LOCUS       sequence-363364-        4179 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF331 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR223-ZNF331
FEATURES             Location/Qualifiers
     source          1..4179
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1490..1589)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1606..1625)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1607..1625)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1630..1646)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1630..1646)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2077..2868
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2961..3549
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3450..3469
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3703..3720
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3879..3906)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3998..4084)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4133..4155
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4147..4164
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4148..4164
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca tggcccaggg tttggtgacg
      121 ttcgccgacg tagccataga cttttctcag gaggagtggg cctgtctgaa ctctgctcag
      181 agggacctgt actgggacgt gatgctggag aactacagta acttggtctc actggatttg
      241 gagtcagcat atgaaaataa gagtttacct acaaaaaaaa acattcatga aataagggct
      301 tccaaaagga attcagatag aagaagtaaa tcccttggcc gtaactggat atgtgaaggt
      361 acgcttgaaa gaccacagcg ctccagaggg aggtatgtca atcagatgat catcaattat
      421 gtcaaaaggc ctgctactag agaaggcacc cctcctagaa cacatcagag acatcataag
      481 gagaattcct ttgaatgtaa ggactgtggg aaggccttta gtcgtggcta tcaacttagt
      541 caacatcaga aaatccatac tggtgagaaa ccttatgaat gtaaagaatg taagaaggcc
      601 ttccgttggg gcaatcagct tactcaacat caaaaaattc atactgggga gaagccctac
      661 gaatgtaaag actgtgggaa ggcttttcga tggggctcaa gcctcgttat tcataagagg
      721 attcatactg gtgaaaaacc ctatgaatgt aaagactgtg gaaaggcctt tcggcgtggt
      781 gatgagctca ctcagcacca gagattccac actggggaga aagactacga atgcaaagac
      841 tgtgggaaga cctttagccg tgtgtataaa cttattcagc acaagagaat tcatagtggg
      901 gagaagcctt acgagtgtaa agactgtggg aaggctttta tttgtggttc aagcctcatt
      961 cagcataaaa gaattcacac aggtgagaaa ccctatgaat gtcaagaatg tgggaaggcc
     1021 tttactcgag tcaattacct tactcagcat cagaagatcc acaccggtga gaagcctcac
     1081 gaatgtaagg agtgtgggaa ggcctttcgc tggggttcga gcctcgttaa gcacgagagg
     1141 atacatacgg gcgagaagcc gtacaagtgc acagaatgtg ggaaggcctt caattgtggc
     1201 tatcacctca ctcagcacga gagaatccac acaggcgaaa ccccgtataa atgtaaggag
     1261 tgtgggaagg ctttcattta tggatcgagc ctcgtgaaac atgagagaat tcataccggg
     1321 gtgaaaccct atgggtgtac agaatgtggg aagagcttta gtcacggcca tcagcttaca
     1381 caacatcaga aaacgcacag tggggcgaaa tcctacgaat gtaaggagtg cgggaaggca
     1441 tgtaaccacc taaaccatct ccgagaacat cagaggatcc acaacagttg cccaactttc
     1501 ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc
     1561 actatcagtc aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg
     1621 tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt
     1681 tacattgcac aagataaaaa tatatcatca tgcctcctct agaccagcca ggacagaaat
     1741 gcctcgactt cgctgctacc caaggttgcc gggtgacgca caccgtggaa acggatgaag
     1801 gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg
     1861 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
     1921 gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag
     1981 cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg
     2041 cagcagggca gtcgccctaa aacaaagtta aacattatga gggaagcggt gatcgccgaa
     2101 gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg
     2161 ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt
     2221 gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac
     2281 gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc
     2341 accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa
     2401 tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac
     2461 attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca
     2521 gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa
     2581 accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt
     2641 acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct
     2701 gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga
     2761 caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa
     2821 tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataacc ctcgagccac
     2881 ccatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta
     2941 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     3001 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     3061 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     3121 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     3181 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     3241 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     3301 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     3361 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     3421 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     3481 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     3541 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     3601 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     3661 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     3721 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     3781 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     3841 acgcgtaccg ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg
     3901 ccttctgctt agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg
     3961 gccgttgctt cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt
     4021 tcaccgacaa acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta
     4081 tttgatgcct ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca
     4141 cgacgttgta aaacgacggc cagtcttaag ctcgggccc
//
