LOCUS       sequence-363160-        8370 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CFP fusion of Sec31A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8370)
  AUTHORS   Stephens DJ, Lin-Marq N, Pagano A, Pepperkok R, Paccaud JP
  TITLE     COPI-coated ER-to-Golgi transport complexes segregate from COPII in 
            close proximity to ER exit sites.
  JOURNAL   J Cell Sci. 2000 Jun;113 ( Pt 12):2177-85.
  PUBMED    10825291
REFERENCE   2  (bases 1 to 8370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2000-06"; volume: "113 ( Pt 12)"; pages: "2177-85"
COMMENT     Sequence Label: pECFP-Sec31A
FEATURES             Location/Qualifiers
     source          1..8370
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="cyan variant of GFP"
                     /label=CFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(658..679)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(919..938)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1266..1287
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5158..5279
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5195..5214)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5249..5268
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5286..5741)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5423..5444)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5635..5654
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5768..5872
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5874..6231
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5874..6065
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5897..5917)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6082..6217
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6266..7060
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6266..7060
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6320..6339)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6930..6949
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7248..7267)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7292..7339
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7668..8256
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8157..8176
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aggttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tggggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgtaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac atcagccaca acgtctatat caccgccgac
     1081 aagcagaaga acggcatcaa ggcccacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgagct caagcttcga attcgatgaa gttaaaggaa
     1381 gtagatcgta cagccatgca ggcatggagc cctgcccaga atcaccccat ttacctagca
     1441 acaggaacat ctgctcagca attggatgca acatttagta cgaatgcttc ccttgagata
     1501 tttgaattag acctctctga tccatccttg gatatgaaat cttgtgccac attctcctct
     1561 tctcacaggt accacaagtt gatttggggg ccttataaaa tggattccaa aggagatgtc
     1621 tctggagttc tgattgcagg tggtgaaaat ggaaatatta ttctctatga tccttctaaa
     1681 attatagctg gagacaagga agttgtgatt gcccagaatg acaagcatac tggcccagtg
     1741 agagccttgg atgtgaacat tttccagact aatctggtag cttctggtgc taatgaatct
     1801 gaaatctaca tatgggatct aaataatttt gcaaccccaa tgacaccagg agccaaaaca
     1861 cagccgccag aagatatcag ctgcattgca tggaacagac aagttcagca tattttagca
     1921 tcagccagtc ccagtggccg ggccactgta tgggatctta ggaaaaatga gccaatcatc
     1981 aaagtcagtg accatagtaa cagaatgcat tgttctgggt tggcatggca tcctgatgtt
     2041 gctactcaga tggtccttgc ctccgaggat gaccggttac cagtgatcca gatgtgggat
     2101 cttcgatttg cttcctctcc acttcgtgtc ctggaaaacc atgccagggg gattttggca
     2161 attgcttgga gcatggcaga tcctgaattg ttactgagct gtggaaaaga tgctaagatt
     2221 ctctgctcca atccaaacac aggagaggtg ttatatgaac ttcccaccaa cacacagtgg
     2281 tgcttcgata ttcagtggtg tccccgaaat cctgctgtct tatcagctgc ttcgtttgat
     2341 gggcgtatca gtgtttattc tatcatggga ggaagcacag atggtttaag acagaaacaa
     2401 gttgacaagc tttcatcatc ttttgggaat cttgatccct ttggcacagg acagcccctt
     2461 cctccgttac aaattccaca gcagactgct cagcatagta tagtgctgcc tctgaagaag
     2521 ccgcccaagt ggattcgaag gcctgttggt gcttcttttt catttggagg caaactggtt
     2581 acgtttgaga atgtcagaat gccttctcat cagggagctg agcagcagca gcagcagcac
     2641 catgtgttca ttagtcaggt tgtaacagaa aaggagttcc tcagccgatc agaccaactt
     2701 cagcaggctg tgcagtcaca aggatttatc aattattgcc aaaaaaaaat tgatgcttct
     2761 cagactgaat ttgagaaaaa tgtgtggtcc tttttgaagg taaactttga ggatgattct
     2821 cgtggaaaat accttgaact tctaggatac agaaaagaag atctaggaaa gaagattgct
     2881 ttggccttga acaaagtgga tggagccaat gtggctctta aagactctga ccaagtagca
     2941 cagagtgatg gggaggagag ccctgctgct gaagagcagc tcttgggaga gcacattaaa
     3001 gaggaaaaag aagaatctga atttctaccc tcatctggag gaacatttaa tatctctgtc
     3061 agtggggaca ttgatggttt aattactcag gctttgctga cgggcaattt tgagagtgct
     3121 gttgaccttt gtttacatga taaccgcatg gccgatgcca ttatattggc catagcaggt
     3181 ggacaagaac tcttggctcg aacccagaaa aaatacttcg caaaatccca aagcaaaatt
     3241 accaggctca tcactgcagt ggtgatgaag aactggaaag agattgttga gtcttgtgat
     3301 cttaaaaatt ggagagaggc tttagctgca gtattgactt atgcaaagcc ggatgaattt
     3361 tcagcccttt gtgatctttt gggaaccagg cttgaaaatg aaggagatag cctcctgcag
     3421 actcaagcat gtctctgcta tatttgtgcg gggaatgtag agaaattagt tgcatgttgg
     3481 actaaagctc aagatggaag ccaccctttg tcacttcagg atctgattga gaaagttgtc
     3541 atcctgcgaa aagctgtgca actcactcaa gccatggaca ctagtactgt aggagttctc
     3601 ttggctgcga agatgagtca gtatgccaat ttgttggcag ctcagggcag tattgctgca
     3661 gccttggctt ttcttcctga caacaccaac cagccaaata tcatgcagct tcgtgacaga
     3721 ctttgtagag cacaaggaga gcctgtagca ggacatgaat cacctaaaat tccgtacgag
     3781 aaacagcagc tccccaaggg caggcctgga ccagttgctg gccaccacca gatgccaaga
     3841 gttcaaactc aacaatatta tccccatgga gaaaatcctc cacctccggg tttcataatg
     3901 catggaaatg ttaatccaaa tgctgctggt cagcttccca catctccagg tcatatgcac
     3961 acccaggtac caccttatcc acagccacag ccttatcaac cagcccagcc gtatcccttc
     4021 ggaacagggg ggtcagcaat gtatcgacct cagcagcctg ttgctcctcc tacttcaaac
     4081 gcttacccta acacccctta catatcttct gcttcttcct atactgggca gtctcagctg
     4141 tacgcagcac agcaccaggc ctcttcacct acctccagcc ctgctacttc tttccctcct
     4201 cccccttcct ctggagcatc cttccagcat ggcggaccag gagctccacc atcatcttca
     4261 gcttatgcac tgcctcctgg aacaacaggt acactgcctg ctgccagtga gctgcctgcg
     4321 tcccaaagaa caggtcctca gaatggttgg aatgaccctc cagctttgaa cagagtaccc
     4381 aaaaagaaga agatgcctga aaacttcatg cctcctgttc ccatcacatc accaatcatg
     4441 aacccgttgg gtgaccccca gtcacaaatg ctgcagcaac agccttcagc tccagtacca
     4501 ctgtcaagcc agtcttcatt cccacagcca catcttccag gtggccagcc cttccatggc
     4561 gtacagcaac ctcttggtca aacaggcatg ccaccatctt tttcaaagcc caatattgaa
     4621 ggtgccccag gggctcctat tggaaatacc ttccagcatg tgcagtcttt gccaacaaaa
     4681 aaaattacca agaaacctat tccagatgag cacctcattc taaagaccac atttgaggat
     4741 cttattcagc gctgcctttc ttcagcaaca gaccctcaaa ccaagaggaa gctagatgat
     4801 gccagcaaac gtttggagtt tctgtatgat aaacttaggg aacagacact ttcaccaaca
     4861 atcaccagtg gtttacacaa cattgcaagg agcattgaaa ctcgaaacta ctcagaagga
     4921 ttgaccatgc atacccacat agttagcacc agcaacttca gtgagacctc tgctttcatg
     4981 ccagttctca aagttgttct cacccaggcc aataagctgg gtgtctaagg atccaccgga
     5041 tctagataac tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     5101 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     5161 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     5221 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa
     5281 ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     5341 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     5401 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     5461 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     5521 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     5581 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     5641 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     5701 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg
     5761 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     5821 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc
     5881 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     5941 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     6001 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     6061 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     6121 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     6181 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca
     6241 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     6301 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     6361 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     6421 cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac
     6481 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     6541 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     6601 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     6661 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     6721 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     6781 caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     6841 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     6901 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     6961 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     7021 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     7081 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     7141 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     7201 ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag
     7261 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac
     7321 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat
     7381 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac
     7441 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc
     7501 atagcctcag gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     7561 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     7621 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     7681 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     7741 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     7801 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     7861 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     7921 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     7981 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     8041 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     8101 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     8161 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     8221 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     8281 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     8341 tctgtggata accgtattac cgccatgcat
//
