LOCUS       sequence-363162-        4687 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pSwap plasmid part with H1_alpha homology arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Teufel M, Klein CA, Mager M, Sobetzko P
  TITLE     A multifunctional system for genome editing and large-scale 
            interspecies gene transfer.
  JOURNAL   Nat Commun. 2022 Jun 14;13(1):3430. doi: 10.1038/s41467-022-30843-1.
  PUBMED    35701417
REFERENCE   2  (bases 1 to 4687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-06-14"; volume: "13(1):3430. doi"; pages: " 
            10.1038/s41467-022-30843-1"
COMMENT     Sequence Label: pSWAP_Entry_H1_alpha
FEATURES             Location/Qualifiers
     source          1..4687
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(465..1053)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(545..564)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    1415..1436
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1451..1456,1457..1474,1475..1481)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1489..1505
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1494..1516
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1513..1529
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1513..1529
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1525..1848
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    1542..1598
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1599..1616)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1599..1615)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1608..1630)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1824..1843)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             2190..2495
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2374..2393
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     terminator      complement(4019..4089)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(4107..4125)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4193..4297
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4298..4687,1..405)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
       61 ctggccgatg tggattgcga aaactgggaa gaggacactc catttaaaga tccgcgcgag
      121 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
      181 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
      241 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gctcagggag
      301 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
      361 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagctgtc agaccaagtt
      421 tactccgggg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
      481 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
      541 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
      601 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
      661 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
      721 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
      781 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
      841 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
      901 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
      961 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     1021 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     1081 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     1141 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     1201 tatgagtaaa cttggtctgg cgaaaaaacc ccgccgaagc ggggtttttt gcggtctgac
     1261 tgcagaagac atgtctcgag ttagctagat atcgatacat tataacagtt ccttctgcaa
     1321 atgtcaactc ctatggtgga gtgagaccca gtggtatggg cagctggcac gacaggtttc
     1381 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     1441 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     1501 aacaatttca cacaggaaac agctatgacc atgattacgc caagcttgca tgcctgcagg
     1561 tcgactctag aggatccccg ggtaccgagc tcgaattcac tggccgtcgt tttacaacgt
     1621 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     1681 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     1741 ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     1801 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtc gcgtggatcc
     1861 ggcttactaa aagccagata acagtatgcg tatttgcgcg ctgatttttg cggtataaga
     1921 atatatactg atatgtatac ccgaagtatg tcaaaaagag gtatgctatg aagcagcgta
     1981 ttacagtgac agttgacagc gacagctatc agttgctcaa ggcatatatg atgtcaatat
     2041 ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg ccgaacgctg
     2101 gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg tttattgaaa tgaacggctc
     2161 ttttgctgac gagaacaggg gctggtgaaa tgcagtttaa ggtttacacc tataaaagag
     2221 agagccgtta tcgtctgttt gtggatgtac agagtgatat tattgacacg cccgggcgac
     2281 ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt
     2341 acccggtggt gcatatcggg gatgaaagct ggcgcatgat gaccaccgat atggccagtg
     2401 tgcctgtctc cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca
     2461 aaaacgccat taacctgatg ttctggggaa tataattaag ccagccccga cacccgccaa
     2521 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     2581 tgacccactc aagtgggtct caccgtcagc cgtcaccgtt tggaccttgg tttctacgtg
     2641 ataaaaaaaa ccccgccctg tcaggggcgg ggtttttttt tcgtgttaat ttacccttcc
     2701 aagtttgtac caaacgtttt gtttcttctg gatttgctgc aattcatcgt aaggaaccga
     2761 tgtgaagtac agtttctccg ccatgtctgg cggataaaca cgggggaatt tcttgtgcat
     2821 gtagcgcgtg tagcgcgtct gcaggaagaa aatcaggttc ggattgctca gcgcctcata
     2881 gttggcgatc gccatgtcct gcatggcgtc cgcctgcacc ttgcgctgct gcgtgaattc
     2941 ggacagggca gggcccaggg cattgccgtg cttttccagc agggacacga atacgtggac
     3001 gtcctccagc gccatgttca tgccttgccc gaggaacggg gcggtggcat gcgccgcatc
     3061 gccgatcagc aggatattgg ccttgtagtg gaaggtgctg gaacggacat tgatgaggtc
     3121 gttgctgggc agcgccatga actggtccag catttccttg cgacggtccg gcggcagggt
     3181 gccgaagtag cggctgaaga aatcggccat ggcttcgcgg ttgagcgtgc ccaggctggg
     3241 cgtgccggtg tagggcaggc acagggcaaa gctgatgctg ccgtccggga tggtggccgc
     3301 gcggccggca aacaggccct tggaatccat gccgaagaag tacagcaaat ccttcctgaa
     3361 acccagatcc gccgcgttcg gcaacaccag cgtcttgtag ccgtggcgga agtaactttg
     3421 cctgaactcg aaacggcgca tgccgctttg catggcgcgc cgcacggcag agtgggcgcc
     3481 gtcggcgcca atgaccaggt cgccatgcag cttctgcaag gcgccgtccg ggccctggat
     3541 cagcacgatc tttctttcca ggtcgacatc caggcattta tgctcaaagt aatacttcac
     3601 ctcatgcagg gcggcatgcc ggttcagcag gcgctggaag gcggtgcggt ccagcgacag
     3661 ggggaacagg ccttcgagcg gggtcagctc gcgtatccgg tgccggccgc ccacgctgaa
     3721 tgccatgccg acgatgggtt cgccgcactg gtcgagctcc tgcttgctga tgccggcggc
     3781 caggacggcc ttgatgccgc gtaccgtcat gctcacgccg atggcgcgcg agttgacggg
     3841 gtcggcgttt gcggcattct ccagcagcgg atcgccgcgc ttttcgacaa cgtggacatc
     3901 gtgcccccgt tgcgccagat aaatagcgct gaggctgcct gccaggcctc cgccgacgat
     3961 aatgatttta tgcatttaca aaatcctcag taaaagcccg tctgtcttcc gagcacgtag
     4021 agcgttcacc gacaaacaac agataaaacg aaaggcccag tctttcgact gagcctttcg
     4081 ttttatttga tgcctgggac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     4141 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     4201 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     4261 ctgataaatg cttcaataat attgaaaaag gaagagtatg gctaaaatga gaatatcacc
     4321 ggaattgaaa aaactgatcg aaaaataccg ctgcgtaaaa gatacggaag gaatgtctcc
     4381 tgctaaggta tataagctgg tgggagaaaa tgaaaaccta tatttaaaaa tgacggacag
     4441 ccggtataaa gggaccacct atgatgtgga acgggaaaag gacatgatgc tatggctgga
     4501 aggaaagctg cctgttccaa aggtcctgca ctttgaacgg catgatggct ggagcaatct
     4561 gctaatgagt gaggccgatg gcgtcctttg ctcggaagag tatgaagatg aacaaagccc
     4621 tgaaaagatt atcgagctgt atgcggagtg catcaggctc tttcactcca tcgacatatc
     4681 ggattgt
//
