LOCUS       sequence-363152-        9468 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bxb1-specific donor plasmid for intergrating a transcription unit 
            for expression of jRCaMP1b calcium reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9468)
  AUTHORS   Blanch-Asensio A, Grandela C, Brandao KO, de Korte T, Mei H, 
            Ariyurek Y, Yiangou L, Mol MPH, van Meer BJ, Kloet SL, Mummery CL, 
            Davis RP
  TITLE     STRAIGHT-IN enables high-throughput targeting of large DNA payloads 
            in human pluripotent stem cells.
  JOURNAL   Cell Rep Methods. 2022 Sep 22;2(10):100300. doi: 
            10.1016/j.crmeth.2022.100300. eCollection 2022 Oct 24.
  PUBMED    36313798
REFERENCE   2  (bases 1 to 9468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Methods."; date: "2022-09-22"; volume: "2(10):100300. doi"; pages: "
            10.1016/j.crmeth.2022.100300. eCollection 2022 Oct 24"
COMMENT     Sequence Label: Bxb1_jRCaMP1b
FEATURES             Location/Qualifiers
     source          1..9468
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    51..75
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        151..1329
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          382..1320
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1277..1297
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1337..1346
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    1349..1386
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     protein_bind    complement(1395..1428)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        1651..1954
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          2232..3248
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3172..3194
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3256..3275
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3382..3399
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3403..3435
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(3439..3447,3448..3462)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(4787..4806)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4852..4907
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4906..4925)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     protein_bind    complement(5295..5327)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     complement(5335..5352)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5335..5351)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    5388..5412
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      5500..6210
                     /label=oriV
                     /note="incP origin of replication"
     promoter        6231..6335
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6336..7130
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      7301..7889
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7790..7809
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             8225..9373
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
ORIGIN
        1 agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg tggcaggata
       61 tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg gacgttttta
      121 atgtactggg gttgaacact ctgtgccatt ggctccggtg cccgtcagtg ggcagagcgc
      181 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag
      241 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
      301 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
      361 gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt
      421 acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta cgtgattctt
      481 gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc
      541 ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc
      601 tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt
      661 tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat
      721 ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag
      781 cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag
      841 tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc
      901 tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc
      961 ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg ggcgggtgag
     1021 tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg
     1081 gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag tacgtcgtct
     1141 ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact
     1201 gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt
     1261 ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag
     1321 gtgtcgtgag gaattcgcca ccatggccgg cttgtcgacg acggcggtct ccgtcgtcag
     1381 gatcatccgg atccataact tcgtatagca tacattatac gaagttatca tgatattcgg
     1441 caagcaggca tcgtagctgc cgatgtatca cctctcgcaa gaattcaagc ttggaggccg
     1501 cgaattctga aagaccccac ctgtaggttt ggcaagccca gggatgtacg tccctaaccc
     1561 gctagggggc agcaactagt cccaggcctg cactgccgcc tgccggcagg ggtccagtcg
     1621 ctagcgcatg cctgcactac tggagttccg cgttacataa cttacggtaa atggcccgcc
     1681 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     1741 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     1801 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     1861 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     1921 gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt
     1981 cactctcccc atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt
     2041 attttgtgca gcgatggggg cggggggggg gggggcgcgc gccrggsggg gsggggsggg
     2101 gsgrggggsg gggsggggsg rggsggrgrg gkgsggsggc rgccaatcag agcggcgcgc
     2161 tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg
     2221 cgcggcgggc gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct
     2281 cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg
     2341 gcccttctcc tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt
     2401 ggctgcgtga aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg
     2461 ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg
     2521 gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga
     2581 gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg
     2641 tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac
     2701 cccccctgca cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc
     2761 gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg gkggcggcag gkgggggtgc
     2821 cgggcggggc ggggccgccy cgggccgggg rgggctcggg ggrggggcgc ggcggccccc
     2881 ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt
     2941 gcgagagggc gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc
     3001 gccgccgcac cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa
     3061 tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc
     3121 ggggctgtcc gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct
     3181 tctggcgtgt gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt
     3241 tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat
     3301 tccgatcgcg ccaccatgct gcagaacgag cttgctctta agttggctgg acttgatatt
     3361 aacaagactg gaggaggttc tcatcatcat catcatcatg gtatggctag catgactggt
     3421 ggacagcaaa tgggtcggga tctgtacgac gatgacgata aggatctcgc aacaatggtc
     3481 gactcatcgc gacgtaagtg gaataagtgg ggtcacgcag tcagagctat aggtcggctg
     3541 agctcagcga acaacaccga aatgatgtac ccagcggatg gtggtctgcg tggttacact
     3601 cacatggcgc tgaaagttga tggcggcggt cacctgtcct gttctttcgt gaccacctac
     3661 cgctccaaaa agactgtcgg caacattaag atgcctgcca ttcattacgt cagccaccgt
     3721 ctggagcgcc tggaggagag cgataacgaa atgtttgtcg tacagcgtga acacgcagtt
     3781 gccaagtttg tgggcctggg tggtggcggc ggtaccggag ggagcatgaa ctccctgatc
     3841 aaggagaaca tgcgtatgaa agtggttctg gaaggctccg taaacggcca ccagttcaaa
     3901 tgcactggtg aaggcgaagg caacccgtat atgggcaccc agactatgcg tatcaaagtg
     3961 atcgagggtg gtccgctgcc gtttgcgttc gacatcctgg cgacgtcctt tatgtatggc
     4021 tcccgtacct tcatcaaata tccgaaaggc atcccggatt tctttaagca gtccttcccg
     4081 gaaggtttta cctgggaacg tgtgacccgt tacgaggacg gcggcgtaat taccgttatg
     4141 caagacacgt ctctggagga tggctgcctg gtgtatcacg tgcaggttcg cggtgtgaac
     4201 ttcccgagca atggtgctgt aatgcaaaag aaaaccaaag gttgggagcc tacggactcc
     4261 caactgactg aagagcagat cgcagaattt aaagaggctt tctccctatt tgacaaggac
     4321 ggggatggga caataacaac caaggagatg gggacggtga tgcggtccct ggggcagaac
     4381 cccacagaag cagagctgca ggacatgatc aatgaagtag atgccgacgg tgacggcaca
     4441 atcgacttcc ctgagttcct gattatgatg gcaggcaaaa tgaaatacac agacagtgaa
     4501 gaagaaatta gagaagcgtt cggcgtgttt gataaggatg gcaatggcta catcagtgca
     4561 gcagagcttc gccacgtgat gacaaacctt ggagagaagt taacagatga agaggttgat
     4621 gaaatgatca gggaagcaga cagcgatggg gatggtcagg taaactacga agagtttgta
     4681 caaatgatga cagcgaagta ggtaaattca ctcctcaggt gcaggctgcc tatcagaagg
     4741 tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg
     4801 ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga
     4861 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat
     4921 atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat
     4981 atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga
     5041 aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt
     5101 tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg
     5161 ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc
     5221 tcttatgaag atcggatcgc tactagagga tgcacatgtg accgagggag gagctgcaag
     5281 aactcttcct cacgataact tcgtatagga gactttatac gaagttaagc gctcactggc
     5341 cgtcgtttta cagggacacg aagtgatccg tttaaactat cagtgtttga caggatatat
     5401 tggcgggtaa acctaagaga aaaragcgtt tattagaata atcggatatt taaaagggcg
     5461 tgaaaaggtt tatccgttcg tccatttgta tgtgccagcc gcctttgcga cgctcaccgg
     5521 gctggttgcc ctcgccgctg ggctggcggc cgtctatggc cctgcaaacg cgccagaaac
     5581 gccgtcgaag ccgtgtgcga gacaccgcgg ccgccggcgt tgtggatacc tcgcggaaaa
     5641 cttggccctc actgacagat gaggggcgga cgttgacact tgaggggccg actcacccgg
     5701 cgcggcgttg acagatgagg ggcaggctcg atttcggccg gcgacgtgga gctggccagc
     5761 ctcgcaaatc ggcgaaaacg cctgatttta cgcgagtttc ccacagatga tgtggacaag
     5821 cctggggata agtgccctgc ggtattgaca cttgaggggc gcgactactg acagatgagg
     5881 ggcgcgatcc ttgacacttg aggggcagag tgctgacaga tgaggggcgc acctattgac
     5941 atttgagggg ctgtccacag gcagaaaatc cagcatttgc aagggtttcc gcccgttttt
     6001 cggccaccgc taacctgtct tttaacctgc ttttaaacca atatttataa accttgtttt
     6061 taaccagggc tgcgccctgt gcgcgtgacc gcgcacgccg aaggggggtg cccccccttc
     6121 tcgaaccctc ccggcccgct aacgcgggcc tcccatcccc ccaggggctg cgcccctcgg
     6181 ccgcgaacgg cctcacccca aaaatggcag cgctggccaa ttcccgagtg cgcggaaccc
     6241 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     6301 gataaatgct tcaataatat tgaaaaagga agagtatggc taaaatgaga atatcaccgg
     6361 aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg
     6421 ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc
     6481 ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag
     6541 gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc
     6601 tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg
     6661 aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg
     6721 attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata
     6781 acgatctggc cgatgtggat tgcgaaaact gggaagagga cactccattt aaagatccgc
     6841 gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg
     6901 gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc
     6961 ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgctca
     7021 gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc
     7081 ctgattggga gaaaataaaa tattatattt tactggatga attgttttag ctgtcagacc
     7141 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     7201 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     7261 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     7321 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     7381 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     7441 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     7501 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     7561 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     7621 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     7681 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     7741 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     7801 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     7861 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     7921 tggcagatcc tagatgtggc gcaacgatgc cggcgacaag caggagcgca ccgacttctt
     7981 ccgcatcaag tgttttggct ctcaggccga ggcccacggc aagtatttgg gcaaggggtc
     8041 gctggtattc gtgcagggca agattcggaa taccaagtac gagaaggacg gccagacggt
     8101 ctacgggacc gacttcattg ccgataaggt ggattatctg gacaccaagg caccaggcgg
     8161 gtcaaatcag gaataagggc acattgcccc ggcgtgagtc ggggcaatcc cgcaaggagg
     8221 gtgaatgaat cggacgtttg accggaaggc atacaggcaa gaactgatcg acgcggggtt
     8281 ttccgccgag gatgccgaaa ccatcgcaag ccgcaccgtc atgcgtgcgc cccgcgaaac
     8341 cttccagtcc gtcggctcga tggtccagca agctacggcc aagatcgagc gcgacagcgt
     8401 gcaactggct ccccctgccc tgcccgcgcc atcggccgcc gtggagcgtt cgcgtcgtct
     8461 tgaacaggag gcggcaggtt tggcgaagtc gatgaccatc gacacgcgag gaactatgac
     8521 gaccaagaag cgaaaaaccg ccggcgagga cctggcaaaa caggtcagcg aggccaagca
     8581 ggccgcgttg ctgaaacaca cgaagcagca gatcaaggaa atgcagcttt ccttgttcga
     8641 tattgcgccg tggccggaca cgatgcgagc gatgccaaac gacacggccc gctctgccct
     8701 gttcaccacg cgcaacaaga aaatcccgcg cgaggcgctg caaaacaagg tcattttcca
     8761 cgtcaacaag gacgtgaaga tcacctacac cggcgtcgag ctgcgggccg acgatgacga
     8821 actggtgtgg cagcaggtgt tggagtacgc gaagcgcacc cctatcggcg agccgatcac
     8881 cttcacgttc tacgagcttt gccaggacct gggctggtcg atcaatggcc ggtattacac
     8941 gaaggccgag gaatgcctgt cgcgcctaca ggcgacggcg atgggcttca cgtccgaccg
     9001 cgttgggcac ctggaatcgg tgtcgctgct gcaccgcttc cgcgtcctgg accgtggcaa
     9061 gaaaacgtcc cgttgccagg tcctgatcga cgaggaaatc gtcgtgctgt ttgctggcga
     9121 ccactacacg aaattcatat gggagaagta ccgcaagctg tcgccgacgg cccgacggat
     9181 gttcgactat ttcagctcgc accgggagcc gtacccgctc aagctggaaa ccttccgcct
     9241 catgtgcgga tcggattcca cccgcgtgaa gaagtggcgc gagcaggtcg gcgaagcctg
     9301 cgaagagttg cgaggcagcg gcctggtgga acacgcctgg gtcaatgatg acctggtgca
     9361 ttgcaaacgc tagggccttg tggggtcagt tccggctggg ggttcagcag cccctgctcg
     9421 gatctgttgg accggacagt agtcatggtt gatgggctgc ctgtatcg
//
