LOCUS       sequence-363385-        9344 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline inducible lentiviral vector for N-terminal Flag tagged 
            KBTBD4 P311PP expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9344)
  AUTHORS   Chen Z, Ioris RM, Richardson S, Van Ess AN, Vendrell I, Kessler BM, 
            Buffa FM, Busino L, Clifford SC, Bullock AN, D'Angiolella V
  TITLE     Disease-associated KBTBD4 mutations in medulloblastoma elicit 
            neomorphic ubiquitylation activity to promote CoREST degradation.
  JOURNAL   Cell Death Differ. 2022 Apr 4. pii: 10.1038/s41418-022-00983-4. doi:
            10.1038/s41418-022-00983-4.
  PUBMED    35379950
REFERENCE   2  (bases 1 to 9344)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Death 
            Differ. 2022 Apr 4. pii: 10.1038/s41418-022-00983-4. doi: 
            10.1038/s41418-022-00983-4."
COMMENT     Sequence Label: pCW57.1 KBTBD4 P311PP
FEATURES             Location/Qualifiers
     source          1..9344
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        212..526
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    398..416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    434..452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     499..523
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    547..571
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             607..630
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(2217..2241)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2259..2276
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        2286..2796
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2715..2733
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2806..3402
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2806..2825)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3302..3322
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3403..3456
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(3457..4074,4075..4080,4081..4116,4117..4119,4120..4155,
                     4156..4158,4159..4194,4195..4203)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(4243..4263)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4661..4672)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4877..5110
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5182..5303
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5219..5238)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5273..5292
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5343..5478
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5478..5549
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5550..5618,5619..6410)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5768..5787)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6581..7169
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7070..7089
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7323..7340
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7457..7478
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7493..7498,7499..7516,7517..7523)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7531..7547
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7536..7558
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7555..7571
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7555..7571
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7590..7610
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7592..7610
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7638..7864
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7865..8045
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8092..8217
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8710..8943
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9128..9172
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      541 gcatcaacaa gtttgtacaa aaaagcaggc tctttaaagg aaccaattca gtcgactgga
      601 tccatggact acaaagacga tgacgacaag gaatcaccag aggagcctgg agcatccatg
      661 gatgagaact actttgtgaa ctacactttc aaagatcggt cacattcagg ccgtgtggct
      721 caaggcatca tgaaactgtg tctagaggag gagctctttg ctgatgtcac catttcggtg
      781 gaaggccggg agtttcagct ccatcggctg gtcctctcag ctcagagctg cttcttccga
      841 tccatgttca cttccaacct gaaggaggcc cacaaccggg tgattgtgct gcaggatgtc
      901 agcgagtctg ttttccagct cctggttgat tatatctacc atgggactgt gaaacttcga
      961 gctgaggagt tgcaggaaat ttatgaggtg tcagacatgt atcagctgac atctctcttt
     1021 gaggaatgct ctcggttttt ggcccgcaca gtgcaagtgg gaaactgcct tcaggtgatg
     1081 tggctggcag atcggcacag tgatcctgag ctctatacgg ctgccaagca ctgtgccaag
     1141 acccacctgg cccagctgca gaatacagag gaatttctcc acttgcccca ccgcttactc
     1201 acagatatca tctcggatgg agttccgtgt tctcagaacc caacagaggc aatagaagcc
     1261 tggatcaact ttaataaaga ggaaagagag gcttttgcag agtcactcag gacaagcttg
     1321 aaggaaattg gggagaatgt gcacatttac ctgattggga aagagtcatc tcgtacccac
     1381 tcgttggctg tgtccttgca ctgtgcagaa gatgactcca tcagtgtaag tggccaaaac
     1441 agtttgtgcc accagatcac tgcggcctgc aagcatggtg gagacttgta tgtggtggga
     1501 gggtccatcc caccacggcg catgtggaag tgcaacaatg ccaccgttga ctgggagtgg
     1561 tgtgctcctt tgcctcggga ccggctccag cacaccctgg tgtctgtgcc cgggaaagat
     1621 gccatatatt cactgggtgg caagacactg caagataccc tctccaacgc agtcatttat
     1681 tatcgcgtag gtgataatgt gtggacagag acaactcagc tagaggtggc tgtgtcaggg
     1741 gctgctggtg ccaacctcaa cgggatcatc tacttactag ggggggagga gaatgatctg
     1801 gacttcttta ccaaaccttc ccgactcatc cagtgctttg acacagagac agacaaatgc
     1861 catgtgaagc cctatgtgct gccctttgca ggccgcatgc acgcagctgt gcataaagat
     1921 ctggtgttca tcgtggctga aggggactcc ctggtgtgct acaatccctt gctagacagc
     1981 ttcacccggc tttgccttcc tgaggcctgg agctctgccc catccctctg gaagattgcc
     2041 agctgtaacg ggagcatcta tgtcttccgg gaccgatata aaaaggggga tgccaacacc
     2101 tacaagcttg accctgccac ttcagccgta actgtcacaa gaggtattaa ggtgctgctt
     2161 accaatttgc agtttgtgtt ggcctaagaa ttcgcggccg cactcgagat atctagaccc
     2221 agctttcttg tacaaagtgg tttagtaatg aaccggtcca ccaccaccac caccactaag
     2281 gatccggggt tggggttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc
     2341 tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc
     2401 acgtccgttc gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg
     2461 cttcctgctc cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga
     2521 caaacggaag ccgcacgtct cactagtacc ctcgcagacg gacagcgcca gggagcaatg
     2581 gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct gctcagcagg gcgcgccgag
     2641 agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg
     2701 ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc
     2761 tccctcgttg accgaatcac cgacctctct ccccagcaat tcaccatgac cgagtacaag
     2821 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     2881 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     2941 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     3001 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     3061 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     3121 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     3181 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     3241 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     3301 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     3361 gaaggaccgc gcacctggtg catgacccgc aagcccggtg ccgaaggtag aggttctctc
     3421 ctcacttgtg gtgatgttga agaaaaccct ggtccaatgt ctagactgga caagagcaaa
     3481 gtcataaacg gagctctgga attactcaat ggtgtcggta tcgaaggcct gacgacaagg
     3541 aaactcgctc aaaagctggg agttgagcag cctaccctgt actggcacgt gaagaacaag
     3601 cgggccctgc tcgatgccct gccaatcgag atgctggaca ggcatcatac ccacttctgc
     3661 cccctggaag gcgagtcatg gcaagacttt ctgcggaaca acgccaagtc ataccgctgt
     3721 gctctcctct cacatcgcga cggggctaaa gtgcatctcg gcacccgccc aacagagaaa
     3781 cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc agcaaggctt ctccctggag
     3841 aacgcactgt acgctctgtc cgccgtgggc cactttacac tgggctgcgt attggaggaa
     3901 caggagcatc aagtagcaaa agaggaaaga gagacaccta ccaccgattc tatgccccca
     3961 cttctgagac aagcaattga gctgttcgac cggcagggag ccgaacctgc cttccttttc
     4021 ggcctggaac taatcatatg tggcctggag aaacagctaa agtgcgaaag cggcgggccg
     4081 accgacgccc ttgacgattt tgacttagac atgctcccag ccgatgccct tgacgacttt
     4141 gaccttgata tgctgcctgc tgacgctctt gacgattttg accttgacat gctccccggg
     4201 taactaagta aggatcgatc caagatatcg tattcttaac tatgttgctc cttttacgct
     4261 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     4321 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     4381 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     4441 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     4501 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     4561 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc
     4621 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     4681 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     4741 tcagacgagt cggatctccc tttgggccgc ctccccgcct gtttcgcctc ggcgtccgga
     4801 ctagaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     4861 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
     4921 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     4981 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     5041 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     5101 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     5161 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
     5221 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     5281 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
     5341 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     5401 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     5461 gaggcttttt tggaggcctt tcaaatatgt atccgctcat gagacaataa ccctgataaa
     5521 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     5581 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     5641 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     5701 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     5761 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     5821 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     5881 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     5941 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     6001 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     6061 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     6121 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     6181 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     6241 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     6301 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     6361 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     6421 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     6481 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     6541 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     6601 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     6661 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     6721 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     6781 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     6841 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     6901 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     6961 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     7021 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     7081 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     7141 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     7201 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     7261 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     7321 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     7381 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     7441 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     7501 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     7561 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
     7621 tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac
     7681 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
     7741 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg
     7801 acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat
     7861 aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     7921 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     7981 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     8041 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
     8101 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     8161 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
     8221 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
     8281 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     8341 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     8401 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     8461 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     8521 gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg
     8581 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     8641 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     8701 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     8761 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     8821 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     8881 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     8941 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     9001 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     9061 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     9121 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     9181 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     9241 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     9301 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggac
//
