LOCUS       sequence-363423-       11322 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid expressing a biosensor for acetic acid and pHluorin: 
            BM3R1-Haa1-mTurquoise2 under RET2p, mCherry under HREsYGP1p and 
            sfpHluorin under TDH3p with flanks for genome integration into HO 
            locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11322)
  AUTHORS   Mormino M, Lenitz I, Siewers V, Nygard Y
  TITLE     Identification of acetic acid sensitive strains through 
            biosensor-based screening of a Saccharomyces cerevisiae CRISPRi 
            library.
  JOURNAL   Microb Cell Fact. 2022 Oct 15;21(1):214. doi: 
            10.1186/s12934-022-01938-7.
  PUBMED    36243715
REFERENCE   2  (bases 1 to 11322)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2022-10-15"; volume: "21(1):214. doi"; pages: " 
            10.1186/s12934-022-01938-7"
COMMENT     Sequence Label: pMM4_14L
FEATURES             Location/Qualifiers
     source          1..11322
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(44..859)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(44..859)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     767..786
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     851..870
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      1084..1672
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1573..1592
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             7173..7883
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVDGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7318..7336)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7586..7605
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        8168..8834
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     8704..8727
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             8841..9554
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNEHLVYITADKQKNGTKA
                     IFQVHHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLHTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     9494..9514
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      9570..9757
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(9625..9649)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     terminator      10605..10802
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 tgccccgagc ggccgcgtgt tacaaccaat taaccaattc tgattagaaa aactcatcga
       61 gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa
      121 gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct
      181 ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt
      241 caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg
      301 gcaaaagctt atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat
      361 caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgaggcgaa
      421 atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga
      481 acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga
      541 atgctgtttt cccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa
      601 aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat
      661 ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg
      721 gcttcccata caatcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt
      781 tatacccata taaatcagca tccatgttgg aatttaatcg cggcctggag caagacgttt
      841 cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta
      901 ttgttcatga tgatatattt ttatcttgtg caatgtaaca tcagagattt tgagacacaa
      961 cgtggctttg ttgaataaat cgaacttttg ctgagttgaa ggatcagtca tgaccaaaat
     1021 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     1081 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     1141 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     1201 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     1261 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     1321 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     1381 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     1441 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     1501 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     1561 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     1621 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     1681 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     1741 tgcgttatcc cctgattctg tggataaccg tgcggccgcc aattctaatt gtatcgagat
     1801 cacttttcgt gatccgctaa tcagcgacgg tcacattagg tttgccaagt cagggtatga
     1861 accatacgat cagttttcgt gaacctggta cgtatattgt ggcgtttgtg tatattttca
     1921 ttctttgaca acaatcaata ccaacctcaa ataggaaaag taataagttt ggcgttacac
     1981 cccaaaagac gccaaacgga tcgaacttac tcaatagcaa ttagcgagac aaaacctacg
     2041 ttaagacctg taaccgattt atcaaagcac tctgcggttc tttcttggga atattacctg
     2101 gacattttgt gccctcaaga aacgaggctc tacgagcctg ttggagcccc tcagacatta
     2161 gccgccacga atcaaacttt ttacgcgatt cggcccaaat cagtttctca cagatcattc
     2221 gtagagtgaa aaagcacatc gattatttga tacccctttg ggttaattac tgttgaggtc
     2281 tttgccgtaa gttccatcgc tgtccctgaa ttcgcatcta gactgaactg gccgataatt
     2341 gcagacgaac gacgatggct tcttatctca cttcaatagt actttccacc ggttatactt
     2401 ccggcttttc cctattaata caagctacaa tttcaatggg tggcaaataa tgtgtagaat
     2461 agaaaataag ccgacagggt aataaagaaa atttttagaa aaaaaaggtt agatggctta
     2521 tttaagttac aggctagcga aaaaaggaac ttcagggcaa gtaaagtgtt tgattgggca
     2581 ctagcatggc ttataaaggc gagcaattgt cgaaactaat taatgttgta cggactattg
     2641 ctgtcatctc gtggtaaatg cgtgttccag gtcgaatact acttgcacac aggcgagcgg
     2701 ggccccataa aagtgttgcc gatttgttaa gttgtctttt cggtttttct actctgttat
     2761 tccttacttc cctttttaag aactcttttt atccttcatt taggatcttg cacgtttccg
     2821 cctcatcact tgaattaaaa catgtctctg tcagtaaacc ttggcgtttc tattgttctt
     2881 catagttcaa cttttattat tacccgccct gcgcgtttac atttttccag caacagccag
     2941 cgaaaaatta gaaaatctgg ttgttgacac ctcaagaaca agggcaatta gcctcagcgt
     3001 cgaatataga tcatattaga atacctatag ctccatcaaa agaaatacac aagatctatg
     3061 gaatctactc ctactaagca aaaagccatc ttctctgcct ccttgttgtt gttcgctgag
     3121 agaggtttcg acgctaccac tatgccaatg attgccgaaa acgctaaggt tggtgctggt
     3181 actatctaca gatacttcaa gaacaaagaa tccttagtta atgagttgtt tcaacaacac
     3241 gtcaatgaat ttttgcaatg tatcgaatct ggtttggcta acgaaagaga tggttacagg
     3301 gacggtttcc accacatctt cgaaggtatg gtcaccttca ccaagaacca tcctagagcc
     3361 ttaggtttca tcaagaccca ctctcaaggt acttttttga ccgaagaatc cagattggct
     3421 tatcaaaagt tggtcgaatt tgtctgtact ttcttcagag aaggtcaaaa gcaaggtgtc
     3481 atcagaaatt tgccagaaaa cgctttgatt gctatcttgt tcggttcttt catggaagtc
     3541 tacgaaatga ttgaaaacga ttatttgtct ttgactgatg aattattaac cggtgttgag
     3601 gaatccttgt gggctgcctt gtctcgtcaa tctggttcta tggtcttgat aaatggcata
     3661 aagtatgcct gtgagaggtg cataagaggc catagagtaa caacatgcaa tcatacagat
     3721 caaccgctta tgatgatcaa acccaaaggt agaccttcca ctacatgcga ctattgtaaa
     3781 caacttcgaa aaaacaagaa tgcaaatcct gaaggtgttt gcacgtgtgg ccggctagag
     3841 aagaaaaaac tggcacagaa agccaaagaa gaagcaagag ctaaagccaa agaaaaacaa
     3901 agaaaacagt gtacctgcgg gactgatgag gtttgcaaat atcatgctca aaagagacat
     3961 ctaagaaagt ccccttcaag ttctcaaaag aaaggaagat ccatttctcg ttctcaacca
     4021 atgtttgaaa gggtattgtc ttctacttca cttgacagca atatgttatc cggccacgga
     4081 gcactatcag atacctctag catactgacg agcacatttt tagacagtga gccgggtgtt
     4141 ggtaaaattt caaaagatta ccatcatgtc ccttcattgg cctccatttc atccttacaa
     4201 tcctcgcaat cgttagatca aaatttcagt ataccacaaa gcccgccgtt atcttcaatg
     4261 tcatttaatt ttctcacggg aaatatcaat gaaaccaacc aaaatcacag taatcatcag
     4321 cattcaaaat caggcaataa ctggcaagat agttcggtaa gcttgccagc gaaagctgat
     4381 tcacgtctta acatgatgga taaaaacaac tctgtgggtc ttgacctatt aggccattca
     4441 aaacgaatat cgccgatatc aaactctcgt gtgggcgaag ttagcgttcc gctagaagaa
     4501 tatattcctt ctgacattga tggggttgga agagttactg ataaaagctc tttggtctac
     4561 gattggccat ttgatgaaag tattgagaga aatttcagta caaccgcaac cgctgcaact
     4621 ggtgaaagta agttcgacat taacgacaac tgtaatagaa ttaatagcaa aagttatagt
     4681 aagactaata gtatgaatgg aaacggtatg aacaatagca ataataataa tatcaacagt
     4741 aatggcaacg acaagaacaa taacaactct tctagacaag aacatcaagg aaatggacta
     4801 tttgacatgt ttacagattc atcgtcgatt tcaacgcttt cccgtgcaaa cttattattg
     4861 caagaaaaaa ttggttcgca agaaaactct gtcaaacaag aaaactattc gaaaaatcct
     4921 caacttcgtc atcaattaac ttccagaagt agatcattta ttcatcatcc ggcaaacgag
     4981 tatttgaaga atacttttgg aaattcacat agtaatgaca tcggaaaggg agttgaagtg
     5041 ctatctttga caccgagttt tatggatatt cccgaaaaag aaagagaaac ggaaagatcg
     5101 ccatcatcca attacattac tgacagacct ttcactcgaa aacctagatc ttctagcatt
     5161 gacgtaaacc ataggtatcc acctatggca ccaacaaccg tagcgacatc tcccggtgca
     5221 ttgaacaatg ccgtagcaag caatctcgac gatcaactga gtttaacatc actaaactct
     5281 cagccatcat cgatagcaaa tatgatgatg gacccttcaa acctagctga gcaaagttct
     5341 attcattcag ttcctcagtc aataaactct ccgagaatgc ctaaaactgg aagtcgccaa
     5401 gacaagaaca ttcacactaa gaaggaagaa agaaatccgc taaataacat acacgatctg
     5461 tcacaattgg aaaatgtacc agacgagatg aaccaaatgt tctccccacc attaaaaagt
     5521 atgaatagac cggatgccat aagggaaaat tcatctagta gtaatttcat aatccaagga
     5581 aatagcatga tctctacgcc ttccggaagg aatgaccttc cagatacctc tccaatgagt
     5641 agtattcaaa cagcgtcacc accaagtcaa ttactgaccg atcaaggatt tgcggatttg
     5701 gataatttca tgtcttcgtt aggttctgtt tctaaaggtg aagaattatt cactggtgtt
     5761 gtcccaattt tggttgaatt agatggtgat gttaatggtc acaaattttc tgtctccggt
     5821 gaaggtgaag gtgatgctac ttacggtaaa ttgaccttaa aatttatttg tactactggt
     5881 aaattgccag ttccatggcc aaccttagtc actactttat cttggggtgt tcaatgtttt
     5941 gcaagatacc cagatcatat gaaacaacat gactttttca agtctgccat gccagaaggt
     6001 tatgttcaag aaagaactat ttttttcaaa gatgacggta actacaagac cagagctgaa
     6061 gtcaagtttg aaggtgatac cttagttaat agaatcgaat taaaaggtat tgattttaaa
     6121 gaagatggta acattttagg tcacaaattg gaatacaatt atttctctga caatgtttac
     6181 atcactgctg acaaacaaaa gaatggtatc aaagctaact tcaaaattag acacaacatt
     6241 gaagatggtg gtgttcaatt agctgaccat tatcaacaaa atactccaat tggtgatggt
     6301 ccagtcttgt taccagacaa ccattactta tccactcaat ctaagttatc caaagatcca
     6361 aacgaaaaga gggaccacat ggtcttgtta gaatttgtta ctgctgctgg tattaccttg
     6421 ggtatggatg aattgtacaa aggatcctaa ctcgagagct tttgattaag ccttctagtc
     6481 caaaaaacac gtttttttgt catttatttc attttcttag aatagtttag tttattcatt
     6541 ttatagtcac gaatgtttta tgattctata tagggttgca aacaagcatt tttcatttta
     6601 tgttaaaaca atttcaggtt taccttttat tctgcttgtg gtgacgcgtg tatccgcccg
     6661 ctcttttggt cacccatgta tgctgccaaa ccagatgtca acacagctac aacggctagt
     6721 cggaatgaac attcattccg tgacctagga tgtgacggaa tgaaggttca ttccggactc
     6781 tagataagca cggaatgaac tttcattccg ctgaagcttg tcaatcggaa tgaaggttca
     6841 ttccggctag tcggaatgaa cattcattcc gcgacctagg atgtgacgga atgaaggttc
     6901 attccggact ctagataagc acggaatgaa ctttcattcc gctgaagctt gtcaatcgga
     6961 atgaaggttc attccggcta tctccccgga aactgtggcc ttttctggca cacatgatct
     7021 ccacgatttc aacatataaa tagcttttga taatggcaat attaatcaaa tttattttac
     7081 ttctttcttg taacatctct cttgtaatcc cttattcctt ctagctattt ttcataaaaa
     7141 accaagcaac tgcttatcaa cacacaaaca ctatggtgag caagggcgag gaggataaca
     7201 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtggacggcc
     7261 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
     7321 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt
     7381 tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc
     7441 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg
     7501 tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc
     7561 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagactatg ggctgggagg
     7621 cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc
     7681 tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga
     7741 agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca
     7801 acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg
     7861 gcatggacga gctgtacaag taaatcctaa ctcgaggcca attggtgcgg caattgataa
     7921 taacgaaaat gtcttttaat gatctgggta taatgaggaa ttttccgaac gtttttactt
     7981 tatatatata tatacatgta acatatattc tatacgctat agagaaagga aatttttcaa
     8041 ttaaaaaaaa aatagagaaa gagtttcact tcttgattat cgctaacact aatggttgaa
     8101 gtactgctac tttaatttta tgctggatgc acacactggc ttaagatgac aacgcagttc
     8161 gagtttatca ttatcaatac tgccatttca aagaatacgt aaataattaa tagtagtgat
     8221 tttcctaact ttatttagtc aaaaaattag ccttttaatt ctgctgtaac ccgtacatgc
     8281 ccaaaatagg gggcgggtta cacagaatat ataacatcgt aggtgtctgg gtgaacagtt
     8341 tattcctggc atccactaaa tataatggag cccgcttttt aagctggcat ccagaaaaaa
     8401 aaagaatccc agcaccaaaa tattgttttc ttcaccaacc atcagttcat aggtccattc
     8461 tcttagcgca actacagaga acaggggcac aaacaggcaa aaaacgggca caacctcaat
     8521 ggagtgatgc aacctgcctg gagtaaatga tgacacaagg caattgaccc acgcatgtat
     8581 ctatctcatt ttcttacacc ttctattacc ttctgctctc tctgatttgg aaaaagctga
     8641 aaaaaaaggt tgaaaccagt tccctgaaat tattccccta cttgactaat aagtatataa
     8701 agacggtagg tattgattgt aattctgtaa atctatttct taaacttctt aaattctact
     8761 tttatagtta gtcttttttt tagttttaaa acaccaagaa cttagtttcg aataaacaca
     8821 cataaacaaa caaaagatct atgagcaaag gagaagaact tttcactgga gttgtcccaa
     8881 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtccgt ggagagggtg
     8941 aaggtgatgc tacaaacgga aaactcaccc ttaaatttat ttgcactact ggaaaactac
     9001 ctgttccgtg gccaacactt gtcactactc tgtcttatgg tgttcaatgc ttttcccgtt
     9061 atccggatca catgaaacgg catgactttt tcaagagtgc catgcccgaa ggttatgtac
     9121 aggaacgcac tatatctttc aaagatgacg ggacctacaa gacgcgtgct gaagtcaagt
     9181 ttgaaggtga tacccttgtt aatcgtatcg agttaaaggg tattgatttt aaagaagatg
     9241 gaaacattct tggacacaaa ctcgagtaca actttaacga gcacttggta tacatcacgg
     9301 cagacaaaca aaagaatgga accaaagcta tcttccaagt tcaccacaac gttgaagatg
     9361 gttccgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc
     9421 ttttaccaga caaccattac ctgcacacac aatctgtcct ttcgaaagat cccaacgaaa
     9481 agcgtgacca catggtcctt cttgagtttg taactgctgc tgggattaca catggcatgg
     9541 atgagctcta caaaggatcc taactcgagg cgaatttctt atgatttatg atttttatta
     9601 ttaaataagt tataaaaaaa ataagtgtat acaaatttta aagtgactct taggttttaa
     9661 aacgaaaatt cttattcttg agtaactctt tcctgtaggt caggttgctt tctcaggtat
     9721 agcatgaggt cgctcttatt gaccacacct ctaccggcat gccgagcaaa tgcctgcaaa
     9781 tcgctcccca tttcgctgag cagttacaga gatgttacga accactagtg cactgcagta
     9841 cagtttagct tgcctcgtcc ccgccgggtc acccggccag cgacatggag gcccagaata
     9901 ccctccttga cagtcttgac gtgcgcagct caggggcatg atgtgactgt cgcccgtaca
     9961 tttagcccat acatccccat gtataatcat ttgcatccat acattttgat ggccgcacgg
    10021 cgcgaagcaa aaattacggc tcctcgctcc agacctgcga gcagggaaac gctcccctca
    10081 cagacgcgtt gaattgtccc cacgccgcgc ccctgtagag aaatataaaa ggttaggatt
    10141 tgccactgag gttcttcttt catatacttc cttttaaaat cttgctagga tacagttctc
    10201 acatcacatc cgaacataaa caaaaatggc taaattaaca tctgccgttc ctgttttaac
    10261 agctagggat gttgcaggtg ctgtagagtt ttggacagat aggttaggat tctcaagaga
    10321 ctttgttgag gacgattttg ctggtgttgt cagggatgac gttactttat ttatctcagc
    10381 agtccaagat caagttgtcc ctgataatac attggcttgg gtctgggtca ggggtttaga
    10441 tgaattatat gctgaatggt cagaagttgt atctacaaac ttcagagatg cttctggtcc
    10501 agctatgacc gagattggtg aacagccatg gggtagagaa tttgctttga gagatccagc
    10561 tggaaattgt gttcattttg ttgctgaaga acaagattaa agtaactgac aataaaaaga
    10621 ttcttgtttt caagaacttg tcatttgtat agttttttta tattgtagtt gttctatttt
    10681 aatcaaatgt tagcgtgatt tatatttttt ttcgcctcga catcatctgc ccagatgcga
    10741 agttaagtgc gcagaaagta atatcatgcg tcaatcgtat gtgaatgctg gtcgctatac
    10801 tggagttatc gtgttgcatc tgcggcttta aattgatgta tctcatcgca ggcacgggca
    10861 gtacagtgcc ctgagcgtag ggaaaaatga aaaaaaggat gtaactttta acataattcc
    10921 agcacgcagc gattgggtat aatgaagatt gttaagttca acaacatgtg aaattcttag
    10981 ctccgcaact tgcttgaatt atgagctcta agattcaaga agtaaatagg tcatataatg
    11041 gaatgatagt agtacttgat aaaggaacca tgtgatctta cgttgatatg aaaataattc
    11101 tctcacagaa gtcagtttgt atgactaaca tagagagtaa ttgcttttcg gataaaatcg
    11161 cacctaacaa agattacctt ttgtaaagcc tccagaacag ctatgaatgt tcgttattct
    11221 acgcactttc tttcatacat cttgatccgc cttttttgat gtaggtttta acgcggcaaa
    11281 acatattaat ttttctgatt tggaaaaata aagagttagt tc
//
