LOCUS       sequence-363441-       10445 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Control for biosensor in pMM2_9: BM3R1-HAA1-mTurquoise2 under HAA1p 
            and mCherry under HREsYGP1p with flanks for genome integration into 
            HO locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10445)
  AUTHORS   Mormino M, Siewers V, Nygard Y
  TITLE     Development of an Haa1-based biosensor for acetic acid sensing in 
            Saccharomyces cerevisiae.
  JOURNAL   FEMS Yeast Res. 2021 Sep 11;21(6). pii: 6363685. doi: 
            10.1093/femsyr/foab049.
  PUBMED    34477863
REFERENCE   2  (bases 1 to 10445)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS Yeast 
            Res."; date: "2021-09-11"; volume: "21(6). pii: 6363685. doi"; 
            pages: " 10.1093/femsyr/foab049"
COMMENT     Sequence Label: pMM2_12
FEATURES             Location/Qualifiers
     source          1..10445
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(44..859)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(44..859)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     767..786
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     851..870
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      1084..1672
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1573..1592
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             7870..8580
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVDGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(8015..8033)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8283..8302
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             9044..9847
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
ORIGIN
        1 tgccccgagc ggccgcgtgt tacaaccaat taaccaattc tgattagaaa aactcatcga
       61 gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa
      121 gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct
      181 ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt
      241 caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg
      301 gcaaaagctt atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat
      361 caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgaggcgaa
      421 atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga
      481 acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga
      541 atgctgtttt cccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa
      601 aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat
      661 ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg
      721 gcttcccata caatcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt
      781 tatacccata taaatcagca tccatgttgg aatttaatcg cggcctggag caagacgttt
      841 cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta
      901 ttgttcatga tgatatattt ttatcttgtg caatgtaaca tcagagattt tgagacacaa
      961 cgtggctttg ttgaataaat cgaacttttg ctgagttgaa ggatcagtca tgaccaaaat
     1021 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     1081 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     1141 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     1201 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     1261 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     1321 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     1381 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     1441 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     1501 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     1561 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     1621 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     1681 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     1741 tgcgttatcc cctgattctg tggataaccg tgcggccgcc aattctaatt gtatcgagat
     1801 cacttttcgt gatccgctaa tcagcgacgg tcacattagg tttgccaagt cagggtatga
     1861 accatacgat cagttttcgt gaacctggta cgtatattgt ggcgtttgtg tatattttca
     1921 ttctttgaca acaatcaata ccaacctcaa ataggaaaag taataagttt ggcgttacac
     1981 cccaaaagac gccaaacgga tcgaacttac tcaatagcaa ttagcgagac aaaacctacg
     2041 ttaagacctg taaccgattt atcaaagcac tctgcggttc tttcttggga atattacctg
     2101 gacattttgt gccctcaaga aacgaggctc tacgagcctg ttggagcccc tcagacatta
     2161 gccgccacga atcaaacttt ttacgcgatt cggcccaaat cagtttctca cagatcattc
     2221 gtagagtgaa aaagcacatc gattatttga tacccctttg ggttaattac tgttgaggtc
     2281 tttgccgtaa gttccatcgc tgtccctgaa ttcgcatcta gactgaactg gccgataatt
     2341 gcagacgaac gccttgcaat tagcttctat tacacctgat gactgttttt caatctttat
     2401 gtgatctttt tacacccaat gtgataaagt actagtaaca tgaatactac taaacagatg
     2461 atactttaga gttttcattc caacactcaa ttgtaaaaat aagacaatat tatgattttt
     2521 tggctgtata accacatcta tttagtagca gggtgacatg ctactcccac acaatacttc
     2581 gcgcaagtgg tttagtggta aaatccaacg ttgccatcgt tgggcccccg gttcgattcc
     2641 gggcttgcgc aatttttccc cctcgccctg aagggcgtaa cgttaacgct tccggtaatt
     2701 tgcgaataat ttttcttccc atttcccctt tcttttccct ttaacatgtg gagcatagca
     2761 ggctctttac ttttttacgc attcagacag tcgggaggtc aattggtggt gccggttgtg
     2821 ggaaagaggt aattttattt cctttattgg acatgatttc aatagtcaca ttggggcagg
     2881 aaaaagaaaa gggagaaaaa aattcagtaa agaaaaaaaa taagaaaaag agaaaagaaa
     2941 caagagaggg ctacagattt ctaaatatcc ttttgctgca cattttttat ttacctgcta
     3001 attggtctta gtaatacaga gtcgtgcatt tccactttat aagaaaaaaa aaagggataa
     3061 cagcaccagc acttgattga aaactagaaa gttcacgttt tataagtcct ttccgttctc
     3121 tttatcagtt ataataaaag agccaagaca aaacgtggca gggaagcttt tccaaggaaa
     3181 agagggttgt aatattaaac aaaaaccgta atcaagcaaa ataagacccc gtggtttttt
     3241 atcataggaa aagagttctc tgttgtcact ctaccatatt tgacaagaaa aaggaaacaa
     3301 aagtatagaa aaaaaaaacc taaaaaatat atggaatcta ctcctactaa gcaaaaagcc
     3361 atcttctctg cctccttgtt gttgttcgct gagagaggtt tcgacgctac cactatgcca
     3421 atgattgccg aaaacgctaa ggttggtgct ggtactatct acagatactt caagaacaaa
     3481 gaatccttag ttaatgagtt gtttcaacaa cacgtcaatg aatttttgca atgtatcgaa
     3541 tctggtttgg ctaacgaaag agatggttac agggacggtt tccaccacat cttcgaaggt
     3601 atggtcacct tcaccaagaa ccatcctaga gccttaggtt tcatcaagac ccactctcaa
     3661 ggtacttttt tgaccgaaga atccagattg gcttatcaaa agttggtcga atttgtctgt
     3721 actttcttca gagaaggtca aaagcaaggt gtcatcagaa atttgccaga aaacgctttg
     3781 attgctatct tgttcggttc tttcatggaa gtctacgaaa tgattgaaaa cgattatttg
     3841 tctttgactg atgaattatt aaccggtgtt gaggaatcct tgtgggctgc cttgtctcgt
     3901 caatctggtt ctatggtctt gataaatggc ataaagtatg cctgtgagag gtgcataaga
     3961 ggccatagag taacaacatg caatcataca gatcaaccgc ttatgatgat caaacccaaa
     4021 ggtagacctt ccactacatg cgactattgt aaacaacttc gaaaaaacaa gaatgcaaat
     4081 cctgaaggtg tttgcacgtg tggccggcta gagaagaaaa aactggcaca gaaagccaaa
     4141 gaagaagcaa gagctaaagc caaagaaaaa caaagaaaac agtgtacctg cgggactgat
     4201 gaggtttgca aatatcatgc tcaaaagaga catctaagaa agtccccttc aagttctcaa
     4261 aagaaaggaa gatccatttc tcgttctcaa ccaatgtttg aaagggtatt gtcttctact
     4321 tcacttgaca gcaatatgtt atccggccac ggagcactat cagatacctc tagcatactg
     4381 acgagcacat ttttagacag tgagccgggt gttggtaaaa tttcaaaaga ttaccatcat
     4441 gtcccttcat tggcctccat ttcatcctta caatcctcgc aatcgttaga tcaaaatttc
     4501 agtataccac aaagcccgcc gttatcttca atgtcattta attttctcac gggaaatatc
     4561 aatgaaacca accaaaatca cagtaatcat cagcattcaa aatcaggcaa taactggcaa
     4621 gatagttcgg taagcttgcc agcgaaagct gattcacgtc ttaacatgat ggataaaaac
     4681 aactctgtgg gtcttgacct attaggccat tcaaaacgaa tatcgccgat atcaaactct
     4741 cgtgtgggcg aagttagcgt tccgctagaa gaatatattc cttctgacat tgatggggtt
     4801 ggaagagtta ctgataaaag ctctttggtc tacgattggc catttgatga aagtattgag
     4861 agaaatttca gtacaaccgc aaccgctgca actggtgaaa gtaagttcga cattaacgac
     4921 aactgtaata gaattaatag caaaagttat agtaagacta atagtatgaa tggaaacggt
     4981 atgaacaata gcaataataa taatatcaac agtaatggca acgacaagaa caataacaac
     5041 tcttctagac aagaacatca aggaaatgga ctatttgaca tgtttacaga ttcatcgtcg
     5101 atttcaacgc tttcccgtgc aaacttatta ttgcaagaaa aaattggttc gcaagaaaac
     5161 tctgtcaaac aagaaaacta ttcgaaaaat cctcaacttc gtcatcaatt aacttccaga
     5221 agtagatcat ttattcatca tccggcaaac gagtatttga agaatacttt tggaaattca
     5281 catagtaatg acatcggaaa gggagttgaa gtgctatctt tgacaccgag ttttatggat
     5341 attcccgaaa aagaaagaga aacggaaaga tcgccatcat ccaattacat tactgacaga
     5401 cctttcactc gaaaacctag atcttctagc attgacgtaa accataggta tccacctatg
     5461 gcaccaacaa ccgtagcgac atctcccggt gcattgaaca atgccgtagc aagcaatctc
     5521 gacgatcaac tgagtttaac atcactaaac tctcagccat catcgatagc aaatatgatg
     5581 atggaccctt caaacctagc tgagcaaagt tctattcatt cagttcctca gtcaataaac
     5641 tctccgagaa tgcctaaaac tggaagtcgc caagacaaga acattcacac taagaaggaa
     5701 gaaagaaatc cgctaaataa catacacgat ctgtcacaat tggaaaatgt accagacgag
     5761 atgaaccaaa tgttctcccc accattaaaa agtatgaata gaccggatgc cataagggaa
     5821 aattcatcta gtagtaattt cataatccaa ggaaatagca tgatctctac gccttccgga
     5881 aggaatgacc ttccagatac ctctccaatg agtagtattc aaacagcgtc accaccaagt
     5941 caattactga ccgatcaagg atttgcggat ttggataatt tcatgtcttc gttaggttct
     6001 gtttctaaag gtgaagaatt attcactggt gttgtcccaa ttttggttga attagatggt
     6061 gatgttaatg gtcacaaatt ttctgtctcc ggtgaaggtg aaggtgatgc tacttacggt
     6121 aaattgacct taaaatttat ttgtactact ggtaaattgc cagttccatg gccaacctta
     6181 gtcactactt tatcttgggg tgttcaatgt tttgcaagat acccagatca tatgaaacaa
     6241 catgactttt tcaagtctgc catgccagaa ggttatgttc aagaaagaac tatttttttc
     6301 aaagatgacg gtaactacaa gaccagagct gaagtcaagt ttgaaggtga taccttagtt
     6361 aatagaatcg aattaaaagg tattgatttt aaagaagatg gtaacatttt aggtcacaaa
     6421 ttggaataca attatttctc tgacaatgtt tacatcactg ctgacaaaca aaagaatggt
     6481 atcaaagcta acttcaaaat tagacacaac attgaagatg gtggtgttca attagctgac
     6541 cattatcaac aaaatactcc aattggtgat ggtccagtct tgttaccaga caaccattac
     6601 ttatccactc aatctaagtt atccaaagat ccaaacgaaa agagggacca catggtcttg
     6661 ttagaatttg ttactgctgc tggtattacc ttgggtatgg atgaattgta caaaggatcc
     6721 taactcgaga gcttttgatt aagccttcta gtccaaaaaa cacgtttttt tgtcatttat
     6781 ttcattttct tagaatagtt tagtttattc attttatagt cacgaatgtt ttatgattct
     6841 atatagggtt gcaaacaagc atttttcatt ttatgttaaa acaatttcag gtttaccttt
     6901 tattctgctt gtggtgacgc gtgtatccgc ccgctctttt ggtcacccat gtatgctgcc
     6961 aaaccagatg tcaacacagc tacaacggag caataggaaa attcagtagt cgatttccat
     7021 ggcgaggggc tattgggtaa tgtggcggcg agggctaaac attcggtatc attgattcaa
     7081 tgttctcaca atgttcaaat tctcggatcg ggtatcccta attgggatct ctttccagag
     7141 gcgaggggct tgatgtggga ttgaagacac atgagttttt ttctcaccgt cgaaactttt
     7201 gcttacccgt aaaacatcgg ccattcgttg gtttattatg tgactttttt gtgacttttt
     7261 tttttgcgaa gttttctcgt catccgcggg gcgaggggcg ggcgaaaaag tctctcactt
     7321 ttacccaatc ggggcatgtt atgtggacat gttatgtgtc caagagacac atgagcccaa
     7381 aaagggtaca ctgtctaaat agtttaccct ttacgaatcg aaacttctcg ggcgaggggc
     7441 cgaaaattag cgcagaagtt gacgaaagtt tatacttttc ctaatggaga ggctcaggag
     7501 ctgggcgagg ggatattcgt actctattgc atcttcaaac tccgaagaat ctcaggcgag
     7561 ggggaagccg gcttctcgat gctacagttc aacatcgata ctttgaagaa agaaagcgcc
     7621 tattatatct cttttaccct ggcgagggga attaggaggc gaggggaaaa aagtacctca
     7681 ctaaaaaaac catcatctct gaaatataaa aagcttgata gagggtgacg gcgaggggaa
     7741 cttctgctgt gttctcttgg gttattgctc ttattgaata tggcgagggg ttctttcttg
     7801 cttgtaaaaa atcagctcaa aaaacatcta caggattaat cgtcagttaa gtaatacagt
     7861 aatagaaata tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
     7921 cgcttcaagg tgcacatgga gggctccgtg gacggccacg agttcgagat cgagggcgag
     7981 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
     8041 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
     8101 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
     8161 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
     8221 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
     8281 ggccccgtaa tgcagaagaa gactatgggc tgggaggcct cctccgagcg gatgtacccc
     8341 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
     8401 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
     8461 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
     8521 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtaa
     8581 atcctaactc gaggccaatt ggtgcggcaa ttgataataa cgaaaatgtc ttttaatgat
     8641 ctgggtataa tgaggaattt tccgaacgtt tttactttat atatatatat acatgtaaca
     8701 tatattctat acgctataga gaaaggaaat ttttcaatta aaaaaaaaat agagaaagag
     8761 tttcacttct tgattatcgc taacactaat ggttgaagta ctgctacttt aattttatgc
     8821 tgagcagtta cagagatgtt acgaaccact agtgcactgc agtacacggt ttccttgaaa
     8881 tttttttgat tcggtaatct ccgaacagaa ggaagaacga aggaaggagc acagacttag
     8941 attggtatat atacgcatat gtagtgttga agaaacatga aattgcccag tattcttaac
     9001 ccaactgcac agaacaaaaa cgtgcaggaa acgaagataa atcatgtcga aagctacata
     9061 taaggaacgt gctgctactc atcctagtcc tgttgctgcc aagctattta atatcatgca
     9121 cgaaaagcaa acaaacttgt gtgcttcatt ggatgttcgt accaccaagg aattactgga
     9181 gttagttgaa gcattaggtc ccaaaatttg tttactaaaa acacatgtgg atatcttgac
     9241 tgatttttcc atggagggca cagttaagcc gctaaaggca ttatccgcca agtacaattt
     9301 tttactcttc gaggacagaa aatttgctga cattggtaat acagtcaaat tgcagtactc
     9361 tgcgggtgta tacagaatag cagaatgggc agacattacg aatgcacacg gtgtggtggg
     9421 cccaggtatt gttagcggtt tgaagcaggc ggcagaagaa gtaacaaagg aacctagagg
     9481 ccttttgatg ttagcagaat tgtcatgcaa gggctcccta tctactggag aatatactaa
     9541 gggtactgtt gacattgcga agagcgacaa agattttgtt atcggcttta ttgctcaaag
     9601 agacatgggt ggaagagatg aaggttacga ttggttgatt atgacacccg gtgtgggttt
     9661 agatgacaag ggagatgcat tgggtcaaca gtatagaacc gtggatgatg tggtttctac
     9721 aggatctgac attattattg ttggaagagg actatttgca aagggaaggg atgctaaggt
     9781 agagggtgaa cgttacagaa aagcaggctg ggaagcatat ttgagaagat gcggccagca
     9841 aaactaaaaa actgtattat aagtaaatgc atgtatacta aactcacaaa ttagagcttc
     9901 aatttaatta tatcagttat tacccgagtt atcgtgttgc atctgcggct ttaaattgat
     9961 gtatctcatc gcaggcacgg gcagtacagt gccctgagcg tagggaaaaa tgaaaaaaag
    10021 gatgtaactt ttaacataat tccagcacgc agcgattggg tataatgaag attgttaagt
    10081 tcaacaacat gtgaaattct tagctccgca acttgcttga attatgagct ctaagattca
    10141 agaagtaaat aggtcatata atggaatgat agtagtactt gataaaggaa ccatgtgatc
    10201 ttacgttgat atgaaaataa ttctctcaca gaagtcagtt tgtatgacta acatagagag
    10261 taattgcttt tcggataaaa tcgcacctaa caaagattac cttttgtaaa gcctccagaa
    10321 cagctatgaa tgttcgttat tctacgcact ttctttcata catcttgatc cgcctttttt
    10381 gatgtaggtt ttaacgcggc aaaacatatt aatttttctg atttggaaaa ataaagagtt
    10441 agttc
//
