LOCUS       sequence-363427-        8684 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Genetically encoded FRET-based cAMP indicator fused to AKAP79..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8684)
  AUTHORS   Tenner B, Getz M, Ross B, Ohadi D, Bohrer CH, Greenwald E, Mehta S, 
            Xiao J, Rangamani P, Zhang J
  TITLE     Spatially compartmentalized phase regulation of a Ca(2+)-cAMP-PKA 
            oscillatory circuit.
  JOURNAL   Elife. 2020 Nov 17;9. pii: 55013. doi: 10.7554/eLife.55013.
  PUBMED    33201801
REFERENCE   2  (bases 1 to 8684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-11-17"; volume: "9. pii: 55013. doi"; pages: " 
            10.7554/eLife.55013"
COMMENT     Sequence Label: pcDNA3-AKAP79-(Ci/Ce)-Epac2-camps
FEATURES             Location/Qualifiers
     source          1..8684
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      895..904
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             907..936
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             949..960
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             join(2254..2256,2257..2259,2260..2970)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3460..3462,3463..4176)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     promoter        complement(4237..4255)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4238..4255)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4275..4292)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4281..4505
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4551..4979
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4638..4657)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4848..4869
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4988..5008)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4993..5322
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5173..5308
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5235..5254
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5389..6183
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5443..5462)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6053..6072
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6357..6478
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6394..6413)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6448..6467
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6527..6543)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6527..6543)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6540..6562)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6551..6567
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6575..6581,6582..6599,6600..6605))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6620..6641
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6758..6775)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6929..7517)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7009..7028)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7688..8479,8480..8548))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8311..8330
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8549..8653)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgccacc
      901 atgggccatc atcatcatca tcatcatcat catcacagca gcggccatat cgaaggtcgt
      961 catatggaaa ccacaatttc agaaattcat gtagaaaaca aggatgagaa gagatcagca
     1021 gaaggtagtc ctggggctga aaggcagaag gaaaaggcat ccatgctttg cttcaagaga
     1081 agaaagaaag cagccaaagc actgaagccc aaagctggct ctgaagctgc tgatgtggca
     1141 aggaagtgtc cacaagaagc aggagcttct gatcagccag agcccacacg gggggcctgg
     1201 gcctcactca aacgtcttgt aacacgcagg aaaaggtcag agtcttcaaa gcagcaaaag
     1261 ccattggagg gtgaaatgca acctgcaata aatgctgagg atgctgatct ttctaagaaa
     1321 aaggcaaaat ctagacttaa gattccctgc ataaaattcc caagagggcc aaaaaggagt
     1381 aatcattcca aaattataga agactcagac tgcagcatca aagtccagga agaagctgaa
     1441 attttggata tacaaacaca gaccccattg aatgatcagg caacaaaggc taagtcaacc
     1501 caggatctaa gtgaaggcat ctcacagaaa gatggtgatg aggtctgtga atcaaatgtg
     1561 agcaatagca taacttctgg agagaaagtg atttcagtag aacttggatt agataatggg
     1621 cattctgcta ttcaaacggg aactctaatc cttgaagaaa ttgaaacgat caaggaaaaa
     1681 caagatgttc aaccccagca agcaagccca cttgaaactt cagaaacaga ccatcagcag
     1741 ccagtacttt ctgatgttcc tcctttacct gcaattccag atcaacaaat tgtggaagaa
     1801 gccagtaaca gtaccctaga aagtgcacca aatggaaaag actatgaaag tacagagatt
     1861 gtagctgaag aaactaagcc aaaagatact gaattgagcc aagaatcaga ttttaaagaa
     1921 aatgggatca ctgaagagaa atccaaatca gaagaaagca aaagaatgga gccaattgct
     1981 attattatta cagacactga aatcagtgaa tttgatgtta caaaatctaa aaatgtccct
     2041 aagcaattct taatttcagc tgaaaatgag caagtagggg tttttgctaa tgataatggt
     2101 tttgaggata gaacttcaga acaatatgaa acactcttaa ttgaaacagc ctcttctcta
     2161 gtcaagaatg ctattcagtt gtcaatagaa cagctggtta atgaaatggc ctctgatgat
     2221 aataaaataa acaatcttct acagaaggat cccatggtga gcaagggcga ggagctgttc
     2281 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     2341 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     2401 accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcctg
     2461 atgtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     2521 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     2581 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     2641 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     2701 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     2761 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     2821 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc
     2881 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     2941 atcactctcg gcatggacga gctgtacaag gagttcgagg agaagaagga gtgtgatgaa
     3001 gaacttcagg acaccatgct gctgctctca cagatgggcc ctgacgccca catgagaatg
     3061 atcctgcgaa aaccacctgg ccagaggact gtggatgacc tagagattat ctacgatgag
     3121 ctccttcata ttaaagcctt atcccatctc tctaccacag tgaaacggga gttagcaggt
     3181 gttctcattt ttgagtctca cgccaaagga ggaactgtgt tgtttaacca gggggaagaa
     3241 ggaacctcct ggtacatcat tctgaaagga tccgtgaatg tagtcattta tggcaagggt
     3301 gtggtctgca ccctgcacga aggagatgac tttggcaagt tagctctagt gaacgatgct
     3361 ccaagagctg cctccattgt tcttcgggaa gataattgtc acttcctaag agtcgacaag
     3421 gaagacttca atcggattct gagggacgtt gagtctagag tgagcaaggg cgaggagctg
     3481 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     3541 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     3601 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctggggc
     3661 gtgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     3721 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     3781 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     3841 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa cgccatcagc
     3901 gacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc
     3961 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     4021 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccaagctg
     4081 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     4141 gggatcactc tcggcatgga cgagctgtac aagtaagaat tctgcagata tccatcacac
     4201 tggcggccgc tcgagcatgc atctagaggg ccctattcta tagtgtcacc taaatgctag
     4261 agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     4321 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     4381 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     4441 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     4501 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
     4561 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     4621 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     4681 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     4741 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     4801 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     4861 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     4921 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
     4981 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     5041 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     5101 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     5161 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     5221 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
     5281 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
     5341 atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
     5401 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     5461 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     5521 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
     5581 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     5641 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     5701 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     5761 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     5821 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     5881 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc
     5941 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     6001 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     6061 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     6121 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     6181 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
     6241 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     6301 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     6361 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     6421 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     6481 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     6541 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     6601 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     6661 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     6721 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     6781 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     6841 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     6901 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     6961 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     7021 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     7081 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     7141 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     7201 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     7261 acttatcgcc actggcagca gccactggta acaggattag caaagcgagg tatgtaggcg
     7321 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     7381 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     7441 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     7501 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     7561 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     7621 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     7681 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     7741 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     7801 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     7861 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     7921 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     7981 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     8041 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     8101 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     8161 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     8221 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     8281 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     8341 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     8401 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     8461 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     8521 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     8581 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     8641 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//
