LOCUS       sequence-363232-        8713 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral transfer plasmid for expression of NucGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8713)
  AUTHORS   Rogers LC, Zhou J, Baker A, Schutt CR, Panda PK, Van Tine BA
  TITLE     Intracellular arginine-dependent translation sensor reveals the 
            dynamics of arginine starvation response and resistance in 
            ASS1-negative cells.
  JOURNAL   Cancer Metab. 2021 Jan 21;9(1):4. doi: 10.1186/s40170-021-00238-9.
  PUBMED    33478587
REFERENCE   2  (bases 1 to 8713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Metab."; date: "2021-01-21"; volume: "9(1):4. doi"; pages: " 
            10.1186/s40170-021-00238-9"
COMMENT     Sequence Label: pKLV2-EF1a-NucGFP
FEATURES             Location/Qualifiers
     source          1..8713
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    522..639
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(937..981)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1166..1399
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1898..2023
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             2070..2250
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(2268..2466)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2293..2313)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(2781..2800)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2782..2800)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2810..2827)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2810..2826)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2819..2841)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2968..3423
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3055..3074)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3265..3286
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3449..3553
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3554..3622,3623..4414)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3772..3791)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4585..5173
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5074..5093
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5327..5344
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5461..5482
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5497..5502,5503..5520,5521..5527)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5535..5551
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5540..5562
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5559..5575
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5559..5575
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5594..5614
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5596..5614
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    5640..5864
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     5853..5870
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             6322..6333
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6731..6751
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6814..6834)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(6835..7545,7546..7548,7549..7551))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(6835..7545,7546..7548,7549..7551))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6877..6898)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     7226..7245
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7485..7506
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(join(7583..8713,1..51))
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7591..8533
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(7614..7634)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 ctcggggact gtgggcgatg tgcgctctgc ccactgacgg gcaccggagc ctcacgcatg
       61 ctcttctcca cctcagtgat gacgagagcg ggcgggtgag ggggcgggaa cgcagcgatc
      121 tctgggttct acgttagtgg gagtttaacg acggtccctg ggattcccca aggcaggggc
      181 gagtcctttt gtatgaatta ctctcagctc cggtcggggc gggttggggg gggtggtgac
      241 ggggaggccg cctggaaggg acgtgcagaa tcttccctct accattgctg gcttagctcc
      301 aaaggttgta ttgagattag ggtgtacctt cgcctctcaa tcagcctccc gtcctcagcc
      361 ttgccatctc gctagtccgg gacaaatccc tagagcgtct tcctctgcgg gtctcagccc
      421 agcccggggt tggctcctcc tccgccccgg cttccgcgcc cctcacgcgt agggcccggt
      481 actaaccaaa ctggatctct gctgtccctg taataaaccc gaaaattttg aatttttgta
      541 atttgttttt gtaattcttt agtttgtatg tctgttgcta ttatgtctac tattctttcc
      601 cctgcactgt accccccaat cccccctttt cttttaaaat tgtggatgaa tactgccatt
      661 tgtctgcaga attggcgcac gcagtgccga tccgttcact aatcgaatgg atctgtctct
      721 gtctctctct ccaccttctt cttctattcc ttcgggcctg tcgggtcccc tcggggttgg
      781 gaggtgggtc tgaaacgata atggtgaata tccctgccta actctattca ctatagaaag
      841 tacagcaaaa actattctta aacctaccaa gcctcctact atcattatga ataattttat
      901 ataccacagc caatttgtta tgttaaacca attccacaaa cttgcccatt tatctaattc
      961 caataattct tgttcattct tttcttgctg gttttgcgat tcttcaatta aggagtgtat
     1021 taagcttgtg taattgttaa tttctctgtc ccactccatc caggtcgtgt gattccaaat
     1081 ctgttccaga gatttattac tccaactagc attccaaggc acagcagtgg tgcaaatgag
     1141 ttttccagag caaccccaaa tccccaggag ctgttgatcc tttaggtatc tttccacagc
     1201 caggattctt gcctggagct gcttgatgcc ccagactgtg agttgcaaca gatgctgttg
     1261 cgcctcaata gccctcagca aattgttctg ctgctgcact ataccagaca ataattgtct
     1321 ggcctgtacc gtcagcgtca ttgacgctgc gcccatagtg cttcctgctg ctcccaagaa
     1381 cccaaggaac aaagctccta ttcccactgc tcttttttct ctctgcacca ctcttctctt
     1441 tgccttggtg ggtgctactc ctaatggttc aatttttact actttatatt tatataattc
     1501 acttctccaa ttgtccctca tatctcctcc tccaggtctg aagatcagcg cggccggccg
     1561 cttgctgtgc ggtggtctta cttttgtttt gctcttcctc tatcttgtct aaagcttcct
     1621 tggtgtcttt tatctctatc ctttgatgca cacaatagag ggttgctact gtattatata
     1681 atgatctaag ttcttctgat cctgtctgaa gggatggttg tagctgtccc agtatttgtc
     1741 tacagccttc tgatgtttct aacaggccag gattaactgc gaatcgttct agctccctgc
     1801 ttgcccatac tatatgtttt aatttatatt ttttctttcc ccctggcctt aaccgaattt
     1861 tttcccatcg cgatctaatt ctcccccgct taatactgac gctctcgcac ccatctctct
     1921 ccttctagcc tccgctagtc aaaatttttg gcgtactcac cagtcgccgc ccctcgcctc
     1981 ttgccgtgcg cgcttcagca agccgagtcc tgcgtcgaga gagctcctct ggtttccctt
     2041 tcgctttcaa gtccctgttc gggcgccact gctagagatt ttccacactg actaaaaggg
     2101 tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta cttgaagcac
     2161 tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     2221 ggctcagatc tggtctaacc agagagaccc agtacaggca aaacgcgctg cttatataga
     2281 cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt
     2341 ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca atggggtgga
     2401 gacttggaaa tccccgtgag tcaaaccgct atccacgccc attgatgtac tgccaaaacc
     2461 gcatcaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc
     2521 ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag
     2581 ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat
     2641 actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga acatacgtca
     2701 ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa
     2761 gttatgtact agtcaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
     2821 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     2881 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     2941 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     3001 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     3061 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     3121 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     3181 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     3241 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     3301 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     3361 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     3421 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     3481 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     3541 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     3601 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     3661 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     3721 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     3781 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     3841 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     3901 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     3961 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     4021 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     4081 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     4141 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     4201 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     4261 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     4321 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     4381 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     4441 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     4501 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     4561 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     4621 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     4681 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     4741 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     4801 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     4861 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     4921 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     4981 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     5041 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     5101 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     5161 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     5221 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     5281 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     5341 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     5401 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     5461 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc tcccggctcg
     5521 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     5581 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagcttaatt aacggccgcc
     5641 catagagccc accgcatccc cagcatgcct gctattgact tcccaatcct cccccttgct
     5701 gtcctgcccc accccacccc ccagaataga atgacaccta ctcagacaat gcgatgcaat
     5761 ttcctcattt tattaggaaa ggacagtggg agtggcacct tccagggtca aggaaggcac
     5821 gggggagggg caaacaacag atggctggca actagaaggc acagtcgagg ctgatcagcg
     5881 ggtttaaact gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac
     5941 cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag
     6001 gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc
     6061 agagagactc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgacttct
     6121 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     6181 agctgccttg taagtcattg gtcttaaagg tacctgcggg gaggcggccc aaagggagat
     6241 ccgactcgtc tgagggcgaa ggcgaagacg cggaagaggc cgcagagccg gcagcaggcc
     6301 gcgggaagga aggtccgctg gattgagggc cgaagggacg tagcagaagg acgtcccgcg
     6361 cagaatccag gtggcaacac aggcgagcag ccaaggaaag gacgatgatt tccccgacaa
     6421 caccacggaa ttgtcagtgc ccaacagccg agcccctgtc cagcagcggg caaggcaggc
     6481 ggcgatgagt tccgccgtgg caatagggag ggggaaagcg aaagtcccgg aaaggagctg
     6541 acaggtggtg gcaatgcccc aaccagtggg ggttgcgtca gcaaacacag tgcacaccac
     6601 gccacgttgc ctgacaacgg gccacaactc ctcataaaga gacagcaacc aggatttata
     6661 caaggaggag aaaatgaaag ccatacggga agcaatagca tgatacaaag gcattaaagc
     6721 agcgtatcca catagcgtaa aaggagcaac atagttaaga ataccagtca atctttcaca
     6781 aattttgtaa tccagaggtt gagcggccgc ttatacctta cgcttcttct ttggcttgta
     6841 cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg
     6901 atcgcgcttc tcgttggggt ctttgctcag ggcggactgg gtgctcaggt agtggttgtc
     6961 gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg
     7021 cacgctgccg tcctcgatgt tgtggcggat cttgaagttc accttgatgc cgttcttctg
     7081 cttgtcggcc atgatataga cgttgtggct gttgtagttg tactccagct tgtgccccag
     7141 gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc
     7201 gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt
     7261 gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg
     7321 gtcggggtag cggctgaagc actgcacgcc gtaggtcagg gtggtcacga gggtgggcca
     7381 gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc
     7441 atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc
     7501 gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tggatcccgc
     7561 catggtgggc gcgccaagct tccacgacac ctgaaatgga agaaaaaaac tttgaaccac
     7621 tgtctgaggc ttgagaatga accaagatcc aaactcaaaa agggcaaatt ccaaggagaa
     7681 ttacatcaag tgccaagctg gcctaacttc agtctccacc cactcagtgt ggggaaactc
     7741 catcgcataa aacccctccc cccaacctaa agacgacgta ctccaaaagc tcgagaacta
     7801 atcgaggtgc ctggacggcg cccggtactc cgtggagtca catgaagcga cggctgagga
     7861 cggaaaggcc cttttccttt gtgtgggtga ctcacccgcc cgctctcccg agcgccgcgt
     7921 cctccatttt gagctccctg cagcagggcc gggaagcggc catctttccg ctcacgcaac
     7981 tggtgccgac cgggccagcc ttgccgccca gggcggggcg atacacggcg gcgcgaggcc
     8041 aggcaccaga gcaggccggc cagcttgaga ctacccccgt ccgattctcg gtggccgcgc
     8101 tcgcaggccc cgcctcgccg aacatgtgcg ctgggacgca cgggccccgt cgccgcccgc
     8161 ggccccaaaa accgaaatac cagtgtgcag atcttggccc gcatttacaa gactatcttg
     8221 ccagaaaaaa agcgtcgcag caggtcatca aaaattttaa atggctagag acttatcgaa
     8281 agcagcgaga caggcgcgaa ggtgccacca gattcgcacg cggcggcccc agcgcccagg
     8341 ccaggcctca actcaagcac gaggcgaagg ggctccttaa gcgcaaggcc tcgaactctc
     8401 ccacccactt ccaacccgaa gctcgggatc aagaatcacg tactgcagcc aggggcgtgg
     8461 aagtaattca aggcacgcaa gggccataac ccgtaaagag gccaggcccg cgggaaccac
     8521 acacggcact tacctgtgtt ctggcggcaa acccgttgcg aaaaagaacg ttcacggcga
     8581 ctactgcact tatatacggt tctcccccac cctcgggaaa aaggcggagc cagtacacga
     8641 catcactttc ccagtttacc ccgcgccact ttctctaggc accggttcaa ttgccgaccc
     8701 ctccccccaa ctt
//
