LOCUS       sequence-363443-       10987 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains 3 fibcon repeats to observe SIRPA exclusion from the 
            phagocytic synapse.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10987)
  AUTHORS   Morrissey MA, Kern N, Vale RD
  TITLE     CD47 Ligation Repositions the Inhibitory Receptor SIRPA to Suppress 
            Integrin Activation and Phagocytosis.
  JOURNAL   Immunity. 2020 Aug 18;53(2):290-302.e6. doi: 
            10.1016/j.immuni.2020.07.008. Epub 2020 Aug 7.
  PUBMED    32768386
REFERENCE   2  (bases 1 to 10987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.immuni.2020.07.008"; journalName: "Immunity"; date: 
            "2020-08-18- 18"; volume: "53"; issue: "2"; pages: "290-302.e6"
COMMENT     Sequence Label: pMM98
FEATURES             Location/Qualifiers
     source          1..10987
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        155..562
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             656..934
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     CDS             join(1907..1909,1910..1912,1913..2626)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1952..1973)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2213..2232)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2560..2581
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2686..3274
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2739..2759)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3157..3168)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3277..3293)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3278..3294)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3384..3617
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3642..3660)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3643..3660)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3950..3969)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4183..4202)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4302..4324
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4362..4380)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4448..4552
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4553..4621,4622..5413)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4771..4790)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5584..6172
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6073..6092
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6326..6343
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6413..6433)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6418..6747
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6598..6733
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7881..7946
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8076..8096
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8521..8655
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8558..8577)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8612..8631
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8823..9456
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9503..9628
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10125..10358
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10543..10587
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    join(10872..10987,1..2)
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ttcgggttta ttacagggac agcagagatc cagtttggat cgataagctt gatatcgaat
       61 tcctgcagcc ccgataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa
      121 agaccccacc tgtaggtttg gcaagctagc tgcagtaacg ccattttgca aggcatggaa
      181 aaataccaaa ccaagaatag agaagttcag atcaagggcg ggtacatgaa aatagctaac
      241 gttgggccaa acaggatatc tgcggtgagc agtttcggcc ccggcccggg gccaagaaca
      301 gatggtcacc gcagtttcgg ccccggcccg aggccaagaa cagatggtcc ccagatatgg
      361 cccaaccctc agcagtttct taagacccat cagatgtttc caggctcccc caaggacctg
      421 aaatgaccct gcgccttatt tgaattaacc aatcagcctg cttctcgctt ctgttcgcgc
      481 gcttctgctt cccgagctct ataaaagagc tcacaacccc tcactcggcg cgccagtcct
      541 ccgacagact gagtcgcccg ggggggatct ggagctctcg agaattctca cgcgtatgtc
      601 tcgctccgtg gccttagctg tgctcgcgct actctctctt tctggcctgg aggcaatcct
      661 ctggcatgag atgtggcatg aaggcctgga agaggcatct cgtttgtact ttggggaaag
      721 gaacgtgaaa ggcatgtttg aggtgctgga gcccttgcat gctatgatgg aacggggccc
      781 ccagactctg aaggaaacat cctttaatca ggcctatggt cgagatttaa tggaggccca
      841 agagtggtgc aggaagtaca tgaaatcagg gaatgtcaag gacctcctcc aagcctggga
      901 cctctattat catgtgttcc gacgaatctc aaagactagt agtggatccc tggatgcacc
      961 tacagacctg caagtaacca atgttacaga caccagcatt acagtcagtt ggaccccacc
     1021 ttctgccact ataactggat atcggataac ttacactcct tccaatgggc caggggagcc
     1081 aaaagaattg acagtacctc cctcttccac atctgtcact attactggct tgaccccagg
     1141 agttgagtat gttgtatctg tatacgcatt gaaagataat caagaatcac caccccttgt
     1201 tggcactcag acaaccctgg atgctcccac agatttgcaa gtaaccaacg taactgacac
     1261 aagcatcact gttagttgga caccaccaag tgctaccatc accggatatc gaatcacata
     1321 tacaccctct aacgggcctg gcgaaccaaa ggagcttact gttcccccaa gcagtactag
     1381 tgttaccata actggcttga cacccggggt cgaatatgtc gtttcagtat atgcacttaa
     1441 agataatcag gaaagtcccc ccttggtagg aacacagaca actctggacg ctcccactga
     1501 cttgcaagtg accaatgtca cagacacaag tataacagta agctggaccc cacctagcgc
     1561 taccataaca ggctatagga taacatatac acctagcaat ggtccaggtg agcctaaaga
     1621 gctgaccgtg cctcccagct ctacaagtgt gacaatcaca ggacttactc ctggcgtcga
     1681 gtatgtagtt tccgtctatg cactgaagga caatcaagag tcaccccctt tggtcgggac
     1741 tcaaacaacc ggcggaagcg gaggtagtgg tggaagcccc ccagaccaca ttccttggat
     1801 tacagctgta cttccaacag ttattatatg tgtgatggtt ttctgtctaa ttctatggaa
     1861 atggaagaag aagaagcggc ctcggagcgc agacgccagc ggcggcatgg tgagcaaggg
     1921 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     1981 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     2041 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     2101 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     2161 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     2221 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     2281 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     2341 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     2401 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     2461 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     2521 gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     2581 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaagcgg ccgcgactct
     2641 agagtcgacc tgcaggcatg caagcttgat atcaagctta tcgataatca acctctggat
     2701 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     2761 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     2821 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     2881 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     2941 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     3001 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     3061 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     3121 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     3181 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     3241 acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgagggaat
     3301 taattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     3361 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     3421 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     3481 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     3541 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     3601 gtggaaaatc tctagcagca tctagaatta attccgtgta ttctatagtg tcacctaaat
     3661 cgtatgtgta tgatacataa ggttatgtat taattgtagc cgcgttctaa cgacaatatg
     3721 tacaagccta attgtgtagc atctggctta ctgaagcaga ccctatcatc tctctcgtaa
     3781 actgccgtca gagtcggttt ggttggacga accttctgag tttctggtaa cgccgtcccg
     3841 cacccggaaa tggtcagcga accaatcagc agggtcatcg ctagccagat cctctacgcc
     3901 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc
     3961 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc
     4021 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca
     4081 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg
     4141 caggagtcgc ataagggaga gcgtcgaatg gtgcactctc agtacaatct gctctgatgc
     4201 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     4261 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     4321 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     4381 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     4441 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     4501 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     4561 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     4621 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     4681 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     4741 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     4801 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     4861 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     4921 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     4981 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     5041 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     5101 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     5161 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     5221 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     5281 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     5341 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     5401 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     5461 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     5521 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     5581 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     5641 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     5701 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     5761 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     5821 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     5881 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     5941 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     6001 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     6061 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     6121 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     6181 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     6241 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     6301 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     6361 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct gtggaatgtg
     6421 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     6481 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     6541 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     6601 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     6661 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     6721 ttttttggag gcctaggctt ttgcaaaaag cttggacaca agacaggctt gcgagatatg
     6781 tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc
     6841 atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac ctattttccc
     6901 ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga gcgaaaaata
     6961 catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc
     7021 ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctgtacc gggtgcgtta
     7081 ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt gtatttccag ctacgatcac
     7141 gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc
     7201 gttattgatg acctggtgga taccggtggt actgcggttg cgattcgtga aatgtatcca
     7261 aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt tgatgactat
     7321 gttgttgata tcccgcaaga tacctggatt gaacagccgt gggatatggg cgtcgtattc
     7381 gtcccgccaa tctccggtcg ctaatctttt caacgcctgg cactgccggg cgttgttctt
     7441 tttaacttca ggcgggttac aatagtttcc agtaagtatt ctggaggctg catccatgac
     7501 acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg aaacctcgac
     7561 gctagtccgc cgctttaatc acggcgcaca accgcctgtg cagtcggccc ttgatggtaa
     7621 aaccatccct cactggtatc gcatgattaa ccgtctgatg tggatctggc gcggcattga
     7681 cccacgcgaa atcctcgacg tccaggcacg tattgtgatg agcgatgccg aacgtaccga
     7741 cgatgattta tacgatacgg tgattggcta ccgtggcggc aactggattt atgagtgggc
     7801 cccggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta
     7861 cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac
     7921 tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt
     7981 ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca tctagtgatg
     8041 atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga aaggtagaag
     8101 accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg tttagtaata
     8161 gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg ctatacaaga
     8221 aaattatgga aaaatattct gtaaccttta taagtaggca taacagttat aatcataaca
     8281 tactgttttt tcttactcca cacaggcata gagtgtctgc tattaataac tatgctcaaa
     8341 aattgtgtac ctttagcttt ttaatttgta aaggggttaa taaggaatat ttgatgtata
     8401 gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt acttgcttta
     8461 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     8521 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     8581 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     8641 tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc
     8701 ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc
     8761 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta
     8821 gttggaaggg ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca
     8881 cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc
     8941 actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc
     9001 caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
     9061 ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg
     9121 agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc
     9181 cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag
     9241 atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct
     9301 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     9361 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     9421 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     9481 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     9541 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     9601 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     9661 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     9721 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     9781 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     9841 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     9901 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     9961 gcaagcggcc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
    10021 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
    10081 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
    10141 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
    10201 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
    10261 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
    10321 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
    10381 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
    10441 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
    10501 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
    10561 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
    10621 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
    10681 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
    10741 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
    10801 gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcca aatggcagta
    10861 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
    10921 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
    10981 caaaatt
//
