LOCUS       sequence-363406-       10473 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMV and T7 promoter expression plasmid for human codon usage 
            anCas(SCA) with an N-terminal NLS, C-terminal bi-partite NLS, and 
            P2A-eGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10473)
  AUTHORS   Alonso-Lerma B, Jabalera Y, Samperio S, Morin M, Fernandez A, Hille 
            LT, Silverstein RA, Quesada-Ganuza A, Reifs A, Fernandez-Penalver S,
            Benitez Y, Soletto L, Gavira JA, Diaz A, Vranken W, Sanchez-Mejias 
            A, Guell M, Mojica FJM, Kleinstiver BP, Moreno-Pelayo MA, Montoliu 
            L, Perez-Jimenez R
  TITLE     Evolution of CRISPR-associated endonucleases as inferred from 
            resurrected proteins.
  JOURNAL   Nat Microbiol. 2023 Jan;8(1):77-90. doi: 10.1038/s41564-022-01265-y.
            Epub 2023 Jan 2.
  PUBMED    36593295
REFERENCE   2  (bases 1 to 10473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-022-01265-y"; journalName: "Nat Microbiol"; date: 
            "2023-01"; volume: "8"; issue: "1"; pages: "77-90"
COMMENT     Sequence Label: pCMV-T7-NLS(SV40)-anCas(SCA)-BPNLS(SV40)-P2A-E...
FEATURES             Location/Qualifiers
     source          1..10473
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(86..105)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        277..656
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        657..860
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     811..831
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     905..924
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        905..923
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             952..972
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5128..5148
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5167..5223
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(5224..5226,5227..5229,5230..5943)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5224..5226,5227..5229,5230..5940)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     5877..5898
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5977..5997
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6106..6123)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6112..6336
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6382..6810
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6469..6488)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6679..6700
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6819..6839)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6824..7153
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7004..7139
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7066..7085
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7220..8014
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7274..7293)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7884..7903
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8188..8309
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8225..8244)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8279..8298
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8358..8374)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8358..8374)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8371..8393)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8382..8398
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8406..8412,8413..8430,8431..8436))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8451..8472
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8589..8606)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8760..9348)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8840..8859)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9519..10310,10311..10379))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10142..10161
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(10380..10473,1..11))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc
       61 ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
      121 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
      181 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
      241 cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt
      301 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      361 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      421 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      481 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      541 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      601 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      661 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      721 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      781 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      841 aggtctatat aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg
      901 aaattaatac gactcactat agggagaccc aagctggcta gcgccaccat gcccaagaaa
      961 aagcggaagg tgcccaagat gaagaagccc tacagcatcg gcctggacat cggcaccaac
     1021 agcgtgggct gggccgtgat caccgacgac tacaaggtgc ccgccaagaa gatgaaggtg
     1081 ctgggcaaca ccgacaagca gagcatcaag aagaacctgc tgggcgccct gctgttcgac
     1141 agcggcgaga ccgccgaggc caccaggctg aagaggaccg ccaggaggag gtacaccagg
     1201 aggaggaaca ggatctgcta cctgcaggag atcttcagcg aggagatgaa caaggtggac
     1261 gacagcttct tccacaggct ggacgagagc ttcctggtgc ccgaggacaa gaagtacgac
     1321 aggcacccca tcttcggcaa cctggccgag gaggtggcct accacgagca gtaccccacc
     1381 atctaccacc tgaggaagca cctggccgac agcaccgaga aggccgacct gaggctggtg
     1441 tacctggccc tggcccacat catcaagttc aggggccact tcctgatcga gggcgacctg
     1501 aacgccgaga acaccgacgt gcagaagctg ttccagcagt tcgtggaggt gtacaaccag
     1561 accttcgagg agagccacct gagcgaggag accatcgacg tggaggagat cctgaccgag
     1621 aagatcagca agagcaggag gctggagaac ctgatcaagc acttccccaa cgagaagaag
     1681 aacggcctgt tcggcaacct gatcgccctg atcctgggcc tgcagcccaa cttcaagacc
     1741 aacttcgacc tgagcgagga cgccaagctg cagttcagca aggacaccta cgaggaggac
     1801 ctggagaacc tgctggccca gatcggcgac gagtacgccg acctgttcct ggccgccaag
     1861 aacctgtacg acgccatcct gctgagcggc atcctgaccg tgaccgacaa cagcaccaag
     1921 gcccccctga gcgccagcat gatcaagagg tacgaggagc accagaagga cctggcccag
     1981 ctgaagcagt tcatcaagga gcacctgccc gacaagtaca acgagatctt cagcgacaag
     2041 agcaagaacg gctacgccgg ctacatcgag ggcaagacca gccaggagga cttctacaag
     2101 tacctgaaga agatcctgag caagatcgac ggcgccgagt acttcctgga caagatcgac
     2161 agggaggact tcctgaggaa gcagaggacc ttcgacaacg gcagcatccc ccaccagatc
     2221 cacctgcagg agctgcacgc catcctgagg aggcagggcg agtactaccc cttcctgaag
     2281 gagaaccagg agaagatcga gaagatcctg accttcagga tcccctacta cgtgggcccc
     2341 ctggccaggg gcaacagcag gttcgcctgg ctgaccagga agagcgacga gaagatcacc
     2401 ccctggaact tcgacgaggt ggtggacaag gagagcagcg ccgaggcctt catcgagagg
     2461 atgaccaact acgacaccta cctgcccaac gagaaggtgc tgcccaagca cagcctgctg
     2521 tacgagacct tcaccgtgta caacgagctg accaaggtga agtacgtgac cgagaggggc
     2581 aagacccagc agttcttcag cgccgagcag aagcaggaga tcttcgacca cctgttcaag
     2641 aagaacagga aggtgaccaa gaagaagctg aaggactacc tggagaagga gttcgaggag
     2701 ttcgacagcg tggacatcac cggcgtggag gacgagttca acgccagcct gggcacctac
     2761 cacgacctgc tgaagatcct gaaggacaag gacttcctgg acgacgagga gaacgaggag
     2821 atcctggagg acatcgtgct gaccctgacc ctgttcgagg acagggagat gatcaggaag
     2881 aggctggaga agtacagcga cctgttcacc aaggagcagc tgaagaagct ggagaggagg
     2941 cactacaccg gctggggcag gctgagcagg aagctgatca acggcatcag ggacaagcag
     3001 agcggcaaga ccatcctgga ctacctgatc gacgacggca gcagcaacag gaacttcatg
     3061 cagctgatca acgacgacag cctgagcttc aaggaggaga tcgagaaggc ccaggtgatc
     3121 ggcgagaccg acaacctgca cgaggtggtg caggacctgg ccggcagccc cgccatcaag
     3181 aagggcatcc tgcagagcct gaagatcgtg gacgagctgg tgaaggtgat gggcaggcac
     3241 aaccccgaga acatcgtggt ggagatggcc agggagaacc agaccaccaa caggggcagg
     3301 aggaacagca ggcagaggct gaagaggctg gaggaggccc tgaagaacct gggcagcaac
     3361 atcctgaagg agcaccccgt ggacaaccag cagctgcaga acgacaggct gtacctgtac
     3421 tacctgcaga acggcaagga catgtacacc ggcgaggagc tggacatcga caacctgagc
     3481 cagtacgaca tcgaccacat catcccccag agcttcatca aggacaacag catcgacaac
     3541 agggtgctga ccagcagcga ggagaacagg ggcaagagcg acaacgtgcc cagcatcgag
     3601 gtggtgagga agatgaagag cttctggcag aagctgctga acgccggcct gatcagccag
     3661 aggaagttcg acaacctgac caaggccgag aggggcggcc tgaccgagga cgacaaggcc
     3721 ggcttcatca agaggcagct ggtggagacc aggcagatca ccaagcacgt ggcccagatc
     3781 ctggacagca ggttcaacac cgagagggac gagaacgaca agaggatcag gaacgtgaag
     3841 atcatcaccc tgaagagcaa cctggtgagc cagttcagga aggacttcgg cctgtacaag
     3901 gtgagggaga tcaacgacta ccaccacgcc cacgacgcct acctgaacgc cgtggtgggc
     3961 accgccctgc tgaagaagta ccccaagctg gagcccgagt tcgtgtacgg cgactacaag
     4021 aagtacaacc tgaggaagct gatcgccaag agcgacaggg agaggggcaa ggccaccgcc
     4081 aagatgttct tctacagcaa catcatgaac ttcttcaaga ccgaggtgaa gctggccgac
     4141 ggcaccgtga tcgagaggcc cgtgatcgag accaacaagg agaccggcga gatcgtgtgg
     4201 gacaaggaga aggacttcgc caccgtgagg aaggtgctga gctaccccca ggtgaacatc
     4261 gtgaagaagg tggaggtgca gaccggcggc ttcagcaagg agagcatcct gcccaagggc
     4321 aacagcgaca agctgatccc caggaagaac aactgggacc ccaagaagta cggcggcttc
     4381 gacagcccca ccgtggccta cagcgtgctg gtggtggccg acgtggagaa gggcaaggcc
     4441 aagaagctga agaccgtgaa ggagctggtg ggcatcacca tcatggagag gagcgccttc
     4501 gagaagaacc ccatcgcctt cctggagaag aagggctacc agaacatcca ggaggacctg
     4561 atcatcaagc tgcccaagta cagcctgttc gagctggaga acggcaggag gaggctgctg
     4621 gccagcgcca aggagctgca gaagggcaac gagatggtgc tgcccgccca cctggtgacc
     4681 ctgctgtacc acgccaagag gatcgacaag agcaacggca acagcgagaa cctggagcag
     4741 aagcaggagt acgtggagaa gcacaagaac gagttcgacg agctgctgga ctacatcatc
     4801 gacttcagcg agaagtacat cctggccgac aagaacctgg agaagatcaa ggagctgtac
     4861 gaccagaacg acgacgccga catcaacgag ctggccagca gcttcatcaa cctgctgacc
     4921 ttcaccgccc tgggcgcccc cgccgccttc aagttcttcg gcgagaccat cgacaggaag
     4981 aggtacacca gcaccaagga ggtgctgaac gccaccctga tccaccagag catcaccggc
     5041 ctgtacgaga ccaggatcga cctgagcaag ctgggcgagg accactctgg cggctcaaaa
     5101 agaaccgccg acggcagcga attcgagccc aagaagaaga ggaaagtcgg aggcggtgga
     5161 agcggcgcaa caaacttctc tctgctgaaa caagccggag atgtcgaaga gaatcctgga
     5221 ccgatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     5281 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     5341 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     5401 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
     5461 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     5521 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     5581 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     5641 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     5701 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     5761 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     5821 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     5881 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     5941 tgacatcatc atcaccacgc ctctggaaca ggcggaccta aaaagaagag gaaggtttaa
     6001 ggtaccgagc tcggatccac tagtccagtg tggtggaatt ctgcagatat ccagcacagt
     6061 ggcggccgct cgagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt
     6121 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     6181 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     6241 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     6301 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     6361 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     6421 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     6481 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     6541 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     6601 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     6661 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     6721 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     6781 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
     6841 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     6901 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     6961 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     7021 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     7081 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     7141 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac
     7201 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     7261 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     7321 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     7381 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
     7441 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     7501 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     7561 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     7621 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     7681 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     7741 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     7801 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     7861 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     7921 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     7981 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
     8041 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
     8101 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     8161 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     8221 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     8281 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     8341 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     8401 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     8461 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     8521 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     8581 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     8641 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     8701 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     8761 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     8821 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     8881 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     8941 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     9001 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     9061 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     9121 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     9181 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     9241 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     9301 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     9361 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     9421 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     9481 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     9541 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     9601 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     9661 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     9721 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     9781 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     9841 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     9901 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     9961 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
    10021 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
    10081 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
    10141 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
    10201 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
    10261 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
    10321 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
    10381 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
    10441 atatttgaat gtatttagaa aaataaacaa ata
//
