LOCUS       sequence-363369-        4401 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF698 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4401)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR223-ZNF698
FEATURES             Location/Qualifiers
     source          1..4401
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1712..1811)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1828..1847)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1829..1847)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1852..1868)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1852..1868)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2299..3090
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3183..3771
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3672..3691
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3925..3942
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4101..4128)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4220..4306)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4355..4377
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4369..4386
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4370..4386
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca ccatgtttga acagctgaag
      121 ccaatcgaac ctgtacagaa gacgctccca tgggtgggcg aggtagctgc caccctgcag
      181 gaagccatga agagagattg ctggagggag gcacgggtga agaagaagcc agtcaccttt
      241 gaggatgtgg cagtgaattt cacccaggaa gagtgggact gtctagatgc cagccagagg
      301 gtcctttacc aggatgttat gtcggaaacc tttaagaatc taacatctgt ggccagaatc
      361 tttctgcata agccagagct aatcaccaag cttgagcaag aagaggaaca gtggagagag
      421 tttgtccatc tcccaaacac agaaggcctt tcagaaggca agaagaaaga gcttcgagaa
      481 caacatccca gtctgagaga tgaggggact agtgatgaca aggtcttcct tgcatgcaga
      541 ggggccggcc agtgccccct atctgcccca gctgggacta tggacaggac ccgggtgctt
      601 caagcatccc aggctgggcc accctttttt tgctacacct gtggcaaatg tttcagcagg
      661 cgctcctacc tctatagcca ccagtttgtt cacaatccca agctgactaa cagctgcagt
      721 cagtgtggga agttgtttcg gagccccaag tccctcagct atcacagacg catgcatctt
      781 ggggagaggc ccttctgttg cacgttctgt gacaagacct actgtgatgc ttctggacta
      841 agtcgtcacc gccgcgtcca tctgggttac cggccccatt catgctctgt gtgtgggaag
      901 agcttccggg accagtctga gctcaaacgc caccagaaga tacaccaaaa ccaggagcca
      961 gtggatggaa accaggagtg tactttgagg attccaggca cccaggctga attccagaca
     1021 cccatcgcca gaagccagag gtccatccag gggcttttgg atgtgaacca tgcaccagtg
     1081 gccaggtccc aggaacccat atttagaact gagggtccta tggcccagaa ccaggcatct
     1141 gtacttaaga accaagcacc tgtgaccagg acccaggcac ccatcactgg aaccctctgt
     1201 caggatgcca gatccaactc tcatccagtg aagccctcaa gactcaatgt cttctgttgc
     1261 ccccattgtt ctttgacttt tagcaagaaa tcctatctct ccagacacca gaaggcccac
     1321 ctcacagagc cgcccaacta ctgcttccat tgcagcaagt ctttcagctc attttccagg
     1381 ctggtcagac accagcagac ccactggaag cagaagagct acctttgccc tatctgtgac
     1441 ctctcctttg gggagaaaga gggccttatg gatcactgga ggggctataa aggcaaggac
     1501 ctgtgccaga gcagccacca taaatgccgg gtgatcctgg gccagtggct tggcttctct
     1561 catgatgtcc ccactatggc tggggaggaa tggaagcatg gaggtgatca atctcccccc
     1621 aggatccata cccccaggag aagaggccta agagagaagg cctgcaaagg agacaaaaca
     1681 aaggaggcag tgagcatctt gaaacataaa ttgccaactt tcttgtacaa agttggcatt
     1741 ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa
     1801 atcattattt gccatccagc tgatatcccc tatagtgagt cgtattacat ggtcatagct
     1861 gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa
     1921 aatatatcat catgcctcct ctagaccagc caggacagaa atgcctcgac ttcgctgcta
     1981 cccaaggttg ccgggtgacg cacaccgtgg aaacggatga aggcacgaac ccagtggaca
     2041 taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg caactggtcc
     2101 agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat
     2161 gactgttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc
     2221 gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct
     2281 aaaacaaagt taaacattat gagggaagcg gtgatcgccg aagtatcgac tcaactatca
     2341 gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac
     2401 ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg
     2461 accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg
     2521 gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac
     2581 gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc
     2641 aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg
     2701 ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt
     2761 gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac
     2821 tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg
     2881 tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag
     2941 cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa
     3001 gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa
     3061 ggcgagatca ccaaggtagt cggcaaataa ccctcgagcc acccatgacc aaaatccctt
     3121 aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     3181 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     3241 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     3301 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     3361 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     3421 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     3481 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     3541 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     3601 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3661 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3721 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3781 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     3841 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     3901 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     3961 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     4021 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag
     4081 gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat
     4141 gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt
     4201 tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga
     4261 taaaacgaaa ggcccagtct tccgactgag cctttcgttt tatttgatgc ctggcagttc
     4321 cctactctcg cgttaacgct agcatggatg ttttcccagt cacgacgttg taaaacgacg
     4381 gccagtctta agctcgggcc c
//
