LOCUS       sequence-363426-       10178 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lysosome targeted inositol polyphosphate-4-phosphatase type II B; 
            tagged with mCherry..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10178)
  AUTHORS   Chen M, Sun T, Zhong Y, Zhou X, Zhang J
  TITLE     A Highly Sensitive Fluorescent Akt Biosensor Reveals 
            Lysosome-Selective Regulation of Lipid Second Messengers and Kinase 
            Activity.
  JOURNAL   ACS Cent Sci. 2021 Dec 22;7(12):2009-2020. doi: 
            10.1021/acscentsci.1c00919. Epub 2021 Dec 3.
  PUBMED    34963894
REFERENCE   2  (bases 1 to 10178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acscentsci.1c00919"; journalName: "ACS Cent Sci"; date: 
            "2021-12-22- 22"; volume: "7"; issue: "12"; pages: "2009-2020"
COMMENT     Sequence Label: pcDNA3-Lyso-INPP4B-mCherry
FEATURES             Location/Qualifiers
     source          1..10178
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      901..910
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      905..914
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(907..990,991..2157)
                     /codon_start=1
                     /product="human lysosome-associated membrane glycoprotein 
                     1"
                     /label=LAMP1
                     /translation="MAAPGSARRPLLLLLLLLLLGLMHCASAAMFMVKNGNGTACIMAN
                     FSAAFSVNYDTKSGPKNMTFDLPSDATVVLNRSSCGKENTSDPSLVIAFGRGHTLTLNF
                     TRNATRYSVQLMSFAYNLSDTHLFPNASSKEIKTVESITDIRADIDKKYRCVSGTQVHM
                     NNVTVTPHDATIQAYLSNSSFSRGETRCEQDRPSPTTAPPAPPSPSPSPVPKSPSVDKY
                     NVSGTNGTCLLASMGLQLNLTYERKDNTTVTRLLNINPNKTSASGSCGAHLVTLELHSE
                     GTTVLLFQFGMNASSSRFFLQGIQLNTILPDARDPAFKAANGSLRALQATVGNSYKCNA
                     EEHVRVTKAFSVNIFKVWVQAFKVEGGQFGSVEECLLDENSMLIPIAVGGALAGLVLIV
                     LIAYLVGRKRSHAGYQTI"
     CDS             4960..5670
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5105..5123)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5373..5392
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        complement(5731..5749)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5732..5749)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5769..5786)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5775..5999
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6045..6473
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6132..6151)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6342..6363
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6482..6502)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6487..6816
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6667..6802
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6729..6748
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6883..7677
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6937..6956)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7547..7566
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7851..7972
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7888..7907)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7942..7961
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8021..8037)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8021..8037)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8034..8056)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8045..8061
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8069..8075,8076..8093,8094..8099))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8114..8135
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8252..8269)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8423..9011)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8503..8522)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9182..9973,9974..10042))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9805..9824
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10043..10147)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgcggcc
      901 gccaccatgg cggcgcccgg cagcgcccgg cgacccctgc tgctgctact gctgttgctg
      961 ctgctcggcc tcatgcattg tgcgtcagca gcaatgttta tggtgaaaaa tggcaacggg
     1021 accgcgtgca taatggccaa cttctctgct gccttctcag tgaactacga caccaagagt
     1081 ggccctaaga acatgacctt tgacctgcca tcagatgcca cagtggtgct caaccgcagc
     1141 tcctgtggaa aagagaacac ttctgacccc agtctcgtga ttgcttttgg aagaggacat
     1201 acactcactc tcaatttcac gagaaatgca acacgttaca gcgtccagct catgagtttt
     1261 gcttataact tgtcagacac acaccttttc cccaatgcga gctccaaaga aatcaagact
     1321 gtggaatcta taactgacat cagggcagat atagataaaa aatacagatg tgttagtggc
     1381 acccaggtcc acatgaacaa cgtgaccgta acgccccatg atgccaccat ccaggcgtac
     1441 ctttccaaca gcagcttcag caggggagag acacgctgtg aacaagacag gccttcccca
     1501 accacagcgc cccctgcgcc acccagcccc tcgccctcac ccgtgcccaa gagcccctct
     1561 gtggacaagt acaacgtgag cggcaccaac gggacctgcc tgctggccag catggggctg
     1621 cagctgaacc tcacctatga gaggaaggac aacacgacgg tgacaaggct tctcaacatc
     1681 aaccccaaca agacctcggc cagcgggagc tgcggcgccc acctggtgac tctggagctg
     1741 cacagcgagg gcaccaccgt cctgctcttc cagttcggga tgaatgcaag ttctagccgg
     1801 tttttcctac aaggaatcca gttgaataca attcttcctg acgccagaga ccctgccttt
     1861 aaagctgcca acggctccct gcgagcgctg caggccacag tcggcaattc ctacaagtgc
     1921 aacgcggagg agcacgtccg tgtcacgaag gcgttttcag tcaatatatt caaagtgtgg
     1981 gtccaggctt tcaaggtgga aggtggccag tttggctctg tggaggagtg tctgctggac
     2041 gagaacagca tgctgatccc catcgctgtg ggtggtgccc tggcggggct ggtcctcatc
     2101 gtcctcatcg cctacctcgt cggcaggaag aggagtcacg caggctacca gactatcggt
     2161 ggtggcggtg gtgcggatcc catggaaatt aaagaggaag gggcatcaga agaagggcag
     2221 cactttcttc ctacagccca ggccaatgat cccggggact gtcagttcac aagtatccag
     2281 aagactccaa atgaaccgca gttggaattc atccttgcat gcaaggatct cgtggctcct
     2341 gtccgtgatc gtaaactgaa tacactggtg cagatctccg taatccaccc cgtggagcag
     2401 agtctgacaa gatactccag caccgaaatt gtggagggaa caagggaccc actgtttttg
     2461 actggtgtca cattcccatc tgagtatccc atctatgagg agaccaaaat aaaactaaca
     2521 gtctatgatg tcaaggataa gtctcatgac accgttcgaa ccagtgtcct accagaacat
     2581 aaggatcccc cgccagaagt tgggcgaagt ttcttgggct atgccagttt taaagtggga
     2641 gagctgctga agtcaaagga gcaattgctg gtcctgagcc tgagaacttc agatggtggc
     2701 aaagtggttg gcaccataga agtcagtgtc gtgaagatgg gggagattga ggatggggaa
     2761 gccgaccaca tcaccacaga tgtacaggga caaaagtgtg ccctggtatg tgaatgtaca
     2821 gccccggaaa gtgtgagcgg aaaagataac ttaccttttt tgaattcagt gttaaagaac
     2881 ccagtatgta aattatatag atttcccaca tctgacaata agtggatgcg aattcgagag
     2941 cagatgtcag agagcattct ttcctttcat attcctaagg aattgatttc ccttcacatt
     3001 aaagaagatt tgtgcagaaa ccaggagata aaagaacttg gtgagctttc tccacattgg
     3061 gacaatctgc gaaaaaatgt ccttactcac tgtgatcaaa tggtgaatat gtaccaagac
     3121 attctgacag aacttagcaa ggaaacaggg tcctctttca aatcaagcag cagcaaagga
     3181 gagaaaacat tagaatttgt tccaataaat ctacatctgc aaagaatgca ggtacacagc
     3241 cctcacttga aagatgctct ctacgatgtc atcactgtgg gagccccagc tgcccatttt
     3301 cagggattta agaatggtgg tcttcggaag ctactccata gatttgaaac agaaagaaga
     3361 aataccggat accagtttat ttactattca cctgaaaaca cagccaaagc aaaggaagtt
     3421 ctcagcaaca tcaatcaact acaacctctt atagcaaccc atgcagacct actgcttaat
     3481 tctgcaagcc agcattctcc agacagcttg aagaattctt taaagatgct ttcagaaaaa
     3541 acagagcttt ttgtacatgc cttcaaggat caacttgtca ggagtgctct tttagcactc
     3601 tacactgcaa ggccaggagg cattcttaag aagccaccct ctcctaagag cagcacagag
     3661 gagagcagtc cccaagacca acccccagtg atgagagggc aggactccat accacatcat
     3721 tcagactatg atgaggaaga gtgggacagg gtgtgggcca atgtggggaa gagcctgaac
     3781 tgcattattg ctatggtgga caaactgatt gaaagagatg gtggcagtga aggcagtggc
     3841 ggcaacaatg atggagaaaa ggaaccttca ttaacagatg ccattccctc tcacccaaga
     3901 gaggactggt atgaacagtt gtatcccctc atccttaccc tgaaggactg catgggagaa
     3961 gtggtgaacc gagccaagca gtccctgaca tttgtgctcc ttcaggaact tgcgtacagc
     4021 ttgccccagt gtctgatgct gacgctaaga agagacatcg tcttcagcca agcacttgct
     4081 ggattggttt gtggttttat catcaaatta cagacaagtc tgtatgaccc aggcttccta
     4141 cagcagcttc acacagtggg gttgatagta caatatgaag gactgctaag tacatacagc
     4201 gatgaaattg gaatgctaga ggacatggcc gttggcattt ccgatttaaa gaaagttgca
     4261 tttaaaataa ttgaagccaa atccaatgat gtgttgccag ttataacagg aagacgagaa
     4321 cattacgtgg tagaggtcaa gcttccagcc agaatgtttg agtcactacc tctacagatt
     4381 aaagaaggac agttgcttca tgtgtatcca gtacttttta atgttggaat caatgaacag
     4441 caaactctgg ctgaaaggtt tggagatgtc tctttgcaag aaagtattaa tcaggaaaac
     4501 ttcgaacttc tacaagaata ttacaagata tttatggaaa agatgcctcc tgattatatt
     4561 tcacattttc aggaacaaaa tgatttaaaa gcattgctag aaaatctcct tcaaaatatc
     4621 caatccaaaa aaagaaagaa tgtagaaatt atgtggctgg ctgcaacgat ttgccgcaaa
     4681 ctgaatggta ttcgtttcac ctgttgtaaa agtgccaaag acaggacatc gatgtcagtg
     4741 acacttgaac aatgctcaat cttgagagat gagcaccagt tacacaagga cttctttatc
     4801 cgagcgctgg attgcatgag aagagaagga tgccgcatag agaatgtact gaagaatatc
     4861 aaatgcagaa agtatgcttt caacatgcta cagctgatgg ctttccccaa gtactacaga
     4921 cctccagagg ggacttatgg aaaagctgac accgaattca tggtgagcaa gggcgaggag
     4981 gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg
     5041 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     5101 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
     5161 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
     5221 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     5281 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg
     5341 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc
     5401 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag
     5461 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag
     5521 gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc
     5581 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc
     5641 accggcggca tggacgagct gtacaagtaa gaattctgca gatatccatc acactggcgg
     5701 ccgctcgagc atgcatctag agggccctat tctatagtgt cacctaaatg ctagagctcg
     5761 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     5821 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     5881 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     5941 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     6001 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
     6061 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     6121 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     6181 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     6241 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     6301 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     6361 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     6421 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg
     6481 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     6541 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     6601 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     6661 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     6721 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
     6781 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc
     6841 attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga
     6901 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     6961 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     7021 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg
     7081 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     7141 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     7201 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     7261 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     7321 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     7381 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg
     7441 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     7501 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     7561 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     7621 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg
     7681 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg
     7741 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     7801 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
     7861 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     7921 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     7981 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     8041 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     8101 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     8161 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     8221 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     8281 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     8341 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     8401 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     8461 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     8521 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     8581 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     8641 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     8701 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     8761 cgccactggc agcagccact ggtaacagga ttagcaaagc gaggtatgta ggcggtgcta
     8821 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     8881 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     8941 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     9001 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     9061 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     9121 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     9181 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     9241 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     9301 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     9361 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     9421 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     9481 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     9541 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     9601 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     9661 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     9721 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     9781 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     9841 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     9901 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     9961 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
    10021 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
    10081 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
    10141 ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
