LOCUS       sequence-363381-        9324 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector encoding myocardin-related transcription
            factor B tagged with mTagBFP2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9324)
  AUTHORS   Zhang JZ, Nguyen AH, Miyamoto S, Heller Brown J, McCulloch AD, Zhang
            J
  TITLE     Histamine-induced biphasic activation of RhoA allows for persistent 
            RhoA signaling.
  JOURNAL   PLoS Biol. 2020 Sep 3;18(9):e3000866. doi: 
            10.1371/journal.pbio.3000866. eCollection 2020 Sep.
  PUBMED    32881857
REFERENCE   2  (bases 1 to 9324)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2020-09-3"; volume: "18(9):e3000866. doi"; pages: " 
            10.1371/journal.pbio.3000866. eCollection 2020 Sep"
COMMENT     Sequence Label: pcDNA3.1(+)-mTagBFP2-MRTF-B
FEATURES             Location/Qualifiers
     source          1..9324
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             925..1623
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     primer_bind     complement(4916..4933)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4922..5146
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5192..5619
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5279..5298)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5488..5509
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5628..5648)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5633..5962
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5813..5948
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5875..5894
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6029..6823
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6083..6102)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6693..6712
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6997..7118
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     7088..7107
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7167..7183)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7167..7183)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7180..7202)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7191..7207
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7215..7221,7222..7239,7240..7245))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7260..7281
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7398..7415)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7569..8157)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7649..7668)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8328..9119,9120..9188))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8951..8970
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9189..9293)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttgggc caccatgagc gagctgatta aggagaacat gcacatgaag
      961 ctgtacatgg agggcaccgt ggacaaccat cacttcaagt gcacatccga gggcgaaggc
     1021 aagccctacg agggcaccca gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc
     1081 ttcgccttcg acatcctggc tactagcttc ctctacggca gcaagacctt catcaaccac
     1141 acccagggca tccccgactt cttcaagcag tccttccctg agggcttcac atgggagaga
     1201 gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag cctccaggac
     1261 ggctgcctca tctacaacgt caagatcaga ggggtgaact tcacatccaa cggccctgtg
     1321 atgcagaaga aaacactcgg ctgggaggcc ttcaccgaga cgctgtaccc cgctgacggc
     1381 ggcctggaag gcagaaacga catggccctg aagctcgtgg gcgggagcca tctgatcgca
     1441 aacgccaaga ccacatatag atccaagaaa cccgctaaga acctcaagat gcctggcgtc
     1501 tactatgtgg actacagact ggaaagaatc aaggaggcca acaacgagac ctacgtcgag
     1561 cagcacgagg tggcagtggc cagatactgc gacctcccta gcaaactggg gcacaagctt
     1621 aatggaggta ctggtggaag tatgatcgat agctccaaga agcagccaca aggcttccca
     1681 gaaattttaa ctgctgagga ttttgaacct ttcaaagaaa aggaatgcct tgagggaagc
     1741 aaccagaaaa gtctcaagga agtgctccag ctgaggctgc aacaaaggag gacaagagaa
     1801 caactagtgg accagggcat catgccacct ttgaagagtc cagcagcatt ccatgagcag
     1861 ataaaaagcc tggagcgagc cagaactgag aattttctga agcacaagat ccggagccgc
     1921 ccagaccgct ctgagcttgt caggatgcat attttagaag aaacgtttgc agagccatct
     1981 cttcaggcca cacagatgaa gctgaagaga gctcggctgg cagatgacct gaacgagaag
     2041 attgctcaaa ggcctggtcc catggagctg gtggagaaga acatccttcc tgtggattcg
     2101 agtgtcaagg aagcgattat aggtgtggtg aaggaggact atcctcacac tcatggcgag
     2161 ttctcatttg atgaagatag cagtgatgct ttgtctccag accagccagc gagccaggag
     2221 tctcagggct cagctgcatc ccccagtgag ccaaaagtca gtgcctcgcc acctcctgtg
     2281 actgcaagca ctccagccca gtttacctca gtgtccccag cagttcctga attcttgaaa
     2341 acccctctaa ctgcagacca gcctcccaca aggtctacag ctcccgtgct ccctacaaac
     2401 actgtgtcct cagcgaagtc tggcccaatg ctggtgaagc aaagccatcc caagaatcca
     2461 aacgacaaac accgtagcaa aaaatgcaaa gatccgaaac cgcgagtgaa gaagcttaag
     2521 taccaccagt acattccacc caaccagaag ggggagaaga gcgagccaca gatggactcc
     2581 aactatgccc gcctgctcca gcagcagcag ctcttcctac agctgcagat cctgagccag
     2641 cagcagcagc aacagcagca gcagcactac aactaccaga ccatcctacc tgcacccatc
     2701 aaaactgaca agaacagcag cagcggcagt aacagcggca gtagcagcag catgccggcg
     2761 aggaggccag gaccgctgcc ctccagcttg gatgacttaa aggtgtcaga gctgaagaca
     2821 gaactcaaac tgaggggtct gccagtgtca ggaactaaac cagacctcat tgaacgcctg
     2881 aaaccctacc aggaagtgac cagtagtaac cttgccactg gcagtattgt ggcagtgtca
     2941 tcagcaacca ttgtcaccag taacccagag gtcacagtgg cactgccagt cacaacactg
     3001 cataatgctg taacaagctc tgtgtccact ttcaaggcag acttggcact tcctgcaacc
     3061 agcagtgtgc cccatgttga aaatgcccac tctcctctgc ccatctcccc atcaccctct
     3121 gagcagtcca gtctcagtac cgatgacaca aacatgacag acaccttcac agagatcatg
     3181 actatgatgt caccttccca gttgttgtgt tcatctcccc tgagggtggt gagccatgat
     3241 gacagcctga gcccttccag tagcaccctg tctactctag aactggatgc tgctgagaag
     3301 gaccgcaaac ttcaggagaa ggagaaacag attgaagaac tgaagaggaa actggaacaa
     3361 gaacagaagc ttgtggaggt cctcaagatg cagcttgagg tggaaaaacg tgggcagcag
     3421 cgaccaccag accctcagcc cagtgatccc ccacatccct ttaatacatc agatcctaaa
     3481 catggcagtg ttgggtcctc catcaaggat gaggcctcac tccctgactg ctccagcccc
     3541 cagcagccca tcacagtgcc tggccactct gtaggccagc ccatctccac gggtagccag
     3601 acccttgttg ccaaaaagac tgtggtcgtc aagcaagagg tccccatggc ccaagcagag
     3661 cagcagaacg ttgtctcgca gttctacctt agttcccagg gacagccacc tgcccttgtt
     3721 gctcagcctc aggccttact gaccacacag acaactcagc tgctgctgcc agtgtccatc
     3781 cagggctcaa atgttacttc agtgcaactc ccagtaggca gccttcaact ccagacccca
     3841 gcacaaggaa gagtccaggc tcagcctcat gtagctgctg ccacacaggt tccagctgca
     3901 gccctgccct cagccttgac ctcagcactg cctcagaagc aggaagcctt cccacagcac
     3961 gtgttgggcc agcctcagcc agtcagaaag gtcttcacaa actcggcacc aaacacagtt
     4021 cttcagtatc agaggcagcc tggcccaacg aaccagcagc cctttgtcag taagacctct
     4081 aaccctgctc ttcaatccag aactgcccca cttgcacccc tgcaaaatgg acccagccta
     4141 gccagcaagc ctagctcacc acccccaccc cagcagtttg ttgttcagca ctctttgttt
     4201 gcaaccccaa tcacgaagac aaaagatcca cctcgatatg aggaagccat caagcaggca
     4261 cgtagcacac agccagccct tccagaggtt tccagtgtgc acagtcagca gatggacgac
     4321 ctcttcgaca tcctcataaa gagtggagag atttccttcc caataaaaga agagccttct
     4381 ccaatttcca agatgaaacc agtgacagcc agcatcacca caatgccagt caataccgtg
     4441 gtctctcgac caccgcccca ggtccaaata gcaccacctg tatccttaga acctgtgaac
     4501 agtttatctg ccagcttaga gaaccagcta gaagccttct tggatggcac tttgccctcg
     4561 gccactgaca ctggcccact gcagaacagc agtgaagata gagagtcttt ctctctgatc
     4621 gaggatctgc agaacgactt gctgagtcat tccagcatgc tgtaccagtc tcactctccc
     4681 atggagacct ctgaggccca gttggtgtca gggaccccct gtctatctct tgacctgtca
     4741 gactccaacc tggacaacat ggagtggcta gacattacca tgcccaccac atcctccggg
     4801 ctcactccac tgagtaccac tgcgccaagc atgttctctg ctgattttct ggacccacag
     4861 gacctgccgc tgccatggga ctagtctaga gggcccgttt aaacccgctg atcagcctcg
     4921 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     4981 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     5041 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     5101 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     5161 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     5221 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     5281 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     5341 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaac
     5401 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     5461 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     5521 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     5581 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     5641 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     5701 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     5761 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     5821 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     5881 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     5941 tggaggccta ggcttttgca aaaagctccc gggagcttat atatccattt tcggatctga
     6001 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     6061 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     6121 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     6181 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
     6241 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     6301 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     6361 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     6421 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     6481 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     6541 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     6601 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     6661 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     6721 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     6781 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     6841 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
     6901 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     6961 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     7021 ggttacaaat aaagcaatag aatcacaaat ttcacaaata aagcattttt ttcactgcat
     7081 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     7141 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     7201 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     7261 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     7321 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     7381 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     7441 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     7501 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     7561 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     7621 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     7681 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     7741 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     7801 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     7861 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     7921 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     7981 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     8041 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     8101 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     8161 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     8221 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     8281 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     8341 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     8401 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     8461 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     8521 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     8581 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     8641 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     8701 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     8761 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     8821 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     8881 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     8941 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     9001 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     9061 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     9121 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     9181 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     9241 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     9301 ccccgaaaag tgccacctga cgtc
//
