LOCUS       sequence-363290-       13678 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multiplex luciferase reporter vector, with luciferase reporters for 
            the pathways HRE, P53, AP-1, FOXO and SMAD..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13678)
  AUTHORS   Sarrion-Perdigones A, Gonzalez Y, Venken KJT
  TITLE     Synthetic Assembly DNA Cloning of Multiplex Hextuple Luciferase 
            Reporter Plasmids.
  JOURNAL   Methods Mol Biol. 2022;2524:409-432. doi: 
            10.1007/978-1-0716-2453-1_32.
  PUBMED    35821490
REFERENCE   2  (bases 1 to 13678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2022"; volume: "2524:409-432. doi"; pages: " 
            10.1007/978-1-0716-2453-1_32"
COMMENT     Sequence Label: MLRV3:HRE-P53-MAPK/JNK-FOXO-TGF-beta
FEATURES             Location/Qualifiers
     source          1..13678
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    14..238
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        complement(1955..2158)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1984..2004)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2159..2462
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    2540..2588
                     /note="synthetic polyadenylation signal"
     misc_feature    2602..2693
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     promoter        2848..2879
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     polyA_signal    4564..4788
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    4801..4849
                     /note="synthetic polyadenylation signal"
     misc_feature    4863..4954
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     promoter        5067..5098
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             5132..6784
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(5198..5216)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    6792..7016
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    7029..7077
                     /note="synthetic polyadenylation signal"
     misc_feature    7091..7182
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     promoter        7305..7336
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     polyA_signal    8310..8534
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    8547..8595
                     /note="synthetic polyadenylation signal"
     misc_feature    8609..8700
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     promoter        8848..8879
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     polyA_signal    9433..9657
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    9670..9718
                     /note="synthetic polyadenylation signal"
     misc_feature    9732..9823
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     promoter        9984..10015
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     polyA_signal    10989..11213
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(11321..11909)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11401..11420)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             12377..13168
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      13223..13651
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13310..13329)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13520..13541
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 taacggatca gcgccataga gcccaccgca tccccagcat gcctgctatt gtcttcccaa
       61 tcctccccct tgctgtcctg ccccacccca csccccagaa tagaatgaca cctactcaga
      121 caatgcgatg caatttcctc attttattag gaaaggacag tgggagtggc accttccagg
      181 gtcaaggaag gcacggggga ggggcaaaca acagatggct ggcaactaga aggcacagaa
      241 gctcaagccg ccagggcata gttctcatcg ttggcggcgg atgtcttgta catagatcca
      301 ccacccgcct tctccataag tgcttctcgg agttcctttc ggctgatttt gcccgtgacg
      361 ttcctcggaa tagagtcaac gaatctgaca ccccctcgaa gatacttgct gtggctaact
      421 ctttgtgcaa ggaaatcata tacttcttta gccgtcagtt gggccccagg ttgcttgaca
      481 acgaatccgg ccggaagttc ccctgcttct atgtcgggga taccgactac ggccacatcg
      541 cggatccctg gatgttggag caaaatctcc tccaactcga caggagccac ttgatatccc
      601 ttgtacttga tcagctcttt atatctatcc acaatgtaaa aatattcatc ctcatcatag
      661 tagccaaaat caccactgtg aagccaacca tcgtcgtcaa tggcctcctt ggtggcctgt
      721 ggattattca cgtagccttt cgtgaccatg gggccccaaa tgcacaactc tcccacttga
      781 tttgggccaa gcgcctctcc ggtgtttcga tctataattt tagcggccat gtagggcgtc
      841 acttttccca atgaaccgga tttaaattcg ttgtgaagag tgtggatatt agcggatgtg
      901 ctttcagtca acccgtaacc acaccgtatg cctggcaaat taaggcgttt tacggcgatt
      961 tcagctactt cttttgcaag aggtgccgca ccacaacaaa gttccgccaa ggtagaaaga
     1021 tcatacttat caaccaacgg tgatttagaa agaaagagga ttgtacttgg gacattgatc
     1081 acacttcgta cttcatagtc ttgaatcgcc ttcaaaaaaa cctcttggtt gaacctccga
     1141 agcatcacga cgcgcagccc caccataaaa taccccaagt taatggaaaa cccaaaggcg
     1201 tgaaagaagg gaagatacgc cagcacacta actccgggaa tgagttgggt accaactctc
     1261 gggtcagagg cgtgggtcag ccgaacacat atattgcggt gggtctgcat gacgccctta
     1321 ggcaacccgg tagtaccact actacacagt atcgctgcaa cctggtcgac cggatcgtaa
     1381 tgcagtggtt taaaggtctg aagattgtta tcgctatatc ttgacataaa attatgcaag
     1441 gattcacatc cgagaaggtt ttcctcagag tccagaatta tgattctttt gatataattt
     1501 gttctatcct taacctccaa aacctttgga agtatctttc gcgtggtgaa cacaagtatt
     1561 ggcttactaa ttcctgtgac cttacacagt tcgtctggta tatagtcctc gtttacaggt
     1621 gcaacgacca tgcctatata ccacgcacta ataattggaa tgaaaaatct tttgttgttt
     1681 tcagcgcata ttgatacaac atcattcatt ttatagccac aattctggag actctgcccc
     1741 aatttgaccg tcgtgtcgaa gaattcctga taactaagac tttcgttacc catcacgtcg
     1801 aggatcgcct gcgggatgtg actatgtttg tgaagcgcgt tgtagagcat ctctccagcg
     1861 gtaaaattcc caagcgggtg cttaggctcg ggcccataca caacattctt ctctctcttc
     1921 atcatttgag cttgatctga cggttcacta aaccagctct gcttatatag acctcccacc
     1981 gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat tttggaaagt
     2041 cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg agacttggaa
     2101 atccccgtga gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac cgcatcacca
     2161 tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc cataaggtca
     2221 tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata gggggcgtac
     2281 ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa tactccaccc
     2341 attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc attattgacg
     2401 tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta agttatgtaa
     2461 cgcggaactc catatatggg ctatgaacta atgaccccgt aattgattac tattaataac
     2521 tactccggat cccagggaga ataaaatatc tttattttca ttacatctgt gtgttggttt
     2581 tttgtgtgaa tcgatagtac taacatacgc tctccatcaa aacaaaacga aacaaaacaa
     2641 actagcaaaa taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc tctcgctgtc
     2701 aggaggtgac tacgtgctgc ctagagatct gtgactacgt gctgcctagg cggccgcgtg
     2761 actacgtgct gcctagtcta gagtgactac gtgctgccta gtccctcgag gatatcaaat
     2821 ctggcctcgg cggccaagct tagacactag agggtatata atggaagctc gacttccagc
     2881 ttggcaatcc ggtactgttg gtaaagccac caatggagaa catggagaac gacgagaaca
     2941 ttgtggtggg acccaagccg ttctacccga tcgaggaggg ttccgctggc acccagctgc
     3001 gcaagtacat ggagcgctac gctaagctgg gtgctatcgc cttcaccaac gccgtgaccg
     3061 gtgtggatta ctcctacgcc gagtacctgg agaagtcctg ctgcctcgga aaggccctgc
     3121 agaactacgg tctggtcgtg gatggccgca ttgccctctg ctcggagaac tgcgaggagt
     3181 tcttcattcc cgtgatcgct ggtctgttca tcggagtcgg cgttgcccca accaacgaga
     3241 tctacaccct gcgcgagctg gtccacagcc tgggtatcag caagccgacc atcgttttct
     3301 cgagcaagaa gggcctcgac aaggttatca ccgtgcaaaa aaccgtcacc accatcaaga
     3361 ccatcgttat cctggattcc aaggttgact accgcggtta ccagtgcctc gacaccttca
     3421 tcaagcgtaa caccccaccc ggcttccaag cctcctcctt caaaaccgtg gaggttgatc
     3481 gcaaggagca ggtggccctg atcatgaact cctccggctc caccggtctg ccaaagggcg
     3541 tgcagctgac ccacgagaac accgtcaccc gcttctcgca tgcccgtgat cccatctacg
     3601 gcaaccaggt gtccccaggc accgccgtcc tgaccgtcgt gcccctccac cacggcttcg
     3661 gcatgttcac cactctcggc tacttgatct gcggcttccg tgtggtgatg ctgactaagt
     3721 tcgacgagga gactttcctc aagaccctgc aagactacaa gtgcacttac gtgatcctgg
     3781 tgccgaccct gttcgccatc ctgaacaaga gcgagctgtt gaacaaatac gacctgtcga
     3841 acctggtgga gatcgcttcc ggcggcgccc cactgtcgaa ggaagttggc gaggccgttg
     3901 ctcgccgctt caacctgccc ggcgtccgtc agggttacgg cctgaccgag actacctcgg
     3961 ctatcatcat cacccccgag ggcgacgata agcccggcgc ttccggcaag gttgttccac
     4021 tcttcaaagc caaggtgatc gacctggaca ccaagaagtc cctgggtccc aaccgtcgcg
     4081 gagaagtgtg cgtcaagggc cccatgctga tgaagggcta cgtcaacaac ccagaggcca
     4141 ccaaggagtt gatcgacgag gagggctggc tgcacactgg tgacatcggt tactacgacg
     4201 aagagaagca cttcttcatc gttgaccgcc tgaagtcctt gatcaagtac aagggctacc
     4261 aggttccccc cgccgagctc gagagcgtcc tgctgcagca cccctccatt ttcgatgccg
     4321 gagtggccgg tgtccccgat cccgtggctg gcgagctgcc aggtgccgtc gtcgtgctgg
     4381 agtccggcaa gaacatgacc gagaaggaag tgatggatta cgtggcctcg caagtgtcca
     4441 acgccaagcg cctgcgcggc ggcgtgcgct tcgtggatga ggtgcccaag ggcctcaccg
     4501 gaaagatcga cggtcgcgct atccgcgaga tcctcaagaa gcccgtggcc aagatgtgag
     4561 cttctgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     4621 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     4681 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     4741 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggcg ctgtcaggag
     4801 aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga atcgatagta
     4861 ctaacatacg ctctccatca aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc
     4921 cccagtgcaa gtgcaggtgc cagaacattt ctctcgctgt caggagtaca gaacatgtct
     4981 aagcatgctg tgccttgcct ggacttgcct ggccttgcct tccctcgagg atatcaaatc
     5041 tggcctcggc ggccaagctt agacactaga gggtatataa tggaagctcg acttccagct
     5101 tggcaatccg gtactgttgg taaagccacc aatggaagac gccaaaaaca taaagaaagg
     5161 cccggcgcca ttctatccgc tggaagatgg aaccgctgga gagcaactgc ataaggctat
     5221 gaagagatac gccctggttc ctggaacaat tgcttttaca gatgcacata tcgaggtgga
     5281 catcacttac gctgagtact tcgaaatgtc cgttcggttg gcagaagcta tgaaacgata
     5341 tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc aattctttat
     5401 gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg acatttataa
     5461 tgaacgtgaa ttgctcaaca gtatgggcat ttcgcagcct accgtggtgt tcgtttccaa
     5521 aaaggggttg caaaaaattt tgaacgtgca aaaaaagctc ccaatcatcc aaaaaattat
     5581 tatcatggat tctaaaacgg attaccaggg atttcagtcg atgtacacgt tcgtcacatc
     5641 tcatctacct cccggtttta atgaatacga ttttgtgcca gagtccttcg atagggacaa
     5701 gacaattgca ctgatcatga actcctctgg atctactggt ctgcctaaag gtgtcgctct
     5761 gcctcataga actgcctgcg tgagattctc gcatgccaga gatcctattt ttggcaatca
     5821 aatcattccg gatactgcga ttttaagtgt tgttccattc catcacggtt ttggaatgtt
     5881 tactacactc ggatatttga tatgtggatt tcgagtcgtc ttaatgtata gatttgaaga
     5941 agagctgttt ctgaggagcc ttcaggatta caagattcaa agtgcgctgc tggtgccaac
     6001 cctattctcc ttcttcgcca aaagcactct gattgacaaa tacgatttat ctaatttaca
     6061 cgaaattgct tctggtggcg ctcccctctc taaggaagtc ggggaagcgg ttgccaagag
     6121 gttccatctg ccaggtatca ggcaaggata tgggctcact gagactacat cagctattct
     6181 gattacaccc gagggggatg ataaaccggg cgcggtcggt aaagttgttc cattttttga
     6241 agcgaaggtt gtggatctgg ataccgggaa aacgctgggc gttaatcaaa gaggcgaact
     6301 gtgtgtgaga ggtcctatga ttatgtccgg ttatgtaaac aatccggaag cgaccaacgc
     6361 cttgattgac aaggatggat ggctacattc tggagacata gcttactggg acgaagacga
     6421 acacttcttc atcgttgacc gcctgaagtc tctgattaag tacaaaggct atcaggtggc
     6481 tcccgctgaa ttggaatcca tcttgctcca acaccccaac atcttcgacg caggtgtcgc
     6541 aggtcttccc gacgatgacg ccggtgaact tcccgccgcc gttgttgttt tggagcacgg
     6601 aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc agtcaagtaa caaccgcgaa
     6661 aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg aaaggtctta ccggaaaact
     6721 cgacgcaaga aaaatcagag agatcctcat aaaggccaag aagggcggaa agatcgccgt
     6781 gtaatgagct tctgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     6841 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     6901 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     6961 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcgct
     7021 aatgggagaa taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat
     7081 cgatagtact aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat
     7141 aggctgtccc cagtgcaagt gcaggtgcca gaacatttct ctcgctgcag actatgagtc
     7201 aaacagcgtg actcaagttg agtcagttga ctcacttgag tcactgactc aggcatgctc
     7261 cctcgaggat atcaaatctg gcctcggcgg ccaagcttag acactagagg gtatataatg
     7321 gaagctcgac ttccagcttg gcaatccggt actgttggta aagccaccaa tgacttccaa
     7381 ggtttatgac cccgagcaac gcaagagaat gataactggg cctcagtggt gggccagatg
     7441 taaacaaatg aatgtccttg atagtttcat caattactac gacagtgaaa agcatgcaga
     7501 aaacgccgta atttttttgc atggcaacgc agcctcctct tatttgtggc gacacgtagt
     7561 cccgcatata gagccggtag ctaggtgtat tatccctgat ctgatcggaa tgggaaagag
     7621 tggcaagtca gggaatggat cttatcgcct tcttgaccac tacaagtatc tgacggcttg
     7681 gttcgaactc ttgaatttgc cgaagaaaat catcttcgtg ggtcacgatt ggggagcctg
     7741 tctcgcgttc cattattcct acgagcatca ggacaagata aaggcaatcg ttcacgccga
     7801 gtctgtagta gatgtaatcg agtcctggga cgaatggcca gatattgagg aggatatagc
     7861 attgattaaa agcgaggaag gggaaaagat ggtgctggag aacaacttct ttgtggaaac
     7921 aatgcttcct tcaaaaatca tgagaaaact ggaacccgag gaatttgccg cttatctcga
     7981 accgtttaaa gaaaagggag aagtccgcag gccaacactt tcctggccaa gagaaatccc
     8041 gctcgtcaaa ggcggaaagc cggatgttgt gcaaatagtc cgaaattaca acgcgtacct
     8101 ccgggcctct gacgatctcc ctaaaatgtt tatagaatca gatccaggat tttttagcaa
     8161 cgctatcgta gaaggagcaa agaaatttcc aaataccgaa tttgtcaaag taaagggact
     8221 gcatttcagt caagaggacg ctccagacga gatgggcaaa tacataaagt cctttgtaga
     8281 gagggtcctg aagaacgaac agtgagcttc tgtgccttct agttgccagc catctgttgt
     8341 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     8401 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     8461 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     8521 ggtgggctct atggcgctgc ttggagaata aaatatcttt attttcatta catctgtgtg
     8581 ttggtttttt gtgtgaatcg atagtactaa catacgctct ccatcaaaac aaaacgaaac
     8641 aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga acatttctct
     8701 cgctgaattc gatcaagtaa acaactatgt aaacaagatg cagatcaagt aaacaactat
     8761 gtaaacaact ccgttgatca agtaaacaac tatgtaaaca atccctcgag gatatcaaat
     8821 ctggcctcgg cggccaagct tagacactag agggtatata atggaagctc gacttccagc
     8881 ttggcaatcc ggtactgttg gtaaagccac caatggtgtt taccttggag gacttcgtgg
     8941 gtgactggag gcaaactgca ggatacaatt tggaccaggt cctcgaacag ggaggagtca
     9001 gcagcctttt ccaaaacttg ggcgtgtccg ttacccctat tcagagaatt gtcctttccg
     9061 gagagaacgg attgaaaata gatatccacg tcataatccc gtatgaagga ctgtctgggg
     9121 accaaatggg tcagattgaa aaaatattca aagtagttta tcccgttgat gatcatcact
     9181 tcaaagttat ccttcattat gggactttgg taatcgatgg agttactcct aatatgatag
     9241 attactttgg ccgaccttac gaggggatcg ctgtattcga cggcaagaaa atcaccgtca
     9301 ctggaaccct ctggaacggc aacaagatta ttgacgagcg attgatcaat ccagacggat
     9361 ctttgctttt ccgcgtaacg ataaacggcg taacagggtg gaggctctgc gaacgaatac
     9421 tcgcctgagc ttctgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     9481 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     9541 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     9601 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggcgc
     9661 ttggcggaga ataaaatatc tttattttca ttacatctgt gtgttggttt tttgtgtgaa
     9721 tcgatagtac taacatacgc tctccatcaa aacaaaacga aacaaaacaa actagcaaaa
     9781 taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc tctcgctgtc aggaggtcta
     9841 gactgccgtc tagacttaac agtacgtcta gactcggccg tctagactta gtacgtctag
     9901 actcggtatg tctagactga cccgtctaga cggatcctcc ctcgaggata tcaaatctgg
     9961 cctcggcggc caagcttaga cactagaggg tatataatgg aagctcgact tccagcttgg
    10021 caatccggta ctgttggtaa agccaccaat ggcttccaaa gtctacgacc cagaacaaag
    10081 gaagaggatg attactggac cgcagtggtg ggcaaggtgc aagcagatga atgtactcga
    10141 tagttttatc aattattacg actccgaaaa acacgcggaa aatgctgtca tattcctgca
    10201 cgggaacgcc actagcagct acttgtggcg gcacgtagtc ccccacatag agcctgttgc
    10261 ccgatgtatt attcccgatc tcattggaat gggcaaatca ggcaaatctg ggaacggttc
    10321 ttaccgcctg ctcgatcact ataaatattt gaccgcttgg tttgagctgc ttaatctccc
    10381 taaaaaaata atattcgttg gacacgactg gggctcagct ctggcgttcc actacgcata
    10441 cgagcatcaa gaccgaataa aagcaatagt acatatggag tcagttgttg acgtcataga
    10501 gtcatggatg ggttggccag atattgaaga ggaattggcc ctcatcaaga gtgaggaggg
    10561 ggaaaaaatg gtcctggaaa ataatttttt cgtggaaacc ttgctcccga gtaaaataat
    10621 gcgaaaactt gaacccgaag agtttgctgc ttatttggag cctttcaagg aaaaggggga
    10681 agtaagacga ccaacccttt cttggccgag ggagatacct cttgttaagg gtggaaagcc
    10741 cgacgtagtc cagatagtcc gaaattacaa cgcgtacctg agagctagtg atgacctccc
    10801 taagttgttt atcgagtcag accctgggtt cttcagcaac gcaattgtag aaggagcgaa
    10861 aaaatttcca aatacagaat ttgtgaaggt taaggggctc cactttttgc aagaggacgc
    10921 ccccgatgag atgggaaaat acattaaatc tttcgtagag cgcgtcctta agaatgaaca
    10981 gtgagcttct gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
    11041 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
    11101 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
    11161 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcgcttga
    11221 cacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
    11281 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
    11341 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
    11401 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
    11461 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
    11521 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
    11581 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
    11641 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
    11701 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
    11761 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
    11821 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
    11881 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
    11941 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
    12001 tcttcaccta gatccttttg tgaccagcca ggacagaaat gcctcgactt cgctgctacc
    12061 caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc agtggacata
    12121 agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag
    12181 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga
    12241 ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt
    12301 gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa
    12361 aacaaagtta aacattatga gggaagcggt gatcgccgaa gtatcgactc aactatcaga
    12421 ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg
    12481 ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac
    12541 cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc
    12601 ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga
    12661 catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa
    12721 tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct
    12781 gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga
    12841 tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc
    12901 gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta
    12961 cagcgcagta accggcaaaa tcgcgccgaa ggatgtagct gccgactggg caatggagcg
    13021 cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga
    13081 agaagatcgt ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg
    13141 cgagatcacc aaggtagtcg gcaaataatc actagaccaa tgttacacat atatacttta
    13201 gattgattta aaacttcatg ttacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
    13261 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
    13321 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
    13381 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
    13441 ggtgatggtt cacgtagtgg gccagcgccc tgatagacgg tttttcgccc tttgacgttg
    13501 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
    13561 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
    13621 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaattt
//
