LOCUS       sequence-36355-e         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  TITLE     2-series LIC N-terminal fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET His6 MBP N10 TEV LIC cloning vector (2C-T)
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(101..121)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(179..202)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatcagcgc gcacgcgtgg taccggccgg ccaccaggtg
       61 gcgcgccgcg atcgcggatc cgttatccac ttccaatatt ggattggaag tacaggtttt
      121 cctcgatccc gaggttgttg ttattgttat tgttgttgtt gttcgagctc gaattagtct
      181 gcgcgtcttt cagggcttca tcgacagtct gacgaccgct ggcggcgttg atcaccgcag
      241 tacgcacggc ataccagaaa gcggacatct gcgggatgtt cggcatgatt tcacctttct
      301 gggcgttttc catagtggcg gcaatacgtg gatctttcgc caactcttcc tcgtaagact
      361 tcagcgctac ggcacccagc ggtttgtctt tattaaccgc ttccagacct tcatcagtca
      421 gcagatagtt ttcgaggaac tcttttgcca gctctttgtt cggactggcg gcgttaatac
      481 ctgcgctcag cacgccaacg aacggtttgg atggttgacc cttgaaggtc ggcagtaccg
      541 ttacaccata attcactttg ctggtgtcga tgttggacca tgcccacggg ccgttgatgg
      601 tcatcgctgt ttcgccttta ttaaaggcag cttctgcgat ggagtaatcg gtgtctgcat
      661 tcatgtgttt gtttttaatc aggtcaacca ggaaggtcag acccgctttc gcgccagcgt
      721 tatccacgcc cacgtcttta atgtcgtact tgccgttttc atacttgaac gcataacccc
      781 cgtcagcagc aatcagcggc c
//
