LOCUS       sequence-363203-        5055 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5055)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 5055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC9A3_STOP
FEATURES             Location/Qualifiers
     source          1..5055
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2612..2711)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2728..2747)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2729..2747)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2752..2768)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2752..2768)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2870..2889)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2881..3690
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2948..2967)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3837..4425
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4326..4345
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4579..4596
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4755..4782)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4874..4960)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5009..5031
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5023..5040
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5024..5040
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgtg gggcctgggc
      121 gccagaggcc cagacagggg cctgctgctg gccctggccc tgggaggact ggcaagggca
      181 ggaggcgtgg aggtggagcc cggcggcgcc cacggcgagt ctggcggctt tcaggtggtg
      241 accttcgagt gggcccacgt gcaggatcct tatgtgatcg ccctgtggat cctggtggca
      301 agcctggcca agatcggctt ccacctgtct cacaaggtga ccagcgtcgt gccagagtcc
      361 gccctgctga tcgtgctggg actggtgctg ggaggaatcg tgtgggccgc cgaccacatc
      421 gccagcttta ccctgacacc caccgtgttc tttttctacc tgctgccccc tatcgtgctg
      481 gatgccggct atttcatgcc taaccggctg tttttcggca atctgggcac aatcctgctg
      541 tatgcagtgg tgggaaccgt gtggaacgca gcaaccacag gactgagcct gtacggcgtg
      601 tttctgtccg gactgatggg cgacctgcag atcggactgc tggattttct gctgttcgga
      661 agcctgatgg cagcagtgga ccctgtggcc gtgctggccg tgttcgagga ggtgcacgtg
      721 aacgaggtgc tgtttatcat cgtgttcggc gagtccctgc tgaacgatgc cgtgacagtg
      781 gtgctgtaca acgtgttcga gagttttgtc gcacttggtg gggacaatgt gaccggcgtg
      841 gattgcgtga agggcatcgt gagcttcttc gtggtgagcc tgggaggcac actggtgggc
      901 gtggtgtttg ccttcctgct gtccctggtg acacgcttta ccaagcacgt gcggatcatc
      961 gagcccggct tcgtgttcat catctcctac ctgtcttatc tgacatctga gatgctgtct
     1021 ctgagcgcca tcctggccat caccttctgt ggcatctgct gtcagaagta cgtgaaggcc
     1081 aacatcagcg agcagtccgc caccacagtg agatatacaa tgaagatgct ggccagctcc
     1141 gccgagacca tcatctttat gttcctgggc atcagcgccg tgaacccctt catctggaca
     1201 tggaataccg cctttgtgct gctgacactg gtgttcatct ccgtgtacag ggcaatcgga
     1261 gtggtgctgc agacctggct gctgaacaga tataggatgg tgcagctgga gcccatcgac
     1321 caggtggtgc tgtcctacgg aggactgagg ggagcagtgg ccttcgccct ggtggtgctg
     1381 ctggacggcg ataaggtgaa ggagaagaat ctgtttgtga gcaccacaat catcgtggtg
     1441 tttttcacag tgatcttcca gggcctgacc atcaagccac tggtgcagtg gctgaaggtg
     1501 aagagatccg agcacaggga gccacggctg aacgagaagc tgcacggcag ggcctttgac
     1561 cacatcctgt ccgccatcga ggatatctct ggccagatcg gccacaatta cctgagggac
     1621 aagtggtccc actttgatcg caagttcctg tctcgggtgc tgatgcggag aagcgcccag
     1681 aagtccaggg accggatcct gaacgtgttc cacgagctga atctgaagga tgccatcagc
     1741 tatgtggcag agggagagag gaggggatcc ctggccttta tcagatcccc ctctaccgac
     1801 aacgtggtga atgtggattt cacacctcgc tctagcaccg tggaggccag cgtgtcctac
     1861 ctgctgcggg agaacgtgag cgccgtgtgc ctggacatgc agtctctgga gcagcggaga
     1921 aggagcatca gggacgccga ggatatggtg acacaccaca ccctgcagca gtacctgtat
     1981 aagcctagac aggagtacaa gcacctgtat tccaggcacg agctgacacc aaccgaggac
     2041 gagaagcagg atagagagat ctttcacagg acaatgagaa agaggctgga gtctttcaag
     2101 agcaccaagc tgggcctgaa ccagaataag aaggccgcca agctgtataa gagggagagg
     2161 gcacagaaga ggaggaactc ctctatccct aatggcaagc tgcctatgga gtctccagcc
     2221 cagaacttca caatcaagga gaaggacctg gagctgagcg ataccgagga gccacccaat
     2281 tacgacgagg agatgagcgg cggcatcgag tttctggcct ccgtgacaaa ggacaccgcc
     2341 tctgatagcc cagccggcat cgataatccc gtgttctctc ctgacgaggc cctggatcgc
     2401 agcctgctgg caaggctgcc tccatggctg tccccaggag agaccgtggt gccatctcag
     2461 agagccagga cacagatccc atacagcccc ggcacctttt gtagactgat gcccttcagg
     2521 ctgagctcca agtccgtgga ctctttcctg caggccgatg gacctgagga gaggccacct
     2581 gccgccctgc cagagtccac ccacatgtaa gacccagctt tcttgtacaa agttggcatt
     2641 ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa
     2701 atcattattt gccatccagc tgatatcccc tatagtgagt cgtattacat ggtcatagct
     2761 gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa
     2821 aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt
     2881 atgagccata ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat
     2941 ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc
     3001 ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc
     3061 aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg
     3121 accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc
     3181 ggaaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat
     3241 gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac
     3301 agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat
     3361 gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg
     3421 cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat
     3481 aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc
     3541 gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca
     3601 ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag
     3661 tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg
     3721 cagagcatta cgctgacttg acgggacggc gcaagctcat gaccaaaatc ccttaacgtg
     3781 agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     3841 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     3901 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     3961 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     4021 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     4081 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     4141 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     4201 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     4261 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     4321 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     4381 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     4441 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     4501 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     4561 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     4621 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     4681 gactggaaag cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag
     4741 tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg
     4801 cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat
     4861 ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac
     4921 gaaaggccca gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact
     4981 ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac gttgtaaaac gacggccagt
     5041 cttaagctcg ggccc
//
