LOCUS       sequence-363363-        4041 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF232 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR223-ZNF232
FEATURES             Location/Qualifiers
     source          1..4041
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1352..1451)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1468..1487)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1469..1487)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1492..1508)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1492..1508)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1939..2730
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2823..3411
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3312..3331
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3565..3582
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3741..3768)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3860..3946)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3995..4017
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4009..4026
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4010..4026
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca tggctgtatc actaacagca
      121 gctgaaactc tggcccttca gggtacacag ggacaagaga agatgatgat gatgggacca
      181 aaggaagagg aacagtcttg tgagtatgag accaggctac ctgggaacca ctctaccagt
      241 caagagatct tccgccaacg cttcaggcat ctccgctacc aggagactcc tggtccccgg
      301 gaggccttga gccaactacg agtactctgc tgtgagtggc tgaggccaga gaaacacacg
      361 aaggagcaga tcctggagtt cctggtgctg gaacaattct tgaccatcct gcctgaggag
      421 ctccaatcct gggtgcgggg acatcaccct aagagtggag aggaggctgt gactgtgctg
      481 gaggatttag agaaaggact tgaaccagag ccgcaggtcc caggccctgc acatggacct
      541 gcacaggaag agccatggga gaagaaggaa tctctgggag cagcccagga agcactgagc
      601 atccagctcc agcctaagga gacccagcct ttcccaaaga gtgaacaggt atatttacat
      661 tttctgtcag ttgttacaga agatggccca gagcccaagg acaaaggatc attgccacaa
      721 ccacccatta ctgaagtgga atcacaggtg ttctcagaaa aacttgctac tgacacctct
      781 acatttgaag ctacctctga gggtacctta gaactgcagc agagaaatcc caaagcggag
      841 agactgaggt ggtcccctgc ccaggaggaa agtttcaggc agatggttgt catccataag
      901 gaaattccca cagggaagaa agaccatgaa tgtagtgaat gtggtaaaac cttcatttat
      961 aactcacatc ttgttgtcca ccagagagtt cattctggag agaaacccta taagtgtagt
     1021 gactgtggga aaactttcaa acagagctca aacctcggtc agcatcagag aattcataca
     1081 ggagagaaac ccttcgaatg taatgaatgt gggaaggcct tcagatgggg tgctcatctt
     1141 gttcagcatc agaggattca ctcaggagag aagccctatg agtgtaatga gtgtgggaag
     1201 gcctttagtc aaagctcata tctaagtcag catcggagaa ttcacagtgg agagaaacct
     1261 tttatatgta aagaatgtgg gaaagcttat ggatggtgct cagagctcat tagacatcgg
     1321 agagttcatg ccagaaaaga gccttcccat tgcccaactt tcttgtacaa agttggcatt
     1381 ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa
     1441 atcattattt gccatccagc tgatatcccc tatagtgagt cgtattacat ggtcatagct
     1501 gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa
     1561 aatatatcat catgcctcct ctagaccagc caggacagaa atgcctcgac ttcgctgcta
     1621 cccaaggttg ccgggtgacg cacaccgtgg aaacggatga aggcacgaac ccagtggaca
     1681 taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg caactggtcc
     1741 agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat
     1801 gactgttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc
     1861 gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct
     1921 aaaacaaagt taaacattat gagggaagcg gtgatcgccg aagtatcgac tcaactatca
     1981 gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac
     2041 ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg
     2101 accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg
     2161 gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac
     2221 gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc
     2281 aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg
     2341 ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt
     2401 gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac
     2461 tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg
     2521 tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag
     2581 cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa
     2641 gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa
     2701 ggcgagatca ccaaggtagt cggcaaataa ccctcgagcc acccatgacc aaaatccctt
     2761 aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     2821 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2881 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2941 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     3001 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     3061 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     3121 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     3181 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     3241 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3301 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3361 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3421 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     3481 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     3541 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     3601 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     3661 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag
     3721 gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat
     3781 gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt
     3841 tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga
     3901 taaaacgaaa ggcccagtct tccgactgag cctttcgttt tatttgatgc ctggcagttc
     3961 cctactctcg cgttaacgct agcatggatg ttttcccagt cacgacgttg taaaacgacg
     4021 gccagtctta agctcgggcc c
//
