LOCUS       sequence-363424-        6450 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Microtubule-targeted Dronpa chromophore-removed FLINC (DrFLINC) 
            pair. Fluctuating red-fluorescent label for live-cell 
            super-resolution imaging..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6450)
  AUTHORS   Lin W, Mo GCH, Mehta S, Zhang J
  TITLE     DrFLINC Contextualizes Super-resolution Activity Imaging.
  JOURNAL   J Am Chem Soc. 2021 Sep 22;143(37):14951-14955. doi: 
            10.1021/jacs.1c05530. Epub 2021 Sep 13.
  PUBMED    34516108
REFERENCE   2  (bases 1 to 6450)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.1c05530"; journalName: "J Am Chem Soc"; date: 
            "2021-09-22- 22"; volume: "143"; issue: "37"; pages: "14951-14955"
COMMENT     Sequence Label: pDronpa-N1-Tau-uDpTT
FEATURES             Location/Qualifiers
     source          1..6450
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        158..461
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        462..665
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     616..636
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      716..725
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1796..2467
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /translation="MSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKVK
                     EGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNAT
                     NDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDVNMALSL
                     EGGGHYRCDFKTTYKAKKVVQLPDYHFVDTHIEIKSHDKDYSNVNLHEHAEAHSELPRQ
                     AK"
     CDS             2492..3187
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHK"
     polyA_signal    3335..3456
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3372..3391)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3426..3445
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3463..3918)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3600..3621)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3812..3831
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3945..4049
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4051..4408
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4051..4242
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4074..4094)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4259..4394
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4443..5237
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4443..5237
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4497..4516)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5107..5126
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5425..5444)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5469..5516
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5845..6433
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6334..6353
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
       61 ccctgattct gtggataacc gtattaccgc catgcattag ttattaatag taatcaatta
      121 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      181 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      241 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      301 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      361 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      421 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      481 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      541 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      601 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      661 gagctggttt agtgaaccgt cagatccgct agcgctaccg gactcagatc tcgaggccac
      721 catggctgag ccccgccagg agttcgaagt gatggaagat cacgctggga cgtacgggtt
      781 gggggacagg aaagatcagg ggggctacac catgcaccaa gaccaagagg gtgacacgga
      841 cgctggcctg aaagctgaag aagcaggcat tggagacacc cccagcctgg aagacgaagc
      901 tgctggtcac gtgacccaag ctcgcatggt cagtaaaagc aaagacggga ctggaagcga
      961 tgacaaaaaa gccaaggggg ctgatggtaa aacgaagatc gccacaccgc ggggagcagc
     1021 ccctccaggc cagaagggcc aggccaacgc caccaggatt ccagcaaaaa ccccgcccgc
     1081 tccaaagaca ccacccagct ctggtgaacc tccaaaatca ggggatcgca gcggctacag
     1141 cagccccggc tccccaggca ctcccggcag ccgctcccgc accccgtccc ttccaacccc
     1201 acccacccgg gagcccaaga aggtggcagt ggtccgtact ccacccaagt cgccgtcttc
     1261 cgccaagagc cgcctgcaga cagcccccgt gcccatgcca gacctgaaga atgtcaagtc
     1321 caagatcggc tccactgaga acctgaagca ccagccggga ggcgggaagg tgcaaatagt
     1381 ctacaaacca gttgacctga gcaaggtgac ctccaagtgt ggctcattag gcaacatcca
     1441 tcataaacca ggaggtggcc aggtggaagt aaaatctgag aagcttgact tcaaggacag
     1501 agtccagtcg aagattgggt ccctggacaa tatcacccac gtccctggcg gaggaaataa
     1561 aaagattgaa acccacaagc tgaccttccg cgagaacgcc aaagccaaga cagaccacgg
     1621 ggcggagatc gtgtacaagt cgccagtggt gtctggggac acgtctccac ggcatctcag
     1681 caatgtctcc tccaccggca gcatcgacat ggtagactcg ccccagctcg ccacgctagc
     1741 tgacgaggtg tctgcctccc tggccaagca gggtttgagc tccggagggg atccaatgag
     1801 tgtgattaaa ccagacatga agatcaagct gcgtatggaa ggcgctgtaa atggacaccc
     1861 gttcgcgatt gaaggagttg gccttgggaa gcctttcgag ggaaaacaga gtatggacct
     1921 taaagtcaaa gaaggcggac ctctgccttt cgcctatgac atcttgacaa ctgtgttctg
     1981 ttacggcaac agggtattcg ccaaataccc agaaaatata gtagactatt tcaagcagtc
     2041 gtttcctgag ggctactctt gggaacgaag catgaattac gaagacgggg gcatttgtaa
     2101 cgcgacaaac gacataaccc tggatggtga ctgttatatc tatgaaattc gatttgatgg
     2161 tgtgaacttt cctgccaatg gtccagttat gcagaagagg actgtgaaat gggagccatc
     2221 cactgagaaa ttgtatgtgc gtgatggagt gctgaagggt gatgttaaca tggctctgtc
     2281 gcttgaagga ggtggccatt accgatgtga cttcaaaact acttataaag ctaagaaggt
     2341 tgtccagttg ccagactatc actttgtgga cacacacatt gagattaaaa gccacgacaa
     2401 agattacagt aatgttaatc tgcatgagca tgccgaagcg cattctgagc tgccgaggca
     2461 ggccaagaga tctgacgagg tggacggtac catgagcgag ctgattaagg agaacatgca
     2521 catgaagctg tacatggagg gcaccgtgaa caaccaccac ttcaagtgca catccgaggg
     2581 cgaaggcaag ccctacgagg gcacccagac catgagaatc aaggtggtcg agggcggccc
     2641 tctccccttc gccttcgaca tcctggctac cagcttcatg tacggcagca gaaccttcat
     2701 caaccacacc cagggcatcc ccgatttctt taagcagtcc ttccctgagg gcttcacatg
     2761 ggagagagtc accacatacg aagacggggg cgtgctgacc gctacccagg acaccagcct
     2821 ccaggacggc tgcctcatct acaacgtcaa gatcagaggg gtgaacttcc catccaacgg
     2881 ccctgtgatg cagaagaaaa cactcggctg ggaggccaac accgagatgc tgtaccccgc
     2941 tgacggcggc ctggaaggca gaaccgacat ggccctgaag ctcgtgggcg ggggccacct
     3001 gatctgcaac ttcaagacca catacagatc caagaaaccc gctaagaacc tcaagatgcc
     3061 cggcgtctac tatgtggacc acagactgga aagaatcaag gaggccgaca aagagaccta
     3121 cgtcgagcag cacgaggtgg ctgtggccag atactgcgac ctccctagca aactggggca
     3181 caagtaagaa ttctgcagat atccatcaca ctggcggccg cgactctaga tcataatcag
     3241 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     3301 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     3361 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     3421 tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata
     3481 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     3541 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     3601 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     3661 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     3721 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     3781 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     3841 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     3901 cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     3961 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     4021 tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt
     4081 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     4141 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     4201 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     4261 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     4321 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     4381 cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc
     4441 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     4501 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     4561 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     4621 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     4681 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     4741 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     4801 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     4861 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     4921 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg
     4981 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     5041 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     5101 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     5161 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     5221 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
     5281 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     5341 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     5401 cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg
     5461 cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa
     5521 acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg
     5581 ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc
     5641 agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat
     5701 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     5761 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     5821 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     5881 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     5941 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     6001 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     6061 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     6121 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     6181 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     6241 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     6301 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     6361 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     6421 gagcctatgg aaaaacgcca gcaacgcggc
//
