LOCUS       sequence-363135-        9235 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of a PEN2 mutant losing its binding affinity 
            to metformin at the C-terminus (lysosome lumen)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9235)
  AUTHORS   Ma T, Tian X, Zhang B, Li M, Wang Y, Yang C, Wu J, Wei X, Qu Q, Yu 
            Y, Long S, Feng JW, Li C, Zhang C, Xie C, Wu Y, Xu Z, Chen J, Yu Y, 
            Huang X, He Y, Yao L, Zhang L, Zhu M, Wang W, Wang ZC, Zhang M, Bao 
            Y, Jia W, Lin SY, Ye Z, Piao HL, Deng X, Zhang CS, Lin SC
  TITLE     Low-dose metformin targets the lysosomal AMPK pathway through PEN2.
  JOURNAL   Nature. 2022 Mar;603(7899):159-165. doi: 10.1038/s41586-022-04431-8.
            Epub 2022 Feb 23.
  PUBMED    35197629
REFERENCE   2  (bases 1 to 9235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-022-04431-8"; journalName: "Nature"; date: 
            "2022-03"; volume: "603"; issue: "7899"; pages: "159-165"
COMMENT     Sequence Label: pBoBi-hPEN2 D90A
FEATURES             Location/Qualifiers
     source          1..9235
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(131..151)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        136..465
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      316..451
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     378..397
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        513..560
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             579..953
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1083..1204
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1120..1139)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1174..1193
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1253..1269)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1253..1269)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1266..1288)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1277..1293
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1301..1307,1308..1325,1326..1331))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1346..1367
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1484..1501)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1655..2243)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1735..1754)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2414..3205,3206..3274))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3037..3056
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3275..3379)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3454..3473)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3645..4024
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4026..4224
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             4242..4422
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4469..4594
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5087..5320
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5505..5549
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5801..5917
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        5940..6243
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6244..6447
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6444..6468
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             6546..6572
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6579..6605
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6609..6635
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     intron          7046..7275
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     7258..7277
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    7452..8040
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7505..7525)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7923..7934)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8447..8627
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8653..8670)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8659..8883
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(8929..9235,1..122)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9016..9035)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(9226..9235,1..12)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
       61 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
      121 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
      181 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
      241 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
      301 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
      361 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
      421 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
      481 tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata
      541 tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg accagtgccg
      601 ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg
      661 ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc
      721 tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg
      781 tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg
      841 acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc
      901 tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc
      961 tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     1021 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     1081 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     1141 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     1201 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     1261 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     1321 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     1381 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     1441 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     1501 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     1561 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     1621 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     1681 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     1741 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     1801 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     1861 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     1921 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     1981 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     2041 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     2101 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     2161 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     2221 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     2281 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     2341 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     2401 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     2461 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     2521 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     2581 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     2641 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     2701 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     2761 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     2821 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     2881 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     2941 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     3001 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     3061 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     3121 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     3181 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     3241 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     3301 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     3361 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
     3421 gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     3481 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat
     3541 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
     3601 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac
     3661 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
     3721 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     3781 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     3841 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     3901 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     3961 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     4021 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
     4081 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     4141 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     4201 acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc
     4261 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     4321 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     4381 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
     4441 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
     4501 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     4561 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     4621 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
     4681 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     4741 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     4801 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     4861 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     4921 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     4981 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     5041 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     5101 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     5161 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     5221 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     5281 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     5341 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     5401 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     5461 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     5521 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     5581 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     5641 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     5701 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     5761 gagacagaga cagatccatt cgattagtga acggatcaac ttttaaaaga aaagggggga
     5821 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     5881 aagaattaca aaaacaaatt acaaaattca aaattttatc gataagcttg ggagttccgc
     5941 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     6001 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     6061 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     6121 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     6181 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     6241 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     6301 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     6361 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     6421 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
     6481 catccacgct gttttgacct ccatagaaga caccgactga gctccttaag gttaacgcca
     6541 ccatgtaccc ttatgacgta cctgactatg ctggcgccta cccttatgac gtacctgact
     6601 atgctggata cccttatgac gtacctgact atgctggatc tagagaattc ggatccatga
     6661 acctggagcg agtgtccaat gaggagaaat tgaacctgtg ccggaagtac tacctggggg
     6721 ggtttgcttt cctgcctttt ctctggttgg tcaacatctt ctggttcttc cgagaggcct
     6781 tccttgtccc agcctacaca gaacagagcc aaatcaaagg ctatgtctgg cgctcagctg
     6841 tgggcttcct cttctgggtg atagtgctca cctcctggat caccatcttc cagatctacc
     6901 ggccccgctg gggtgccctt ggggcctacc tctccttcac catacccctg ggcacccccc
     6961 tcgagccatg gaagcttgat atctaactga ctgaaccggt ggtaccgagg aattaattcg
     7021 ctgtctgcga gggccagctg ttggggtgag tactccctct caaaagcggg catgacttct
     7081 gcgctaagat tgtcagtttc caaaaacgag gaggatttga tattcacctg gcccgcggtg
     7141 atgcctttga gggtggccgc gtccatctgg tcagaaaaga caatcttttt gttgtcaagc
     7201 ttgaggtgtg gcaggcttga gatctggcca tacacttgag tgacaatgac atccactttg
     7261 cctttctctc cacaggtgtc cactcccagg tccaactgca ggtcgagcat gcatctaggg
     7321 cggccaattc cgccgatcct ggaaaaacat ggagcaatca caagtagcaa tacagcagct
     7381 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc
     7441 acacctcaga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     7501 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     7561 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     7621 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     7681 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     7741 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     7801 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
     7861 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     7921 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     7981 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     8041 ctggaattcg agctcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     8101 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     8161 tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc agaactacac
     8221 accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
     8281 tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt
     8341 gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg
     8401 cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt
     8461 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     8521 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     8581 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg ccgctttaaa
     8641 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     8701 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     8761 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     8821 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     8881 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
     8941 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     9001 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     9061 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     9121 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     9181 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctctt
//
