggatttgata ttcacctggc ccgcggtgat
     1081 gcctttgagg gtggccgcgt ccatctggtc agaaaagaca atctttttgt tgtcaagctt
     1141 gaggtgtggc aggcttgaga tctggccata cacttgagtg acaatgacat ccactttgcc
     1201 tttctctcca caggtgtcca ctcccaggtc caactgcagg tcgagcatgc atctagggcg
     1261 gccaattccg ccgatcctgg aaaaacatgg agcaatcaca agtagcaata cagcagctac
     1321 caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac
     1381 acctcagaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     1441 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     1501 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     1561 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     1621 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     1681 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     1741 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     1801 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     1861 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     1921 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     1981 ggaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     2041 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata
     2101 tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag aactacacac
     2161 cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     2221 agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga
     2281 gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc
     2341 tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag
     2401 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     2461 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     2521 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagggcc gctttaaacc
     2581 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     2641 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     2701 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     2761 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     2821 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
     2881 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     2941 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     3001 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     3061 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     3121 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     3181 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     3241 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
     3301 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     3361 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     3421 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     3481 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     3541 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     3601 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     3661 ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat
     3721 agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt
     3781 gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc
     3841 ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat
     3901 cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg
     3961 cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc
     4021 cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga
     4081 cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga
     4141 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     4201 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     4261 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     4321 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     4381 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     4441 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     4501 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     4561 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     4621 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     4681 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     4741 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     4801 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     4861 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     4921 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     4981 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     5041 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     5101 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     5161 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     5221 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     5281 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     5341 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     5401 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     5461 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     5521 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     5581 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     5641 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     5701 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     5761 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     5821 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     5881 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     5941 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     6001 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     6061 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     6121 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     6181 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     6241 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     6301 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     6361 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     6421 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     6481 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     6541 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct
     6601 cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     6661 tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct
     6721 acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt
     6781 gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat
     6841 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     6901 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     6961 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     7021 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     7081 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     7141 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     7201 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     7261 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     7321 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     7381 gaggtctata taagcagcgc gttttgcctg tactgggtct ctctggttag accagatctg
     7441 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     7501 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     7561 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
     7621 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
     7681 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
     7741 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
     7801 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
     7861 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     7921 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     7981 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     8041 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     8101 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     8161 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     8221 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     8281 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     8341 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     8401 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     8461 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     8521 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     8581 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     8641 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     8701 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     8761 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     8821 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     8881 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     8941 agacagatcc attcgattag tgaacggatc aacttttaaa agaaaagggg ggattggggg
     9001 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     9061 acaaaaacaa attacaaaat tcaaaatttt atcgataagc ttgggagttc cgcgttacat
     9121 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     9181 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     9241 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
     9301 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     9361 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
     9421 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
     9481 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
     9541 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
     9601 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac
     9661 gctgttttga cctccataga agacaccgac tgagctcctt aaggttaact ctagagaatt
     9721 cggatccatg atggcggcca tggcgacggc tcgagtgcgg atggggccgc ggtgcgccca
     9781 ggcgctctgg cgcatgccgt ggctgccggt gtttttgtcg ttggcggcgg cggcggcggc
     9841 ggcagcggcg gagcagcagg tcccgctggt gctgtggtcg agtgaccggg acttgtgggc
     9901 tcctgcggcc gacactcatg aaggccacat caccagcgac ttgcagctct ctacctactt
     9961 agatcccgcc ctggagctgg gtcccaggaa tgtgctgctg ttcctgcagg acaagctgag
    10021 cattgaggat ttcacagcat atggcggtgt gtttggaaac aagcaggaca gcgccttttc
    10081 taacctagag aatgccctgg acctggcccc ctcctcactg gtgcttcctg ccgtcgactg
    10141 gtatgcagtc agcactctga ccacttacct gcaggagaag ctcggggcca gccccttgca
    10201 tgtggacctg gccaccctgc gggagctgaa gctcaatgcc agcctccctg ctctgctgct
    10261 cattcgcctg ccctacacag ccagctctgg tctgatggca cccagggaag tcctcacagg
    10321 caacgatgag gtcatcgggc ag
//
