LOCUS       sequence-363543-        5476 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of the Salmo salar gamma interferon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5476)
  AUTHORS   Collins C, Ganne G, Collet B
  TITLE     Isolation and activity of the promoters for STAT1 and 2 in Atlantic 
            salmon Salmo salar.
  JOURNAL   Fish Shellfish Immunol. 2014 Oct;40(2):644-7. doi: 
            10.1016/j.fsi.2014.07.025. Epub 2014 Aug 13.
  PUBMED    25128593
REFERENCE   2  (bases 1 to 5476)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.fsi.2014.07.025"; journalName: "Fish Shellfish Immunol"; 
            date: "2014-10"; volume: "40"; issue: "2"; pages: "644-7"
COMMENT     Sequence Label: pcDNA3.1-Zeo-ssIFNG
FEATURES             Location/Qualifiers
     source          1..5476
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(6..26)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11..340
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      191..326
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     253..272
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        388..435
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             454..828
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    958..1079
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(995..1014)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1049..1068
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1128..1144)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1128..1144)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1141..1163)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1152..1168
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1176..1182,1183..1200,1201..1206))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1221..1242
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1359..1376)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1530..2118)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1610..1629)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2289..3080,3081..3149))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2912..2931
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3150..3254)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3329..3348)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3520..3899
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3900..4103
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4054..4074
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4148..4167
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4148..4166
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4769..4786)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4775..4999
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5045..5473
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5132..5151)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5342..5363
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
       61 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
      121 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
      181 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
      241 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
      301 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
      361 atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc
      421 atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg
      481 gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc
      541 tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc
      601 atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc
      661 ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc
      721 tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc
      781 gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga
      841 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
      901 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
      961 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     1021 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     1081 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     1141 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     1201 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     1261 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     1321 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     1381 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     1441 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     1501 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     1561 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     1621 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     1681 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     1741 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     1801 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     1861 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     1921 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     1981 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     2041 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     2101 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     2161 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     2221 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     2281 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     2341 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     2401 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     2461 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     2521 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     2581 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     2641 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     2701 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     2761 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     2821 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     2881 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     2941 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     3001 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     3061 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     3121 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     3181 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     3241 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
     3301 ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     3361 agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag
     3421 ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt
     3481 ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt
     3541 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     3601 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     3661 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     3721 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     3781 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     3841 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     3901 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
     3961 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     4021 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
     4081 gggaggtcta tataagcaga gctctctggc taactagaga acccactgct tactggctta
     4141 tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgttta aacgggccct
     4201 ctagactcga gatggatgtg ttatcaaggg ctgtgatgtg tttctgcttg atgggctgga
     4261 tgactttagg atggagtaat gccgctcagt acacatcaat taacatgaag agcaacatag
     4321 acaaactgaa ggtccactat aagatctcca aggaccagct gttcaacgga aaacctgttt
     4381 tccccaagga cacgtttgag gacagtgagc ggagggtgtg gatgagtgtg gttctggacg
     4441 tgtatcggag tatcttcaac cagatgctga accagacggg ggaccaggaa gtgagggaga
     4501 ggctggacca ggtcaagggg aaggttcagg agacccagaa acactacttc ctgaaaagga
     4561 tacctgagct gaggacacac ctgcagaacc tgtgggccat cgagaccagt aacaccacag
     4621 tccaggggaa ggctctgtcc gagttcatta ccatctacga gaaagcctcc gaactggccc
     4681 ataagaagga caaccgcagg aatagacgac aagcccagag gctcaaatca agcatcatgt
     4741 agaagcttaa gtttaaaccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     4801 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     4861 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     4921 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     4981 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
     5041 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     5101 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     5161 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     5221 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     5281 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     5341 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     5401 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     5461 tttaacgcga attaat
//
