LOCUS       sequence-363142-       10312 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of a mitochondrion-residential PEN2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10312)
  AUTHORS   Ma T, Tian X, Zhang B, Li M, Wang Y, Yang C, Wu J, Wei X, Qu Q, Yu 
            Y, Long S, Feng JW, Li C, Zhang C, Xie C, Wu Y, Xu Z, Chen J, Yu Y, 
            Huang X, He Y, Yao L, Zhang L, Zhu M, Wang W, Wang ZC, Zhang M, Bao 
            Y, Jia W, Lin SY, Ye Z, Piao HL, Deng X, Zhang CS, Lin SC
  TITLE     Low-dose metformin targets the lysosomal AMPK pathway through PEN2.
  JOURNAL   Nature. 2022 Mar;603(7899):159-165. doi: 10.1038/s41586-022-04431-8.
            Epub 2022 Feb 23.
  PUBMED    35197629
REFERENCE   2  (bases 1 to 10312)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-022-04431-8"; journalName: "Nature"; date: 
            "2022-03"; volume: "603"; issue: "7899"; pages: "159-165"
COMMENT     Sequence Label: pBoBi-hTOMM20-C-PEN2
FEATURES             Location/Qualifiers
     source          1..10312
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        311..509
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             527..707
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    754..879
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1372..1605
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1790..1834
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2086..2202
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2225..2528
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2529..2732
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2729..2753
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3287..3313
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3320..3346
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3350..3376
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     intron          3787..4016
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     3999..4018
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    4193..4781
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4246..4266)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4664..4675)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5188..5368
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5394..5411)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5400..5624
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5670..6098
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5757..5776)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5967..5988
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6107..6127)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6112..6441
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6292..6427
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6354..6373
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6489..6536
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6555..6929
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7059..7180
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7096..7115)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7150..7169
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7229..7245)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7229..7245)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7242..7264)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7253..7269
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7277..7283,7284..7301,7302..7307))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7322..7343
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7460..7477)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7631..8219)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7711..7730)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8390..9181,9182..9250))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9013..9032
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9251..9355)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9430..9449)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9621..10000
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
       61 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
      121 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagakmaw
      181 wtrmcaakta crcmmsctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      241 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      301 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      361 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      421 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      481 cgtgtacggt gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt
      541 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
      601 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
      661 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
      721 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
      781 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
      841 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
      901 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
      961 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     1021 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     1081 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     1141 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     1201 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     1261 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     1321 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     1381 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     1441 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     1501 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     1561 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     1621 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     1681 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     1741 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     1801 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     1861 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     1921 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     1981 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
     2041 agagagagac agagacagat ccattcgatt agtgaacgga tcaactttta aaagaaaagg
     2101 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2161 aactaaagaa ttacaaaaac aaattacaaa attcaaaatt ttatcgataa gcttgggagt
     2221 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     2281 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     2341 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     2401 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     2461 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     2521 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
     2581 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
     2641 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
     2701 gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga
     2761 gacgccatcc acgctgtttt gacctccata gaagacaccg actgagctcc ttaaggttaa
     2821 ctctagagaa ttcggatcca tggtgggtcg gaacagcgcc atcgccgccg gtgtatgcgg
     2881 ggcccttttc attgggtact gcatctactt cgaccgcaaa agacgaagtg accccaactt
     2941 caagaacagg cttcgagaac gaagaaagaa acagaagctt gccaaggaga gagctgggct
     3001 ttccaagtta cctgacctta aagatgctga agctgttcag aagttcttcc ttgaagaaat
     3061 acagcttggt gaagagttac tagctcaagg tgaatatgag aagggcgtag accatctgac
     3121 aaatgcaatt gctgtgtgtg gacagccaca gcagttactg caggtcttac agcaaactct
     3181 tccaccacca gtgttccaga tgcttctgac taagctccca acaattagtc agagaattgt
     3241 aagtgctcag agcttggctg aagatgatgt ggaaaacgcc accatgtacc cttatgacgt
     3301 acctgactat gctggcgcct acccttatga cgtacctgac tatgctggat acccttatga
     3361 cgtacctgac tatgctggat ctagagaatt cggatccatg aacctggagc gagtgtccaa
     3421 tgaggagaaa ttgaacctgt gccggaagta ctacctgggg gggtttgctt tcctgccttt
     3481 tctctggttg gtcaacatct tctggttctt ccgagaggcc ttccttgtcc cagcctacac
     3541 agaacagagc caaatcaaag gctatgtctg gcgctcagct gtgggcttcc tcttctgggt
     3601 gatagtgctc acctcctgga tcaccatctt ccagatctac cggccccgct ggggtgccct
     3661 tggggactac ctctccttca ccatacccct gggcaccccc ctcgagccat ggaagcttga
     3721 tatctaactg actgaaccgg tggtaccgag gaattaattc gctgtctgcg agggccagct
     3781 gttggggtga gtactccctc tcaaaagcgg gcatgacttc tgcgctaaga ttgtcagttt
     3841 ccaaaaacga ggaggatttg atattcacct ggcccgcggt gatgcctttg agggtggccg
     3901 cgtccatctg gtcagaaaag acaatctttt tgttgtcaag cttgaggtgt ggcaggcttg
     3961 agatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     4021 ccactcccag gtccaactgc aggtcgagca tgcatctagg gcggccaatt ccgccgatcc
     4081 tggaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct
     4141 ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag acaatcaacc
     4201 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     4261 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     4321 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     4381 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     4441 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     4501 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     4561 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
     4621 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     4681 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     4741 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaattc gagctcggta
     4801 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     4861 gggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc
     4921 taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc agggatcaga
     4981 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa
     5041 gaagccaatg aaggagagaa cacccgcttg ttacaccctg tgagcctgca tgggatggat
     5101 gacccggaga gagaagtatt agagtggagg tttgacagcc gcctagcatt tcatcacatg
     5161 gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg
     5221 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     5281 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     5341 ttttagtcag tgtggaaaat ctctagcagg gccgctttaa acccgctgat cagcctcgac
     5401 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     5461 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     5521 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     5581 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     5641 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     5701 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     5761 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     5821 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     5881 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     5941 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     6001 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     6061 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
     6121 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     6181 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     6241 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     6301 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     6361 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     6421 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     6481 cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg
     6541 tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg
     6601 tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg
     6661 acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc
     6721 aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg
     6781 ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg
     6841 agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg
     6901 tgcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg
     6961 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     7021 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     7081 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     7141 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     7201 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     7261 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     7321 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     7381 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     7441 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     7501 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     7561 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     7621 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     7681 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     7741 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     7801 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     7861 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     7921 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     7981 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     8041 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     8101 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     8161 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     8221 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     8281 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     8341 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     8401 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     8461 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     8521 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     8581 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     8641 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     8701 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     8761 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     8821 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     8881 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     8941 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     9001 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     9061 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     9121 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     9181 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     9241 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     9301 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     9361 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg
     9421 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg
     9481 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt
     9541 gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt
     9601 acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac
     9661 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     9721 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     9781 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     9841 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     9901 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     9961 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
    10021 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctct gggcataatg
    10081 ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac
    10141 ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa
    10201 agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc
    10261 gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga ac
//
