LOCUS       sequence-363382-        9347 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline inducible lentiviral vector for N-terminal Flag tagged 
            KBTBD4 R313PRR expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9347)
  AUTHORS   Chen Z, Ioris RM, Richardson S, Van Ess AN, Vendrell I, Kessler BM, 
            Buffa FM, Busino L, Clifford SC, Bullock AN, D'Angiolella V
  TITLE     Disease-associated KBTBD4 mutations in medulloblastoma elicit 
            neomorphic ubiquitylation activity to promote CoREST degradation.
  JOURNAL   Cell Death Differ. 2022 Apr 4. pii: 10.1038/s41418-022-00983-4. doi:
            10.1038/s41418-022-00983-4.
  PUBMED    35379950
REFERENCE   2  (bases 1 to 9347)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Death 
            Differ. 2022 Apr 4. pii: 10.1038/s41418-022-00983-4. doi: 
            10.1038/s41418-022-00983-4."
COMMENT     Sequence Label: pCW57.1 KBTBD4 R313PRR
FEATURES             Location/Qualifiers
     source          1..9347
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        212..526
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    398..416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    434..452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     499..523
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    547..571
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             607..630
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(2220..2244)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2262..2279
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        2289..2799
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2718..2736
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2809..3405
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2809..2828)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3305..3325
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3406..3459
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(3460..4077,4078..4083,4084..4119,4120..4122,4123..4158,
                     4159..4161,4162..4197,4198..4206)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(4246..4266)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4664..4675)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4880..5113
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5185..5306
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5222..5241)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5276..5295
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5346..5481
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5481..5552
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5553..5621,5622..6413)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5771..5790)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6584..7172
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7073..7092
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7326..7343
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7460..7481
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7496..7501,7502..7519,7520..7526)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7534..7550
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7539..7561
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7558..7574
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7558..7574
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7593..7613
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7595..7613
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7641..7867
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7868..8048
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8095..8220
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8713..8946
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9131..9175
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      541 gcatcaacaa gtttgtacaa aaaagcaggc tctttaaagg aaccaattca gtcgactgga
      601 tccatggact acaaagacga tgacgacaag gaatcaccag aggagcctgg agcatccatg
      661 gatgagaact actttgtgaa ctacactttc aaagatcggt cacattcagg ccgtgtggct
      721 caaggcatca tgaaactgtg tctagaggag gagctctttg ctgatgtcac catttcggtg
      781 gaaggccggg agtttcagct ccatcggctg gtcctctcag ctcagagctg cttcttccga
      841 tccatgttca cttccaacct gaaggaggcc cacaaccggg tgattgtgct gcaggatgtc
      901 agcgagtctg ttttccagct cctggttgat tatatctacc atgggactgt gaaacttcga
      961 gctgaggagt tgcaggaaat ttatgaggtg tcagacatgt atcagctgac atctctcttt
     1021 gaggaatgct ctcggttttt ggcccgcaca gtgcaagtgg gaaactgcct tcaggtgatg
     1081 tggctggcag atcggcacag tgatcctgag ctctatacgg ctgccaagca ctgtgccaag
     1141 acccacctgg cccagctgca gaatacagag gaatttctcc acttgcccca ccgcttactc
     1201 acagatatca tctcggatgg agttccgtgt tctcagaacc caacagaggc aatagaagcc
     1261 tggatcaact ttaataaaga ggaaagagag gcttttgcag agtcactcag gacaagcttg
     1321 aaggaaattg gggagaatgt gcacatttac ctgattggga aagagtcatc tcgtacccac
     1381 tcgttggctg tgtccttgca ctgtgcagaa gatgactcca tcagtgtaag tggccaaaac
     1441 agtttgtgcc accagatcac tgcggcctgc aagcatggtg gagacttgta tgtggtggga
     1501 gggtccatcc cacggccacg gcgcatgtgg aagtgcaaca atgccaccgt tgactgggag
     1561 tggtgtgctc ctttgcctcg ggaccggctc cagcacaccc tggtgtctgt gcccgggaaa
     1621 gatgccatat attcactggg tggcaagaca ctgcaagata ccctctccaa cgcagtcatt
     1681 tattatcgcg taggtgataa tgtgtggaca gagacaactc agctagaggt ggctgtgtca
     1741 ggggctgctg gtgccaacct caacgggatc atctacttac taggggggga ggagaatgat
     1801 ctggacttct ttaccaaacc ttcccgactc atccagtgct ttgacacaga gacagacaaa
     1861 tgccatgtga agccctatgt gctgcccttt gcaggccgca tgcacgcagc tgtgcataaa
     1921 gatctggtgt tcatcgtggc tgaaggggac tccctggtgt gctacaatcc cttgctagac
     1981 agcttcaccc ggctttgcct tcctgaggcc tggagctctg ccccatccct ctggaagatt
     2041 gccagctgta acgggagcat ctatgtcttc cgggaccgat ataaaaaggg ggatgccaac
     2101 acctacaagc ttgaccctgc cacttcagcc gtaactgtca caagaggtat taaggtgctg
     2161 cttaccaatt tgcagtttgt gttggcctaa gaattcgcgg ccgcactcga gatatctaga
     2221 cccagctttc ttgtacaaag tggtttagta atgaaccggt ccaccaccac caccaccact
     2281 aaggatccgg ggttggggtt gcgccttttc caaggcagcc ctgggtttgc gcagggacgc
     2341 ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg accctgggtc tcgcacattc
     2401 ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta cccttgtggg ccccccggcg
     2461 acgcttcctg ctccgcccct aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg
     2521 tgacaaacgg aagccgcacg tctcactagt accctcgcag acggacagcg ccagggagca
     2581 atggcagcgc gccgaccgcg atgggctgtg gccaatagcg gctgctcagc agggcgcgcc
     2641 gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc
     2701 ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc
     2761 ggctccctcg ttgaccgaat caccgacctc tctccccagc aattcaccat gaccgagtac
     2821 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     2881 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     2941 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     3001 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     3061 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     3121 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     3181 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     3241 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     3301 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     3361 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgccgaagg tagaggttct
     3421 ctcctcactt gtggtgatgt tgaagaaaac cctggtccaa tgtctagact ggacaagagc
     3481 aaagtcataa acggagctct ggaattactc aatggtgtcg gtatcgaagg cctgacgaca
     3541 aggaaactcg ctcaaaagct gggagttgag cagcctaccc tgtactggca cgtgaagaac
     3601 aagcgggccc tgctcgatgc cctgccaatc gagatgctgg acaggcatca tacccacttc
     3661 tgccccctgg aaggcgagtc atggcaagac tttctgcgga acaacgccaa gtcataccgc
     3721 tgtgctctcc tctcacatcg cgacggggct aaagtgcatc tcggcacccg cccaacagag
     3781 aaacagtacg aaaccctgga aaatcagctc gcgttcctgt gtcagcaagg cttctccctg
     3841 gagaacgcac tgtacgctct gtccgccgtg ggccacttta cactgggctg cgtattggag
     3901 gaacaggagc atcaagtagc aaaagaggaa agagagacac ctaccaccga ttctatgccc
     3961 ccacttctga gacaagcaat tgagctgttc gaccggcagg gagccgaacc tgccttcctt
     4021 ttcggcctgg aactaatcat atgtggcctg gagaaacagc taaagtgcga aagcggcggg
     4081 ccgaccgacg cccttgacga ttttgactta gacatgctcc cagccgatgc ccttgacgac
     4141 tttgaccttg atatgctgcc tgctgacgct cttgacgatt ttgaccttga catgctcccc
     4201 gggtaactaa gtaaggatcg atccaagata tcgtattctt aactatgttg ctccttttac
     4261 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     4321 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     4381 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     4441 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     4501 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     4561 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
     4621 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     4681 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     4741 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctgtttcgc ctcggcgtcc
     4801 ggactagagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     4861 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     4921 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     4981 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     5041 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     5101 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     5161 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     5221 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     5281 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     5341 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     5401 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     5461 gaggaggctt ttttggaggc ctttcaaata tgtatccgct catgagacaa taaccctgat
     5521 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     5581 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     5641 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     5701 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     5761 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     5821 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     5881 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     5941 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     6001 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     6061 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     6121 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     6181 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     6241 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     6301 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     6361 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     6421 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     6481 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     6541 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     6601 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     6661 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     6721 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     6781 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     6841 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     6901 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     6961 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     7021 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     7081 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     7141 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     7201 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     7261 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     7321 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     7381 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     7441 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     7501 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     7561 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
     7621 agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt
     7681 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt
     7741 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat
     7801 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata
     7861 cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     7921 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     7981 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     8041 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     8101 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     8161 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     8221 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     8281 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     8341 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     8401 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     8461 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     8521 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     8581 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     8641 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     8701 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     8761 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     8821 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     8881 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     8941 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     9001 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     9061 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     9121 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     9181 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     9241 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     9301 atattcacca ttatcgtttc agacccacct cccaaccccg aggggac
//
