LOCUS       sequence-36319-e         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Ebersbach G, Galli E, Moller-Jensen J, Loewe J, Gerdes K
  TITLE     Novel coiled-coil cell division factor ZapB stimulates Z ring 
            assembly and cell division.
  JOURNAL   Mol Microbiol. 2008 May . 68(3):720-35.
  PUBMED    18394147
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Microbiol. 2008 May . 68(3):720-35."
COMMENT     Sequence Label: pEG3a
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(340..342,343..345,346..858)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(385..406)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(646..665)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gactctgagc ttttttcgca ctctctactg tttctccata cccgtttttt tgggctagcg
       61 aattcgagct cggtacccta aatgacaatg tcattagaag tgtttgagaa actggaagca
      121 aaagtacagc aggcgattga taccatcact ctgttgcaga tggaaatcga agagctgaaa
      181 gaaaaaaaca actcactgtc gcaggaagtt caaaatgccc agcatcagcg cgaagagctg
      241 gagcgtgaga acaaccatct gaaagaacag cagaacggct ggcaggaacg tctgcaggcc
      301 ctgctgggtc gcatggaaga ggtcagcatc cccggcgcca tggtgagcaa gggcgaggag
      361 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
      421 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
      481 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
      541 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
      601 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
      661 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
      721 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
      781 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
      841 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
      901 cccatcggcg acggccccgt gctgctgccc ganaccatct
//
