LOCUS       sequence-363204-       12449 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  L-type voltage-gated calcium channel alpha-1C subunit tagged with 
            Dronpa. Fluctuating green-fluorescent label for live-cell 
            super-resolution imaging..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12449)
  AUTHORS   Lin W, Mo GCH, Mehta S, Zhang J
  TITLE     DrFLINC Contextualizes Super-resolution Activity Imaging.
  JOURNAL   J Am Chem Soc. 2021 Sep 22;143(37):14951-14955. doi: 
            10.1021/jacs.1c05530. Epub 2021 Sep 13.
  PUBMED    34516108
REFERENCE   2  (bases 1 to 12449)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.1c05530"; journalName: "J Am Chem Soc"; date: 
            "2021-09-22- 22"; volume: "143"; issue: "37"; pages: "14951-14955"
COMMENT     Sequence Label: pGW-Dronpa-Cav1.2
FEATURES             Location/Qualifiers
     source          1..12449
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        45..424
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        425..628
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     579..599
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     625..649
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          764..1590
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             1635..2306
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /translation="MSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKVK
                     EGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNAT
                     NDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDVNMALSL
                     EGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPRQ
                     AK"
     CDS             complement(3231..3245)
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     polyA_signal    9081..9215
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9118..9137)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9172..9191
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9238..9254)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9238..9254)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9251..9273)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9262..9278
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9286..9292,9293..9310,9311..9316))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(9468..9485)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9639..10227)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9719..9738)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10398..11189,11190..11258))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11021..11040
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     11468..11486
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11524..11546)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11646..11665
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11897..11919
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11911..11928
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11912..11928
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tatgtacatt tatattggct catgtccaac attaccgcca tgttgacatt gattattgac
       61 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
      121 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      181 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      241 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      301 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      361 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      421 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      481 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      541 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      601 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      661 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccgcggccg
      721 ggaacggtgc attggaacgc ggattccccg tgccaagagt gacgtaagta ccgcctatag
      781 agtctatagg cccaccccct tggcttctta tgcatgctat actgtttttg gcttggggtc
      841 tatacacccc cgcttcctca tgttataggt gatggtatag cttagcctat aggtgtgggt
      901 tattgaccat tattgaccac tcccctattg gtgacgatac tttccattac taatccataa
      961 catggctctt tgccacaact ctctttattg gctatatgcc aatacactgt ccttcagaga
     1021 ctgacacgga ctctgtattt ttacaggatg gggtctcatt tattatttac aaattcacat
     1081 atacaacacc accgtcccca gtgcccgcag tttttattaa acataacgtg ggatctccac
     1141 gcgaatctcg ggtacgtgtt ccggacatgg gctcttctcc ggtagcggcg gagcttctac
     1201 atccgagccc tgctcccatg cctccagcga ctcatggtcg ctcggcagct ccttgctcct
     1261 aacagtggag gccagactta ggcacagcac gatgcccacc accaccagtg tgccgcacaa
     1321 ggccgtggcg gtagggtatg tgtctgaaaa tgagctcggg gagcgggctt gcaccgctga
     1381 cgcatttgga agacttaagg cagcggcaga agaagatgca ggcagctgag ttgttgtgtt
     1441 ctgataagag tcagaggtaa ctcccgttgc ggtgctgtta acggtggagg gcagtgtagt
     1501 ctgagcagta ctcgttgctg ccgcgcgcgc caccagacat aatagctgac agactaacag
     1561 actgttcctt tccatgggtc ttttctgcag tcaccgtcct tgacacgaag cttcccggga
     1621 taggtaccgc caccatgagt gtgattaaac cagacatgaa gatcaagctg cgtatggaag
     1681 gcgctgtaaa tggacacccg ttcgcgattg aaggagttgg ccttgggaag cctttcgagg
     1741 gaaaacagag tatggacctt aaagtcaaag aaggcggacc tctgcctttc gcctatgaca
     1801 tcttgacaac tgtgttctgt tacggcaaca gggtattcgc caaataccca gaaaatatag
     1861 tagactattt caagcagtcg tttcctgagg gctactcttg ggaacgaagc atgaattacg
     1921 aagacggggg catttgtaac gcgacaaacg acataaccct ggatggtgac tgttatatct
     1981 atgaaattcg atttgatggt gtgaactttc ctgccaatgg tccagttatg cagaagagga
     2041 ctgtgaaatg ggagccatcc actgagaaat tgtatgtgcg tgatggagtg ctgaagggtg
     2101 atgttaacat ggctctgtcg cttgaaggag gtggccatta ccgatgtgac ttcaaaacta
     2161 cttataaagc taagaaggtt gtccagttgc cagactatca ctttgtggac caccacattg
     2221 agattaaaag ccacgacaaa gattacagta atgttaatct gcatgagcat gccgaagcgc
     2281 attctgagct gccgaggcag gccaaggcta gcatgcttcg agcccttgtt cagccagcta
     2341 cgcccgcata ccagccgctg cctagccacc tgtctgctga aacggagagt acatgtaaag
     2401 gtactgtggt gcatgaagct caactcaacc atttctacat ctctcctgga ggttccaact
     2461 atgggagccc acgcccagct catgccaaca tgaatgccaa cgcagctgcg gggctcgccc
     2521 ctgagcacat ccccacccca ggggcagccc tgtcctggca ggcagccatc gatgcggccc
     2581 ggcaggccaa gctgatgggc agtgctggca acgcgactat ctccaccgtc agctccacgc
     2641 agcggaagcg gcagcagtat gggaagccca agaagcaggg cagcaccact gccactcgcc
     2701 cgccccgtgc cctgctctgc ctcaccctga agaaccccat ccggagggcg tgcataagca
     2761 tcgtcgagtg gaaaccattt gaaataatta ttttactgac tatttttgcc aattgtgtgg
     2821 ccttagcaat ctatattccc tttccagaag atgactccaa tgccaccaat tccaacctgg
     2881 aacgagtgga atatctcttt ctcataattt ttactgtgga agcattttta aaagtaatag
     2941 cctatggact tctgtttcac cccaacgctt acctccgcaa tggctggaat ttactagact
     3001 ttataattgt ggttgtaggg ctttttagtg caattttaga acaagcaacc aaagcagacg
     3061 gggccaatgc cctaggaggg aaaggggctg gattcgacgt gaaggcgctg agggctttcc
     3121 gcgtgctgcg ccccctgcgg ctggtgtctg gagtcccgag tctccaggtg gtcctgaact
     3181 ccatcatcaa ggccatggtc cctctgctgc acattgccct gctagtgctg tttgtcatca
     3241 tcatctatgc catcatcggc ctggagctct tcatggggaa gatgcacaag acatgctaca
     3301 accaggaggg tgtagcagat gtcccagcag aagatgatcc ttccccttgt gctctggaga
     3361 cgggccacgg gcggcagtgc cagaacggca ccgtgtgcaa gcctgggtgg gatggaccca
     3421 agcacggcat caccaacttt gacaattttg ctttcgccat gttgacggtg ttccagtgta
     3481 tcaccatgga gggctggacc gacgtgctgt actggatgca ggacgctatg ggctatgagc
     3541 taccctgggt gtattttgtc agtctggtca tctttggatc ctttttcgtt ctaaatctgg
     3601 ttctcggtgt gttgagcgga gagttttcca aagagaggga gaaggccaaa gctcggggag
     3661 atttccagaa gttgcgggag aagcagcagc tggaagagga cctcaaaggc tacctggact
     3721 ggatcactca ggcagaagac atcgaccctg agaatgagga tgaaggcatg gatgaggaga
     3781 aaccccgaaa catgagcatg cccacaagtg agaccgaatc tgtcaacact gaaaacgtgg
     3841 ctggaggtga catcgaagga gaaaactgcg gggccaggct ggcccaccgg atctccaagt
     3901 cgaaattcag ccgctactgg cgccggtgga ataggttctg caggagaaag tgccgcgcag
     3961 cggtcaagtc gaacgtcttc tactggctgg tgatcttcct ggtcttcctg aacacgctca
     4021 ccattgcctc tgagcactac aaccagcccc actggctcac ggaggtccaa gacacggcca
     4081 ataaggctct actggccctg ttcactgccg agatgctgct gaagatgtac agcctgggcc
     4141 tgcaggccta tttcgtgtcc ctcttcaacc gcttcgactg cttcattgtg tgcgggggca
     4201 tcctggagac catcctggtg gagaccaagg tcatgtcccc cctgggcatc tctgtgctga
     4261 gatgcgtgcg gctcctgaga atattcaaaa ttacaaggta ctggaactcc ttgagcaacc
     4321 tggtggcctc cctgctgaac tcggtgcgct ccatcgcctc cctgctcctg ctcctcttcc
     4381 tcttcatcat catcttctcc ctgctgggga tgcagctgtt tggaggcaag ttcaacttcg
     4441 atgagatgca gacccggagg agcacgttcg acaatttccc gcagtccctg ctcaccgtgt
     4501 ttcagatcct gaccggggag gactggaatt cggtgatgta tgatgggatc atggcttatg
     4561 gcggcccctc ttttccaggg atgttagtct gtatttactt catcatcctc ttcatctgtg
     4621 gaaattatat cctactgaat gtgttcttgg ccattgctgt ggacaacctg gctgatgctg
     4681 agagccttac ttctgcccaa aaggaagagg aagaagagaa ggagagaaag aagctggcca
     4741 ggactgccag cccggagaag aaacaagagg tggtagggaa gccggccctg gaggaggcca
     4801 aggaggagaa aattgagctg aaatccatta cagctgatgg agagtccccg cctaccacca
     4861 agatcaacat ggatgacctc cagcccaatg agagtgagga taagagtccc taccccaacc
     4921 cggaaaccac aggagaagag gatgaggagg agcctgagat gcctgtcggc ccccgccctc
     4981 ggccactctc cgagctgcac cttaaggaga aggccgtgcc tatgccagaa gccagtgcgt
     5041 ttttcatctt cagccccaac aacaggttcc gcctccagtg tcaccgtatc gtcaacgaca
     5101 cgatcttcac caacctgatc ctcttcttca ttctgctcag cagcatttcc ctggctgccg
     5161 aggaccctgt gcagcacacc tccttcagga atcacattct gttttatttt gacattgttt
     5221 ttactaccat tttcaccatt gaaattgctc tcaagatgac tgcgtatggg gccttcctgc
     5281 acaagggctc tttctgcagg aactacttca acatcctgga cctgctggtg gtcagcgtgt
     5341 ccctcatctc cttcggcatc cagtccagcg cgatcaatgt cgtgaagatc ttgcgagtgc
     5401 tgcgagtgct caggccactg cgggccatca acagggccaa ggggctaaag cacgtggttc
     5461 agtgtgtgtt cgtggccatc cggaccattg ggaacatcgt gattgtctac acgctgctga
     5521 tgttcatgtt cgcctgcatc ggagtccagc tcttcaaggg gaagctgtac acctgttcag
     5581 acagttccaa acagactgag gctgaatgca agggtaacta catcacctac aaagatggag
     5641 aggttgacca tcccatcatc cagccgcgca gctgggagaa cagcaagttt gactttgaca
     5701 acgtcctggc agccatgatg gccctcttca ctgtctccac cttcgagggc tggccagagc
     5761 tgctgtaccg ctccatcgac tcccacacgg aagacaaggg ccctatctac aactaccgag
     5821 tggagatctc catcttcttc atcatctaca tcatcatcat cgccttcttc atgatgaaca
     5881 tcttcgtggg tttcgtcatt gtcaccttcc aggagcaggg ggagcaggag tacaagaact
     5941 gtgagctgga caagaaccag cggcagtgcg tggaatatgc cctcaaggcc cggcccctgc
     6001 ggaggtacat ccccaagaac cagcaccagt acaaagtgtg gtacgtggtc aactccacct
     6061 actttgagta cctgatgttc gtcctcatcc tgctcaacac catctgcttg gccatgcagc
     6121 actacggcca gagctgcctg ttcaaaatcg ccatgaacat cctcaacatg ctcttcaccg
     6181 gcctcttcac cgtggaaatg atcctgaagc tcattgcctt caaacccaag ggttacttta
     6241 gtgatccctg gaatgttttt gacttcctca tcgtaattgg cagcataatt gacgtcattc
     6301 tcagtgagac taatccagct gaacataccc aatgctctcc ctctatgaac gcagaggaga
     6361 actcccgcat ctccatcacc ttcttccgcc tgttccgggt catgcgcctg gtcaagctgc
     6421 tgagccgcgg ggagggcatc cggacgctgc tgtggacctt catcaagtcc ttccaggccc
     6481 tgccctatgt ggctcttctg atcgtaatgc tgttcttcat ctatgctgtg atcgggatgc
     6541 aggtgtttgg gaaaatcgcc ctgaacgaca ccacggagat caaccggaac aacaacttcc
     6601 agaccttccc ccaagctgtg ctgctcctct tcaggtgtgc cacgggggag gcttggcagg
     6661 atatcatgct ggcctgcatg ccaggcaaga agtgtgcccc agagtctgag ccccacaaca
     6721 gcacagaagg ggagaccccc tgcggcagca gcttcgccgt cttctacttc atcagcttct
     6781 acatgctttg tgccttcctg atcatcaatc tctttgtagc tgtcatcatg gacaactttg
     6841 actacctgac aagggactgg tcaatccttg gtccccacca tctggatgaa tttaaaagaa
     6901 tctgggcaga gtatgaccct gaagccaagg gtcgtatcaa acacctggat gtggtgaccc
     6961 tcctccggcg gattcagccc ccactgggtt ttgggaagct gtgccctcac cgtgtggctt
     7021 gcaaacgcct ggtctccatg aacatgcctc tgaacagtga cgggacggtc atgttcaacg
     7081 ccaccctgtt tgccctggtc aggacagctc tgaggatcaa aacagaagga aacctggaac
     7141 aagccaatga ggagctgcgg gccatcatca agaagatctg gaagcggacc agcatgaagc
     7201 tgctggacca agtggtgccc cctgcaggcg atgatgaggt cacagtcggc aagttctacg
     7261 ctaccttcct gatccaagag tacttccgga aattcaagaa gcgcaaagag caagggcttg
     7321 tgggcaagcc ctcccagagg aacgcccttt ccctgcaggc tggcctgcgc actctgcacg
     7381 acatcgggcc tgagatccga cgggccatct ccggagacct gacagctgag gaagagctgg
     7441 acaaggccat gaaggaggct gtgtctgctg cctctgaaga tgacatcttc aggagggccg
     7501 gtggcctgtt tggcaaccat gtcagctact accaaagtga cagccggagc gcctttcccc
     7561 agaccttcac tacgcagcgc ccactgcaca tcagcaaggc tggcaacaac caaggcgaca
     7621 ccgagtcacc ctcccacgag aagctggtgg actccacttt cacccccagc agctactcgt
     7681 ccaccggctc caacgccaac atcaacaatg ccaacaacac tgccctgggc cgcctccccc
     7741 gccccgccgg ctaccccagc acagtcagca ccgtggaggg ccacgggtcc cccttgtctc
     7801 ctgccgtccg ggcacaggag gcagcatgga agctcagctc caagagatgc cactcccagg
     7861 agagccagat agccatggcg tgtcaggagg gcgcatccca ggacgacaac tacgacgtga
     7921 ggatcggtga agatgcagag tgctgcagtg agcccagcct gctctccaca gagatgctct
     7981 cctaccagga tgacgaaaac cgacaactgg cgcccccgga ggaggagaag cgggacatca
     8041 ggctgtctcc aaagaagggt ttcctgcgct ccgcgtcact gggtcgaagg gcttccttcc
     8101 acctggagtg tctgaagcgg cagaagaatc aagggggaga catctctcag aagacagtcc
     8161 tgcccctgca tctggtccac caccaggcat tggcagtggc gggcctgagt cccctcctgc
     8221 agagaagcca ttcccccacc tcgctcccta ggccctgtgc cacgccccct gccacaccgg
     8281 gcagccgagg ctggccccca cagcccatcc ccaccctgcg gctggagggg gccgactcca
     8341 gtgagaaact caacagcagc ttcccgtcca tccactgcgg ctcatggtct ggggagaaca
     8401 gcccctgcag aggggacagc agcgccgccc ggagagcccg gcccgtctcc ctcactgtgc
     8461 ccagccaggc tggggcccag gggagacagt tccacggcag cgccagcagc ctggtggaag
     8521 cggtcttgat ttccgaagga ctggggcagt ttgctcaaga tcccaagttc atcgaggtca
     8581 cgacccagga gctggctgac gcctgcgatc tgaccataga ggagatggag aacgcggccg
     8641 acgacattct cagcgggggc gcccggcaga gccccaatgg caccctgtta ccctttgtga
     8701 accgcaggga cccgggccgg gacagagcgg ggcagaacga gcaggacgcg agcggcgcat
     8761 gcgccccagg gtgcgggcag agcgaggagg ccctcgcgga ccgcagggcc ggcgtcagca
     8821 gcctgtaggc gccagggccg ggggtgcggg ttttttattt gtctcaatgt tcctaatggg
     8881 ttcgtttcag aagtgcctca ctgttctcgt gacctggagt taaccgcgga attggggatc
     8941 ctctagagtc gacctgcagg atatcgaatt cattgatcat aatcagccat accacatttg
     9001 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     9061 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     9121 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     9181 ccaaactcat caatgtatct tatcatgtct ggatccgtac cgagctcgcg taatcatgtc
     9241 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     9301 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactacatt aattgcgttg
     9361 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     9421 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     9481 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     9541 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     9601 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     9661 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     9721 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     9781 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     9841 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     9901 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     9961 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
    10021 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
    10081 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
    10141 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
    10201 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
    10261 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
    10321 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
    10381 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
    10441 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
    10501 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
    10561 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
    10621 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
    10681 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
    10741 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
    10801 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    10861 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    10921 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    10981 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    11041 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    11101 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    11161 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    11221 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaataagcg
    11281 gccgcggcca tgccggccac tagtctcgag ttattattga agcatttatc agggttattg
    11341 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    11401 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
    11461 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
    11521 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
    11581 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
    11641 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgaagacgt cgcctcctca
    11701 ctacttctgg aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat
    11761 tagtcagggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
    11821 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
    11881 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc ccgtgtcaag
    11941 gacggtgact gcagtgaata ataaaatgtg tgtttgtccg aaatacgcgt tttgagattt
    12001 ctgtcgccga ctaaattcat gtcgcgcgat agtggtgttt atcgccgata gagatggcga
    12061 tattggaaaa atcgatattt gaaaatatgg catattgaaa atgtcgccga tgtgagtttc
    12121 tgtgtaactg atatcgccat ttttccaaaa gtgatttttg ggcatacgcg atatctggcg
    12181 atagcgctta tatcgtttac gggggatggc gatagacgac tttggtgact tgggcgattc
    12241 tgtgtgtcgc aaatatcgca gtttcgatat aggtgacaga cgatatgagg ctatatcgcc
    12301 gatagaggcg acatcaagct ggcacatggc caatgcatat cgatctatac attgaatcaa
    12361 tattggccat tagccatatt attcattggt tatatagcat aaatcaatat tggctattgg
    12421 ccattgcata cgttgtatcc atatcataa
//
