LOCUS       sequence-363386-        9341 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline inducible lentiviral vector for N-terminal Flag tagged 
            KBTBD4 WT expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9341)
  AUTHORS   Chen Z, Ioris RM, Richardson S, Van Ess AN, Vendrell I, Kessler BM, 
            Buffa FM, Busino L, Clifford SC, Bullock AN, D'Angiolella V
  TITLE     Disease-associated KBTBD4 mutations in medulloblastoma elicit 
            neomorphic ubiquitylation activity to promote CoREST degradation.
  JOURNAL   Cell Death Differ. 2022 Apr 4. pii: 10.1038/s41418-022-00983-4. doi:
            10.1038/s41418-022-00983-4.
  PUBMED    35379950
REFERENCE   2  (bases 1 to 9341)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Death 
            Differ. 2022 Apr 4. pii: 10.1038/s41418-022-00983-4. doi: 
            10.1038/s41418-022-00983-4."
COMMENT     Sequence Label: pCW57.1 KBTBD4 WT
FEATURES             Location/Qualifiers
     source          1..9341
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        212..526
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    398..416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    434..452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     499..523
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    547..571
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             607..630
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(2214..2238)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2256..2273
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        2283..2793
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2712..2730
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2803..3399
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2803..2822)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3299..3319
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3400..3453
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(3454..4071,4072..4077,4078..4113,4114..4116,4117..4152,
                     4153..4155,4156..4191,4192..4200)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(4240..4260)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4658..4669)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4874..5107
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5179..5300
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5216..5235)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5270..5289
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5340..5475
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5475..5546
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5547..5615,5616..6407)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5765..5784)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6578..7166
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7067..7086
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7320..7337
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7454..7475
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7490..7495,7496..7513,7514..7520)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7528..7544
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7533..7555
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7552..7568
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7552..7568
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7587..7607
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7589..7607
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7635..7861
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7862..8042
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8089..8214
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8707..8940
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9125..9169
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      541 gcatcaacaa gtttgtacaa aaaagcaggc tctttaaagg aaccaattca gtcgactgga
      601 tccatggact acaaagacga tgacgacaag gaatcaccag aggagcctgg agcatccatg
      661 gatgagaact actttgtgaa ctacactttc aaagatcggt cacattcagg ccgtgtggct
      721 caaggcatca tgaaactgtg tctagaggag gagctctttg ctgatgtcac catttcggtg
      781 gaaggccggg agtttcagct ccatcggctg gtcctctcag ctcagagctg cttcttccga
      841 tccatgttca cttccaacct gaaggaggcc cacaaccggg tgattgtgct gcaggatgtc
      901 agcgagtctg ttttccagct cctggttgat tatatctacc atgggactgt gaaacttcga
      961 gctgaggagt tgcaggaaat ttatgaggtg tcagacatgt atcagctgac atctctcttt
     1021 gaggaatgct ctcggttttt ggcccgcaca gtgcaagtgg gaaactgcct tcaggtgatg
     1081 tggctggcag atcggcacag tgatcctgag ctctatacgg ctgccaagca ctgtgccaag
     1141 acccacctgg cccagctgca gaatacagag gaatttctcc acttgcccca ccgcttactc
     1201 acagatatca tctcggatgg agttccgtgt tctcagaacc caacagaggc aatagaagcc
     1261 tggatcaact ttaataaaga ggaaagagag gcttttgcag agtcactcag gacaagcttg
     1321 aaggaaattg gggagaatgt gcacatttac ctgattggga aagagtcatc tcgtacccac
     1381 tcgttggctg tgtccttgca ctgtgcagaa gatgactcca tcagtgtaag tggccaaaac
     1441 agtttgtgcc accagatcac tgcggcctgc aagcatggtg gagacttgta tgtggtggga
     1501 gggtccatcc cacggcgcat gtggaagtgc aacaatgcca ccgttgactg ggagtggtgt
     1561 gctcctttgc ctcgggaccg gctccagcac accctggtgt ctgtgcccgg gaaagatgcc
     1621 atatattcac tgggtggcaa gacactgcaa gataccctct ccaacgcagt catttattat
     1681 cgcgtaggtg ataatgtgtg gacagagaca actcagctag aggtggctgt gtcaggggct
     1741 gctggtgcca acctcaacgg gatcatctac ttactagggg gggaggagaa tgatctggac
     1801 ttctttacca aaccttcccg actcatccag tgctttgaca cagagacaga caaatgccat
     1861 gtgaagccct atgtgctgcc ctttgcaggc cgcatgcacg cagctgtgca taaagatctg
     1921 gtgttcatcg tggctgaagg ggactccctg gtgtgctaca atcccttgct agacagcttc
     1981 acccggcttt gccttcctga ggcctggagc tctgccccat ccctctggaa gattgccagc
     2041 tgtaacggga gcatctatgt cttccgggac cgatataaaa agggggatgc caacacctac
     2101 aagcttgacc ctgccacttc agccgtaact gtcacaagag gtattaaggt gctgcttacc
     2161 aatttgcagt ttgtgttggc ctaagaattc gcggccgcac tcgagatatc tagacccagc
     2221 tttcttgtac aaagtggttt agtaatgaac cggtccacca ccaccaccac cactaaggat
     2281 ccggggttgg ggttgcgcct tttccaaggc agccctgggt ttgcgcaggg acgcggctgc
     2341 tctgggcgtg gttccgggaa acgcagcggc gccgaccctg ggtctcgcac attcttcacg
     2401 tccgttcgca gcgtcacccg gatcttcgcc gctacccttg tgggcccccc ggcgacgctt
     2461 cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa
     2521 acggaagccg cacgtctcac tagtaccctc gcagacggac agcgccaggg agcaatggca
     2581 gcgcgccgac cgcgatgggc tgtggccaat agcggctgct cagcagggcg cgccgagagc
     2641 agcggccggg aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc
     2701 ctgcccgcgc ggtgttccgc attctgcaag cctccggagc gcacgtcggc agtcggctcc
     2761 ctcgttgacc gaatcaccga cctctctccc cagcaattca ccatgaccga gtacaagccc
     2821 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     2881 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     2941 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     3001 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     3061 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     3121 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     3181 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     3241 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     3301 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     3361 ggaccgcgca cctggtgcat gacccgcaag cccggtgccg aaggtagagg ttctctcctc
     3421 acttgtggtg atgttgaaga aaaccctggt ccaatgtcta gactggacaa gagcaaagtc
     3481 ataaacggag ctctggaatt actcaatggt gtcggtatcg aaggcctgac gacaaggaaa
     3541 ctcgctcaaa agctgggagt tgagcagcct accctgtact ggcacgtgaa gaacaagcgg
     3601 gccctgctcg atgccctgcc aatcgagatg ctggacaggc atcataccca cttctgcccc
     3661 ctggaaggcg agtcatggca agactttctg cggaacaacg ccaagtcata ccgctgtgct
     3721 ctcctctcac atcgcgacgg ggctaaagtg catctcggca cccgcccaac agagaaacag
     3781 tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac
     3841 gcactgtacg ctctgtccgc cgtgggccac tttacactgg gctgcgtatt ggaggaacag
     3901 gagcatcaag tagcaaaaga ggaaagagag acacctacca ccgattctat gcccccactt
     3961 ctgagacaag caattgagct gttcgaccgg cagggagccg aacctgcctt ccttttcggc
     4021 ctggaactaa tcatatgtgg cctggagaaa cagctaaagt gcgaaagcgg cgggccgacc
     4081 gacgcccttg acgattttga cttagacatg ctcccagccg atgcccttga cgactttgac
     4141 cttgatatgc tgcctgctga cgctcttgac gattttgacc ttgacatgct ccccgggtaa
     4201 ctaagtaagg atcgatccaa gatatcgtat tcttaactat gttgctcctt ttacgctatg
     4261 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     4321 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     4381 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     4441 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     4501 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     4561 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     4621 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     4681 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     4741 gacgagtcgg atctcccttt gggccgcctc cccgcctgtt tcgcctcggc gtccggacta
     4801 gaggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     4861 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
     4921 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     4981 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     5041 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     5101 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     5161 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     5221 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     5281 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     5341 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     5401 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     5461 gcttttttgg aggcctttca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     5521 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     5581 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     5641 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     5701 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     5761 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     5821 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     5881 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     5941 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     6001 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     6061 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     6121 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     6181 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     6241 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     6301 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     6361 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     6421 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     6481 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     6541 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     6601 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     6661 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     6721 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     6781 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     6841 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     6901 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     6961 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     7021 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     7081 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     7141 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     7201 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     7261 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     7321 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     7381 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     7441 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     7501 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     7561 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     7621 agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
     7681 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt
     7741 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
     7801 aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa
     7861 cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     7921 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     7981 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     8041 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     8101 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     8161 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     8221 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     8281 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     8341 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     8401 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     8461 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     8521 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
     8581 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     8641 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     8701 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     8761 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     8821 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     8881 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     8941 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     9001 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     9061 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     9121 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     9181 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     9241 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     9301 accattatcg tttcagaccc acctcccaac cccgagggga c
//
