LOCUS       sequence-363365-        4344 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF449 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4344)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR223-ZNF449
FEATURES             Location/Qualifiers
     source          1..4344
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1655..1754)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1771..1790)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1772..1790)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1795..1811)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1795..1811)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2242..3033
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3126..3714
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3615..3634
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3868..3885
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4044..4071)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4163..4249)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4298..4320
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4312..4329
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4313..4329
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca tggctgtggc cctgggttgt
      121 gcaatccagg catccttgaa tcaaggctct gtgtttcaag aatatgatac tgactgtgaa
      181 gttttccgtc agcgcttcag gcagttccag tacagagaag cagctgggcc tcatgaagca
      241 tttaacaaac tctgggagct ttgctgtcaa tggctgaagc caaagatgcg ctctaaggaa
      301 caaatcctgg agctgctagt gttggagcaa ttcctaacta tcctgcccac agagatagag
      361 acctgggtga gggagcactg cccagagaat agagaaagag ttgtgtcact gatagaagac
      421 ttacagagag aacttgagat accagagcag caggttgata tgcatgacat gctcttggaa
      481 gaactggcac cagtgggaac ggcacacata ccaccaacca tgcacctaga gtcacctgca
      541 ctccaggtaa tgggacctgc ccaggaggcc ccagtagcag aggcatggat cccacaggca
      601 gggccaccgg agctgaacta tggtgctact ggagaatgtc agaactttct ggaccctgga
      661 tatccattac caaaacttga catgaacttc tcattggaga atagagaaga gccatgggtg
      721 aaggaattac aggattctaa agaaatgaaa caattacttg attccaagat aggttttgag
      781 atcgggatag aaaatgaaga agatacttca aaacagaaaa aaatggagac tatgtatcca
      841 tttattgtaa ctttagaggg gaatgctctc cagggtccca ttttgcaaaa agactatgta
      901 cagttagaaa atcaatggga aaccccccca gaggatttac agacagattt agcaaaactg
      961 gtagatcagc agaaccccac tctgggagag acacctgaga actccaactt ggaagaacct
     1021 ctcaacccta aaccccacaa gaaaaagagt ccaggagaga aacctcaccg atgtcctcag
     1081 tgtggaaaat gttttgctcg gaagtcacaa cttactgggc atcagagaat tcattcagga
     1141 gaagaacctc acaaatgccc tgaatgtggg aaaagattcc ttcgtagttc agacctttat
     1201 agacaccaac gacttcatac aggggagaga ccctatgaat gcactgtatg taaaaagcga
     1261 ttcactcggc ggtcacatct tatagggcac cagagaaccc attctgaaga agaaacatat
     1321 aaatgtcttg agtgtgggaa aagtttttgt catggatcaa gtcttaaaag acatctgaaa
     1381 actcatacag gtgaaaaacc tcatagatgt cataattgtg ggaaaagttt tagtcgactg
     1441 acagctctta ctttgcacca gagaacgcat actgaagaga gaccttttaa atgtaattat
     1501 tgtgggaaaa gttttagaca gagaccaagc ctcgttattc atttaagaat ccacacaggg
     1561 gagaagccat acaagtgtac tcattgttct aaaagcttca gacagagagc cggccttatt
     1621 atgcaccagg tcactcactt tagaggactt atttacccaa ctttcttgta caaagttggc
     1681 attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat
     1741 aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata
     1801 gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat
     1861 aaaaatatat catcatgcct cctctagacc agccaggaca gaaatgcctc gacttcgctg
     1921 ctacccaagg ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg aacccagtgg
     1981 acataagcct gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg
     2041 tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt
     2101 tatgactgtt tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac
     2161 gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc
     2221 cctaaaacaa agttaaacat tatgagggaa gcggtgatcg ccgaagtatc gactcaacta
     2281 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
     2341 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
     2401 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
     2461 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
     2521 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
     2581 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
     2641 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
     2701 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
     2761 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     2821 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     2881 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga
     2941 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg
     3001 aaaggcgaga tcaccaaggt agtcggcaaa taaccctcga gccacccatg accaaaatcc
     3061 cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     3121 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     3181 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     3241 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     3301 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     3361 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     3421 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     3481 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     3541 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     3601 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     3661 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     3721 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     3781 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     3841 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     3901 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     3961 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc
     4021 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt
     4081 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa
     4141 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
     4201 agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag
     4261 ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg
     4321 acggccagtc ttaagctcgg gccc
//
