LOCUS       sequence-363366-        4005 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF785 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR223-ZNF785
FEATURES             Location/Qualifiers
     source          1..4005
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1316..1415)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1432..1451)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1433..1451)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1456..1472)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1456..1472)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1903..2694
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2787..3375
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3276..3295
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3529..3546
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3705..3732)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3824..3910)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3959..3981
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3973..3990
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3974..3990
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca tggggccgcc cctggcgccg
      121 cgcccggccc acgtacccgg ggaggccggg ccccggagga cgagggaaag caggccgggc
      181 gccgtgagct tcgcggacgt ggccgtgtac ttctctcccg aggagtggga atgcctgcgg
      241 ccagcgcaga gggccctgta ccgggacgtg atgcgggaga ccttcggcca cctgggcgcg
      301 ctgggatttt cagttcccaa acccgctttt atctcgtggg tggaaggaga agtggaggcg
      361 tggagcccgg aggcccagga tcccgacggt gagagctctg cagctttcag caggggccaa
      421 ggacaggaag caggatccag ggatgggaat gaggagaagg aaaggctgaa gaagtgtcca
      481 aaacaaaaag aggtggcgca tgaagtggct gtcaaggagt ggtggcccag cgtcgcctgc
      541 ccagagttct gcaaccctag gcagagcccc atgaatccct ggctcaagga cactctgacc
      601 cgaagactgc cccactcttg cccagactgt ggccgcaact tcagctaccc ttccctcctg
      661 gccagccacc agcgggtcca ctccggggag cggcccttct cctgcggcca gtgtcaggcg
      721 cgtttctccc agcgcaggta cctgctccag catcagttca tccacaccgg cgagaagccc
      781 tacccctgcc ccgactgcgg gcgccgcttc cgccagaggg gttccctggc tatccacagg
      841 cgggctcaca ccggggagaa gccttacgcg tgctcagact gcaagagtcg cttcacttac
      901 ccctacctgc tggccatcca ccagcgcaag cacacgggcg agaagcccta cagctgcccc
      961 gattgcagcc tccgtttcgc ctacacctcc ctgctggcca tccacaggcg catacacacc
     1021 ggcgagaagc cctacccctg tcctgactgc ggccgccgct tcacctattc ttccctcctc
     1081 ctcagtcacc ggcgcattca ctccgacagc cggcccttcc cctgcgtgga gtgtgggaaa
     1141 ggcttcaagc gcaagaccgc cctggaagcc catcggtgga tccaccgctc ctgcagcgag
     1201 aggcgcgcgt ggcagcaggc cgtggtgggg cgttcagagc ccatccctgt tttgggaggc
     1261 aaggatcccc cagttcactt ccggcacttt ccagatatat ttcaagagtg tgggtgccca
     1321 actttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa
     1381 caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt
     1441 gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc
     1501 tgatgttaca ttgcacaaga taaaaatata tcatcatgcc tcctctagac cagccaggac
     1561 agaaatgcct cgacttcgct gctacccaag gttgccgggt gacgcacacc gtggaaacgg
     1621 atgaaggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag
     1681 cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca
     1741 gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca
     1801 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat
     1861 gttacgcagc agggcagtcg ccctaaaaca aagttaaaca ttatgaggga agcggtgatc
     1921 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg
     1981 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt
     2041 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg
     2101 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta
     2161 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa
     2221 ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg
     2281 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta
     2341 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta
     2401 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta
     2461 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat
     2521 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa
     2581 gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg
     2641 gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg
     2701 agccacccat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac
     2761 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     2821 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     2881 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     2941 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     3001 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     3061 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     3121 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     3181 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     3241 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     3301 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     3361 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     3421 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     3481 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     3541 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     3601 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     3661 attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca
     3721 ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc
     3781 ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga
     3841 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc
     3901 gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc
     3961 cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccc
//
