LOCUS       sequence-363554-        6819 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Zebrafish ctnna1 bearing a point mutation in the vinculin binding 
            site. For in vitro transcription (SP6) or expression through CMV.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6819)
  AUTHORS   Boutillon A, Escot S, Elouin A, Jahn D, Gonzalez-Tirado S, Starruss 
            J, Brusch L, David NB
  TITLE     Guidance by followers ensures long-range coordination of cell 
            migration through alpha-catenin mechanoperception.
  JOURNAL   Dev Cell. 2022 May 17. pii: S1534-5807(22)00329-X. doi: 
            10.1016/j.devcel.2022.05.001.
  PUBMED    35613615
REFERENCE   2  (bases 1 to 6819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2022 May 17. pii: S1534-5807(22)00329-X. doi: 
            10.1016/j.devcel.2022.05.001."
COMMENT     Sequence Label: pCS2+ Zf ctnna1L344P
FEATURES             Location/Qualifiers
     source          1..6819
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        25..43
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     25..42
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     polyA_signal    2861..2995
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2885..2904)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2939..2958
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3108..3128)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3108..3126)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3147..3163)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3147..3163)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3160..3182)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3171..3187
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3195..3201,3202..3219,3220..3225))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3240..3261
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3378..3395)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3549..4137)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3629..3648)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4308..5099,5100..5168))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4931..4950
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5169..5273)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5299..5754)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5436..5457)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5648..5667
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5825..6809
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag ctacttgttc
       61 tttttgcagg atcccatcga ttcgaattca agccatgacg agcattaaca ctgctaacat
      121 caacttcaaa tgggatccca agagtttgga gatccgtacg cttgctgtgg aaagactgct
      181 ggagccattg gtaacccagg tcaccacact tgtaaactcc agcaacaaag gtccatctaa
      241 caagaagaag gggcggtcta agaaggctca cgtattggca gcctcagtgg atgaagccac
      301 ccagaacttc ttggagaaag gcgagaagat tgctaaagag agccagttct tgaaggaaga
      361 gctgaccgca gcggtggagg atgttcgcaa gcaaggagaa tccatgaaga tggcctcggg
      421 agagtttgca gaggatcctt gttcttcagt gaaaagaggc aacatggtcc gtgctgcacg
      481 tgcccttctt tctgcagtca ctcatctgct tgttctggct gacatggctg atgtttacca
      541 acttcttctt caactcaaac tggtggagga gaacctgatg aaggtgcgta atgctggcac
      601 tgagcaagac ctgggcatcc aatacaaagc tctgaagcca gaggttgaca aactgaacat
      661 gatggcggcc aagaggcagc aggaactcaa ggacgtccat cacaaagacc aaatggcagc
      721 agctcgcggc gtccttcaga ggaacgttcc catgctgtac acggcttcgc gtgcctgcct
      781 acagcaccca gacgtggccg cgtacaaggc caaccgggat ctgatctaca agcagctgca
      841 gcacgccgtc tctggcatat ctaatgccgc tcaggctacc agcagcgagg attccagctt
      901 ctcccagact gcaggtggag gagagctagc ctacgccctc aacaactttg ataagcagat
      961 catcgtggac ccactgagtt tcagcgagga gagatttcgg ccctctcttg aggaacgtct
     1021 ggaaagcatc attagtggcg ccgccctcat ggccgattcc tcatgcacac gcgacgaccg
     1081 gcgcgaacgc atcgtggctg aatgcaactc tgtgcgccag gcgccgcagg atctgctctc
     1141 tgagtacatg ggcaatgctg gacgtaagga gaagagcgat gccctcaaca cggccattga
     1201 cagaatgact aagaagaccc gtgacctccg cagacagctg cggaaggctg tgatggacca
     1261 cgtgtcggac tcttttctgg agaccaatgt tcctctgctg gtattgatcg aagctgccaa
     1321 gaatggtaat gaaaaggagg tgaaggaata tgcccaagtt ttccgtgagc acgccaacaa
     1381 actgattgag gttgccaacc ttgcctgctc catctccaac aacgaagagg gagtaaagtt
     1441 ggtgcgaatg gccgcttccc agctggaaac actgtgccct caggtgatta atgcagcttt
     1501 ggccctggct gccaaaccta acagcaaggt ggctcaggat aacatggatt tgtttaaaga
     1561 ccagtgggaa aaacaggtgc gagtgctcac tgacgctgtg gatgacatca cttccattga
     1621 cgacttcctc tgcgtgtctg aaaatcacat tctggaggat gtgaacaagt gtgtcatcgc
     1681 tctgcaagaa aaggatgtgg atgggctcga tcgcactgct ggagccattc ggggcagagc
     1741 tgctcgtgtg gtccatgtgg tcacatctga gatggacaac tatatgcctg gcgtatacac
     1801 ggagaaggtt ctggaagcta ccaaacttct tactgaaaca gtgatgccac gtttcaccga
     1861 gcaagtagag gcagcagtgg aggctctcag tgccaacaac gggcagccag tggacgagaa
     1921 cgagttcatt gatgcctcgc gtctggtgta tgatggtgtg agagacatca ggaaggcggt
     1981 gctgatgatc aggaccccag aggaactgga tgactcggac tttgagactg aagactttga
     2041 ttctcgcagc aggaccagcg ttcagacaga agatgatcag ctcattgctg gacagagcgc
     2101 tcgggccatc atggctcagc taccccagga gcagaaggcg aagattgcgg agcaggtggc
     2161 cagtttccag gaggagaaga gcaagctgga tgcagaggtg tccaagtggg atgacagtgg
     2221 aaatgacatc atcgtcttgg ccaagcagat gtgcatgatc atgatggaga tgacagactt
     2281 caccaggggg aaaggaccac tcaaaaacac ctcagatgtg attagtgctg ccaagaagat
     2341 tgctgaagcc ggatcaagaa tggacaagct gggccgcgcc attgcagatc aatgtccaga
     2401 ttcagcgtgc aagcaggatc tgttggcgta tctgcagcgc atcgccctgt tctgtcacca
     2461 gctcaacatc tgcagtaaag tcaaagcgga ggtgcagaat ctgggtggag agctcgtggt
     2521 gtctggactg gacagtgcca tgtctctgat ccaggcggcc aagaatctga tgaattctgt
     2581 ggtgtccaca gtaaaggcct cctacgtggc ctccaccaaa taccagaaat ctcaagatat
     2641 gcaaagcctc aacatgcccg ccatctcctg gaagatgaaa gctcccgaga agaagccgct
     2701 ggtcaaacgc gagaagctgg acgacggtca gaccaataaa gtcaagcggt cctctcaaaa
     2761 gaagcacatc aaccctgtgc aggccctgag cgagttcaaa gctatggata gtatttaagg
     2821 cctctcgagc ctctagaact atagtgagtc gtattacgta gatccagaca tgataagata
     2881 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     2941 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     3001 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaatt
     3061 cgcggccgcg gcgccaatgc attgggcccg gtacccagct tttgttccct ttagtgaggg
     3121 ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     3181 ctcacaattc cacacaacat acgagccggg agcataaagt gtaaagcctg gggtgcctaa
     3241 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     3301 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     3361 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     3421 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     3481 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     3541 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     3601 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     3661 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     3721 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     3781 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     3841 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     3901 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     3961 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     4021 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     4081 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     4141 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     4201 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     4261 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     4321 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     4381 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     4441 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     4501 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     4561 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     4621 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     4681 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     4741 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     4801 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     4861 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     4921 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     4981 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     5041 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     5101 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     5161 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     5221 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     5281 ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
     5341 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     5401 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     5461 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     5521 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     5581 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     5641 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     5701 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt
     5761 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
     5821 gccagtcgac cgccaattca atatggcgta tatggactca tgccaattca atatggtgga
     5881 tctggacctg tgccaattca atatggcgta tatggactcg tgccaattca atatggtgga
     5941 tctggacccc agccaattca atatggcgga cttggcacca tgccaattca atatggcgga
     6001 cctggcactg tgccaactgg ggaggggtct acttggcacg gtgccaagtt tgaggagggg
     6061 tcttggccct gtgccaagtc cgccatattg aattggcatg gtgccaataa tggcggccat
     6121 attggctata tgccaggatc aatatatagg caatatccaa tatggcccta tgccaatatg
     6181 gctattggcc aggttcaata ctatgtattg gccctatgcc atatagtatt ccatatatgg
     6241 gttttcctat tgacgtagat agcccctccc aatgggcggt cccatatacc atatatgggg
     6301 cttcctaata ccgcccatag ccactccccc attgacgtca atggtctcta tatatggtct
     6361 ttcctattga cgtcatatgg gcggtcctat tgacgtatat ggcgcctccc ccattgacgt
     6421 caattacggt aaatggcccg cctggctcaa tgcccattga cgtcaatagg accacccacc
     6481 attgacgtca atgggatggc tcattgccca ttcatatccg ttctcacgcc ccctattgac
     6541 gtcaatgacg gtaaatggcc cacttggcag tacatcaata tctattaata gtaacttggc
     6601 aagtacatta ctattggaag tacgccaggg tacattggca gtactcccat tgacgtcaat
     6661 ggcggtaaat ggcccgcgat ggctgccaag tacatcccca ttgacgtcaa tggggagggg
     6721 caatgacgca aatgggcgtt ccattgacgt aaatgggcgg taggcgtgcc taatgggagg
     6781 tctatataag caatgctcgt ttagggaacc gccattctg
//
