LOCUS       sequence-363409-        9511 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral transfer plasmid for expression of ArgSen (-)NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9511)
  AUTHORS   Rogers LC, Zhou J, Baker A, Schutt CR, Panda PK, Van Tine BA
  TITLE     Intracellular arginine-dependent translation sensor reveals the 
            dynamics of arginine starvation response and resistance in 
            ASS1-negative cells.
  JOURNAL   Cancer Metab. 2021 Jan 21;9(1):4. doi: 10.1186/s40170-021-00238-9.
  PUBMED    33478587
REFERENCE   2  (bases 1 to 9511)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Metab."; date: "2021-01-21"; volume: "9(1):4. doi"; pages: " 
            10.1186/s40170-021-00238-9"
COMMENT     Sequence Label: pKLV2-EF1a-ArgSen (-)NLS
FEATURES             Location/Qualifiers
     source          1..9511
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        960..1158
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1113..1133
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1176..1356
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1403..1528
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2027..2260
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2445..2489
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2787..2904
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3375..4556
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3606..4548
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4505..4525
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             5350..5406
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(6186..6206)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6604..6615)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7067..7084)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7073..7297
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7323..7343)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7323..7341)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7362..7378)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7362..7378)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7375..7397)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7386..7402
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7410..7416,7417..7434,7435..7440))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7455..7476
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7593..7610)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7764..8352)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7844..7863)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8523..9314,9315..9383))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9146..9165
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9384..9488)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgactagtac
      661 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      721 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      781 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      841 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      901 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      961 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
     1021 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
     1081 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
     1141 ggaggtctat ataagcagcg cgttttgcct gtactgggtc tctctggtta gaccagatct
     1201 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     1261 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     1321 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     1381 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     1441 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     1501 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     1561 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     1621 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     1681 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     1741 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     1801 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     1861 gcaagcggcc ggccgcgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     1921 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     1981 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     2041 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     2101 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     2161 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     2221 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     2281 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     2341 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     2401 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     2461 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     2521 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     2581 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     2641 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     2701 gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg ccaattctgc
     2761 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
     2821 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     2881 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg
     2941 ttagtaccgg gccctacgcg tgaggggcgc ggaagccggg gcggaggagg agccaacccc
     3001 gggctgggct gagacccgca gaggaagacg ctctagggat ttgtcccgga ctagcgagat
     3061 ggcaaggctg aggacgggag gctgattgag aggcgaaggt acaccctaat ctcaatacaa
     3121 cctttggagc taagccagca atggtagagg gaagattctg cacgtccctt ccaggcggcc
     3181 tccccgtcac cacccccccc aacccgcccc gaccggagct gagagtaatt catacaaaag
     3241 gactcgcccc tgccttgggg aatcccaggg accgtcgtta aactcccact aacgtagaac
     3301 ccagagatcg ctgcgttccc gccccctcac ccgcccgctc tcgtcatcac tgaggtggag
     3361 aagagcatgc gtgaggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc
     3421 ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc ctagagaaag tggcgcgggg
     3481 taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac
     3541 cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa
     3601 cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt
     3661 gcgtgccttg aattacttcc acgcccctgg ctgcagtacg tgattcttga tcccgagctt
     3721 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt
     3781 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt
     3841 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct
     3901 gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt
     3961 atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg
     4021 gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc
     4081 cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg
     4141 ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca
     4201 gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa
     4261 aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg
     4321 ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg
     4381 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca
     4441 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc
     4501 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtggaag
     4561 cttggcgcgc ccaccatggc gcctgttgct gggtccgaac tcccccgacg ccctctccct
     4621 ccagcggcgc aggaaaggga cgcagaacct cgcccccccc acgggggact tggtaaggac
     4681 cccaatgatc ccacagctga cgtagagatc aacccggact tgaaaccgat aatcgggcga
     4741 gacagcggaa gggcgttggc ggaagtcctg aaaaaaccag tggcttcccg acagcttcaa
     4801 ggcatgacca atgggcgaac gtcttcaagg ggacaactgc tgaaagagtt tcagctgaaa
     4861 ccaattggaa agaagatgag ctccgctgtt ggctttgtgc ccgataccgg ccctatgcat
     4921 gctagcatgc aagttacttt gaaaactctc cagcaacaga cctttaagat agatatagat
     4981 ccagaggaaa cagtaaaggc gttgaaagaa aaaattgaaa gtgagaaagg caaagacgct
     5041 tttccagttg ctgggcagaa actcatatac gccggaaaga tcctcaacga cgacacggct
     5101 ttgaaagagt ataaaataga cgagaagaat ttcgtggtcg tcatggtgac aaagccgaaa
     5161 gcattcgaat ctagaggatc agcgggttca gcggcaggta gcggcgagtt ccgcgaaagg
     5221 tgccggccac gggaccgcta taggccacgc gaaagatgca ggtgtaggga ccggatgcgg
     5281 gcgagggagc ggacgagaca acgggacagg tgtaggtcac gagagaggga attcgtcgac
     5341 ggaagcggag ctactaactt cagcctgctg aagcaggctg gcgacgtgga ggagaaccct
     5401 ggacctgtcg acggatccat gagtaagggc gaggaggata acatggctat tattaaagaa
     5461 tttatgagat tcaaagttca cgtggaaggg tccgtgaatg gtcacgaatt tgagatcgaa
     5521 ggtgaaggaa agggaagacc ctacgaaggt actcagacag ctaagttgaa ggtaacgaag
     5581 ggtggtcccc tcccgtttgc ttgggatatc ctcagccccc aatttatgta cggtagccgc
     5641 gcttatgtta aacatccggc ggacatacca gattactgga agctgagctt cccggagggg
     5701 tttaagtggg agagggttat gaacttcgag gatggaggtg tggttaccgt gacccaagac
     5761 agtagcctgc aggatggaga atttatatat aaggtgaaac tcagaggcac caactttccc
     5821 tctgacggcc cagtgatgca gaagaaaacg atgggatggg aggcatcaac cgaacggatg
     5881 taccctgaag atggggcttt gaagggtgaa ataaaacaac ggttgaagct taaagatggg
     5941 ggccactacg atgccgaggt aaagacgact tataaggcaa aaaaacccgt acaacttcca
     6001 ggggcgtaca acgtaaatat aaaattggac ataacttcac acaatgaaga ttatactatt
     6061 gtcgagcagt acgaacggag cgaaggacgg cattctactg gcggtatgga tgagctttac
     6121 aagtaagcgg ccgctcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     6181 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     6241 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     6301 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     6361 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     6421 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     6481 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     6541 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     6601 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     6661 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     6721 caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     6781 gaaaaggggg gactggaagg gctaattcac tcccaaagaa gtcaagatct gctttttgcc
     6841 tgtactgggt ctctctggtt agaccagagt ctctctggtt agaccagatc tgagcctggg
     6901 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     6961 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     7021 tttagtcagt gtggaaaatc tctagcagtt taaacccgct gatcagcctc gactgtgcct
     7081 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     7141 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     7201 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagtc
     7261 aatagcaggc atgctgggga tgcggtgggc tctatgggcg gccgttaatt aagcttttgt
     7321 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
     7381 tgaaattgtt atccgctcac aattccacac aacatacgag ccgggagcat aaagtgtaaa
     7441 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     7501 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     7561 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     7621 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     7681 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     7741 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     7801 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     7861 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     7921 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     7981 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     8041 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     8101 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     8161 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     8221 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     8281 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     8341 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     8401 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     8461 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     8521 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     8581 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     8641 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     8701 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     8761 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     8821 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     8881 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     8941 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     9001 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     9061 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     9121 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     9181 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     9241 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     9301 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     9361 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     9421 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     9481 gttccgcgca catttccccg aaaagtgcca c
//
