LOCUS       sequence-363163-        3148 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pSwap plasmid part with insert fragment module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Teufel M, Klein CA, Mager M, Sobetzko P
  TITLE     A multifunctional system for genome editing and large-scale 
            interspecies gene transfer.
  JOURNAL   Nat Commun. 2022 Jun 14;13(1):3430. doi: 10.1038/s41467-022-30843-1.
  PUBMED    35701417
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-06-14"; volume: "13(1):3430. doi"; pages: " 
            10.1038/s41467-022-30843-1"
COMMENT     Sequence Label: pSWAP_Entry_INS
FEATURES             Location/Qualifiers
     source          1..3148
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    118..139
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(154..159,160..177,178..184)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    192..208
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     197..219
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     216..232
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     216..232
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             228..551
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    245..301
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(302..319)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(302..318)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(311..333)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(527..546)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             893..1198
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1077..1096
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     complement(1354..1372)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1440..1544
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1545..2339
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      complement(2399..2987)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2479..2498)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtctggacga agacaaggag acgtgcacag tcgtacctat ggagtgagac ccagtggtat
       61 gggcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
      121 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
      181 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
      241 cgccaagctt gcatgcctgc aggtcgactc tagaggatcc ccgggtaccg agctcgaatt
      301 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
      361 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
      421 gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc
      481 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg
      541 atgccgcata gtcgcgtgga tccggcttac taaaagccag ataacagtat gcgtatttgc
      601 gcgctgattt ttgcggtata agaatatata ctgatatgta tacccgaagt atgtcaaaaa
      661 gaggtatgct atgaagcagc gtattacagt gacagttgac agcgacagct atcagttgct
      721 caaggcatat atgatgtcaa tatctccggt ctggtaagca caaccatgca gaatgaagcc
      781 cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc
      841 cggtttattg aaatgaacgg ctcttttgct gacgagaaca ggggctggtg aaatgcagtt
      901 taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga
      961 tattattgac acgcccgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc
     1021 agataaagtc tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat
     1081 gatgaccacc gatatggcca gtgtgcctgt ctccgttatc ggggaagaag tggctgatct
     1141 cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataatt
     1201 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     1261 ggcatccgct tacagacaag ctgtgaccca ctcaagtggg tctcacgctc ttggtacaag
     1321 tgtggatcac gctctgtctt ccaggacgaa agggcctcgt gatacgccta tttttatagg
     1381 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     1441 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     1501 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatggct aaaatgagaa
     1561 tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa
     1621 tgtctcctgc taaggtatat aagctggtgg gagaaaatga aaacctatat ttaaaaatga
     1681 cggacagccg gtataaaggg accacctatg atgtggaacg ggaaaaggac atgatgctat
     1741 ggctggaagg aaagctgcct gttccaaagg tcctgcactt tgaacggcat gatggctgga
     1801 gcaatctgct aatgagtgag gccgatggcg tcctttgctc ggaagagtat gaagatgaac
     1861 aaagccctga aaagattatc gagctgtatg cggagtgcat caggctcttt cactccatcg
     1921 acatatcgga ttgtccctat acgaatagct tagacagccg cttagccgaa ttggattact
     1981 tactgaataa cgatctggcc gatgtggatt gcgaaaactg ggaagaggac actccattta
     2041 aagatccgcg cgagctgtat gattttttaa agacggaaaa gcccgaagag gaacttgtct
     2101 tttcccacgg cgacctggga gacagcaaca tctttgtgaa agatggcaaa gtaagtggct
     2161 ttattgatct tgggagaagc ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc
     2221 ggtcgctcag ggaggatatc ggggaagaac agtatgtcga gctatttttt gacttactgg
     2281 ggatcaagcc tgattgggag aaaataaaat attatatttt actggatgaa ttgttttagc
     2341 tgtcagacca agtttactcc ggggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     2401 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     2461 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     2521 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     2581 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     2641 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     2701 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     2761 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     2821 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     2881 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     2941 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     3001 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     3061 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     3121 caatctaaag tatatatgag taaacttg
//
