LOCUS       sequence-363442-       10171 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Extracellular domain truncated. Used to test SIRPA exclusion from 
            the phagocytic synpase..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10171)
  AUTHORS   Morrissey MA, Kern N, Vale RD
  TITLE     CD47 Ligation Repositions the Inhibitory Receptor SIRPA to Suppress 
            Integrin Activation and Phagocytosis.
  JOURNAL   Immunity. 2020 Aug 18;53(2):290-302.e6. doi: 
            10.1016/j.immuni.2020.07.008. Epub 2020 Aug 7.
  PUBMED    32768386
REFERENCE   2  (bases 1 to 10171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.immuni.2020.07.008"; journalName: "Immunity"; date: 
            "2020-08-18- 18"; volume: "53"; issue: "2"; pages: "290-302.e6"
COMMENT     Sequence Label: pMM95
FEATURES             Location/Qualifiers
     source          1..10171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    49..166
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        319..726
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             820..1098
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     CDS             join(1255..1257,1258..1260,1261..1974)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1300..1321)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1561..1580)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1908..1929
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2034..2622
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2087..2107)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2505..2516)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2625..2641)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2626..2642)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2732..2965
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(2990..3008)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2991..3008)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3298..3317)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3531..3550)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3650..3672
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3710..3728)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3796..3900
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3901..3969,3970..4761)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4119..4138)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4932..5520
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5421..5440
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5674..5691
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5761..5781)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5766..6095
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5946..6081
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7229..7294
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7424..7444
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7869..8003
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7906..7925)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7960..7979
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8171..8804
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8851..8976
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9473..9706
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9891..9935
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 agtgaacgga tctcgacggt atcgccaaat ggcagtattc atccacaatt ttaaaagaaa
       61 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
      121 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
      181 ggacagcaga gatccagttt ggatcgataa gcttgatatc gaattcctgc agccccgata
      241 aaataaaaga ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg
      301 tttggcaagc tagctgcagt aacgccattt tgcaaggcat ggaaaaatac caaaccaaga
      361 atagagaagt tcagatcaag ggcgggtaca tgaaaatagc taacgttggg ccaaacagga
      421 tatctgcggt gagcagtttc ggccccggcc cggggccaag aacagatggt caccgcagtt
      481 tcggccccgg cccgaggcca agaacagatg gtccccagat atggcccaac cctcagcagt
      541 ttcttaagac ccatcagatg tttccaggct cccccaagga cctgaaatga ccctgcgcct
      601 tatttgaatt aaccaatcag cctgcttctc gcttctgttc gcgcgcttct gcttcccgag
      661 ctctataaaa gagctcacaa cccctcactc ggcgcgccag tcctccgaca gactgagtcg
      721 cccggggggg atctggagct ctcgagaatt ctcacgcgta tgtctcgctc cgtggcctta
      781 gctgtgctcg cgctactctc tctttctggc ctggaggcaa tcctctggca tgagatgtgg
      841 catgaaggcc tggaagaggc atctcgtttg tactttgggg aaaggaacgt gaaaggcatg
      901 tttgaggtgc tggagccctt gcatgctatg atggaacggg gcccccagac tctgaaggaa
      961 acatccttta atcaggccta tggtcgagat ttaatggagg cccaagagtg gtgcaggaag
     1021 tacatgaaat cagggaatgt caaggacctc ctccaagcct gggacctcta ttatcatgtg
     1081 ttccgacgaa tctcaaaggg cggaagcgga ggtagtggtg gaagcccccc agaccacatt
     1141 ccttggatta cagctgtact tccaacagtt attatatgtg tgatggtttt ctgtctaatt
     1201 ctatggaaat ggaagaagaa gaagcggcct cggagcgcag acgccagcgg cggcatggtg
     1261 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     1321 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     1381 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     1441 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     1501 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     1561 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     1621 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     1681 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     1741 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     1801 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     1861 agcacccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     1921 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaagcggcc
     1981 gcgactctag agtcgacctg caggcatgca agcttgatat caagcttatc gataatcaac
     2041 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     2101 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     2161 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     2221 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     2281 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     2341 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     2401 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     2461 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     2521 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     2581 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc
     2641 gagggaatta attcgagctc ggtaccttta agaccaatga cttacaaggc agctgtagat
     2701 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     2761 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     2821 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     2881 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     2941 tagtcagtgt ggaaaatctc tagcagcatc tagaattaat tccgtgtatt ctatagtgtc
     3001 acctaaatcg tatgtgtatg atacataagg ttatgtatta attgtagccg cgttctaacg
     3061 acaatatgta caagcctaat tgtgtagcat ctggcttact gaagcagacc ctatcatctc
     3121 tctcgtaaac tgccgtcaga gtcggtttgg ttggacgaac cttctgagtt tctggtaacg
     3181 ccgtcccgca cccggaaatg gtcagcgaac caatcagcag ggtcatcgct agccagatcc
     3241 tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct
     3301 atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt
     3361 gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct
     3421 tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct
     3481 tcctaatgca ggagtcgcat aagggagagc gtcgaatggt gcactctcag tacaatctgc
     3541 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     3601 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     3661 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     3721 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     3781 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     3841 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     3901 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     3961 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     4021 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     4081 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     4141 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     4201 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     4261 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     4321 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     4381 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     4441 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     4501 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     4561 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     4621 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     4681 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     4741 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     4801 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     4861 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     4921 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     4981 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     5041 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     5101 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     5161 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     5221 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     5281 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     5341 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     5401 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     5461 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     5521 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     5581 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     5641 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     5701 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgt
     5761 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     5821 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     5881 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     5941 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     6001 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     6061 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tggacacaag acaggcttgc
     6121 gagatatgtt tgagaatacc actttatccc gcgtcaggga gaggcagtgc gtaaaaagac
     6181 gcggactcat gtgaaatact ggtttttagt gcgccagatc tctataatct cgcgcaacct
     6241 attttcccct cgaacacttt ttaagccgta gataaacagg ctgggacact tcacatgagc
     6301 gaaaaataca tcgtcacctg ggacatgttg cagatccatg cacgtaaact cgcaagccga
     6361 ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg tctgtaccgg
     6421 gtgcgttact ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt atttccagct
     6481 acgatcacga caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag
     6541 gcttcatcgt tattgatgac ctggtggata ccggtggtac tgcggttgcg attcgtgaaa
     6601 tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg
     6661 atgactatgt tgttgatatc ccgcaagata cctggattga acagccgtgg gatatgggcg
     6721 tcgtattcgt cccgccaatc tccggtcgct aatcttttca acgcctggca ctgccgggcg
     6781 ttgttctttt taacttcagg cgggttacaa tagtttccag taagtattct ggaggctgca
     6841 tccatgacac aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa
     6901 acctcgacgc tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca gtcggccctt
     6961 gatggtaaaa ccatccctca ctggtatcgc atgattaacc gtctgatgtg gatctggcgc
     7021 ggcattgacc cacgcgaaat cctcgacgtc caggcacgta ttgtgatgag cgatgccgaa
     7081 cgtaccgacg atgatttata cgatacggtg attggctacc gtggcggcaa ctggatttat
     7141 gagtgggccc cggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa
     7201 actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt
     7261 taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg
     7321 ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc
     7381 tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa
     7441 ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt
     7501 tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct
     7561 atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata acagttataa
     7621 tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta
     7681 tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt
     7741 gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac
     7801 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     7861 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     7921 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     7981 atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac
     8041 ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct
     8101 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa
     8161 acttagtagt tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg
     8221 atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc
     8281 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta
     8341 gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg
     8401 gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac
     8461 gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag
     8521 ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga
     8581 gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga
     8641 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     8701 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     8761 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     8821 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     8881 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     8941 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     9001 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     9061 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     9121 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     9181 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     9241 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     9301 accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     9361 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     9421 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     9481 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     9541 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     9601 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     9661 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     9721 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     9781 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     9841 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     9901 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     9961 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
    10021 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
    10081 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
    10141 gagagagaga cagagacaga tccattcgat t
//
