LOCUS       sequence-36335-e         727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Zhu C, Smith T, McNulty J, Rayla AL, Lakshmanan A, Siekmann AF, 
            Buffardi M, Meng X, Shin J, Padmanabhan A, Cifuentes D, Giraldez AJ,
            Look AT, Epstein JA, Lawson ND, Wolfe SA
  TITLE     Evaluation and application of modularly assembled zinc-finger 
            nucleases in zebrafish.
  JOURNAL   Development. 2011 Oct;138(20):4555-64.
  PUBMED    21937602
REFERENCE   2  (bases 1 to 727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2011-10"; volume: "138(20)"; pages: "4555-64"
COMMENT     Sequence Label: pCS2-F1-CAG-F2-GAC
FEATURES             Location/Qualifiers
     source          1..727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      5..14
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             17..37
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             38..103
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 atctgccacc atggctccaa agaagaagcg taaggtagac tacaaagacc atgacggtga
       61 ttataaagat catgacatcg attacaagga tgacgatgac aagggtaccc gcccatatgc
      121 ttgccctgtc gagtcctgtg accgccgctt cagccgtagc gacaacctct tggagcatat
      181 ccggattcac acaggccaga agcccttcca gtgtcgcatc tgcatgagga acttcagctt
      241 gaaggggaac ctcacccgcc acatccgcac ccacacaggc gagaagcctt ttgcatgcga
      301 catctgcggc aggaagttcg cgcataggca gtcgctcacc cgccacacca agatccacac
      361 aggtggatcc caactagtca aaagtgaact ggaggagaag aaatctgaac ttcgtcataa
      421 attgaaatat gtgcctcatg aatatattga attaattgaa attgccagaa attccactca
      481 ggatagaatt cttgaaatga aggtaatgga attttttatg aaagtttatg gatatagagg
      541 taaacatttg ggtggatcaa ggaaaccgga cggagcaatt tatactgtcg gatctcctat
      601 tgattacggt gtgatcgtgg atactaaagc ttatagcgga ggttataatc tgccaattgg
      661 ccaagcagat gaaatgcaag actatgtcga agaaaatcaa acacgaaaca aacatatcaa
      721 ccctaat
//
