LOCUS       sequence-363556-        4887 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutively active form of acvr1ba (Nodal receptor). For in vitro
            transcription (SP6).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   Boutillon A, Escot S, Elouin A, Jahn D, Gonzalez-Tirado S, Starruss 
            J, Brusch L, David NB
  TITLE     Guidance by followers ensures long-range coordination of cell 
            migration through alpha-catenin mechanoperception.
  JOURNAL   Dev Cell. 2022 May 17. pii: S1534-5807(22)00329-X. doi: 
            10.1016/j.devcel.2022.05.001.
  PUBMED    35613615
REFERENCE   2  (bases 1 to 4887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2022 May 17. pii: S1534-5807(22)00329-X. doi: 
            10.1016/j.devcel.2022.05.001."
COMMENT     Sequence Label: pSP64T acvr1ba*
FEATURES             Location/Qualifiers
     source          1..4887
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     5'UTR           8..50
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     primer_bind     complement(1951..1967)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1951..1967)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1964..1986)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1975..1991
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1999..2005,2006..2023,2024..2029))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2044..2065
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2182..2199)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2353..2941)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2433..2452)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3112..3903,3904..3972))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3735..3754
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3973..4077)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4145..4163
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4201..4223)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4323..4342
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4556..4575
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        4865..4883
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     4865..4882
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 aagcttgctt gttctttttg cagaagctca gaataaacgc tcaactttgg cagatccccc
       61 gggctgcatg ctcgagcggc cgccagtgtg atggatatcg aattccggca gacggtgtat
      121 ggttggtcgg tttgtggttg tgttggaagt ctcgctcgct tgtcaatatc acacacaacg
      181 aatgcattaa tgctaagaga tgggaatgtt gcagtcatgc ctcctcgacg aactgctgta
      241 gccttgctcg ccctgtgtgg attactagcg gtcggcgatg ctctcaagtg taactgcact
      301 gcttgtgaga gcacaggtta tgtgtgcgag acagatgggg cctgcatggc ctccacatcc
      361 tacatcaatg ggcaggaaga gcagcaagta cggatctgca tccctcgggt cagcctggtt
      421 cctcctggac agcccatcta ctgcttgagc gctaaaggcc tgctcaacac gcactgctgt
      481 tacacagact tctgcaacag catcaacctc cagattccca atgggattgc tgatggtaaa
      541 ggaggtagtt ggggtccggt agagctggtg gcagtgatag caggcccggt cttcctcttt
      601 tgcctgctgc ttatcgtggg agtgctcctt ttccagcacc accaacgaaa ctataaccac
      661 cggcagcgac tggatgttga ggatccatcc tgtgatcacc tgtacttggc caaagacaag
      721 accttacagg atctcatctt cgatctgtcc acctccggtt cagggtctgg gctgcccttg
      781 ttcgttcaga ggactgtggc cagggacatt gtactgcagg agatcatagg aaaaggtcgc
      841 tttggggaag tgtggagggg gagatggaga ggaggtgatg tggctgtgaa gatcttctca
      901 tccagggagg aacgttcctg gttccgtgaa gctgagattt accaaaccat catgctccgc
      961 catgaaaaca tcttgggctt cattgctgct gataataaag acaatggcac atggacacag
     1021 ctgtggctag tgtcagacta ccatgagcat ggctcattat ttgactatct caaccactac
     1081 tccgtcacaa tcgaggggat gatcaagtta tcgctttcag ccgccagcgg tctggcacat
     1141 ctgcacatgg agatcctggg cacacagggg aaaccaggca tcgcacatcg tgacctcaaa
     1201 tccaaaaaca tcttggtgaa aaagaatggt acttgcgcca tagcagatct agggctcgct
     1261 gtacggcacg agtccatcac tgatactata gacattgcac ccaatcagag ggtgggcact
     1321 aaaagatata tggccccaga ggtactagat gaaacgatca acatgaagca ttttgattct
     1381 ttcaagtgtg ctgatatcta tgctttggga ctggtatatt gggagattgc acgacggtgt
     1441 aatgctggag gtatccatga agattatcag ctaccctact atgacctggt gccgtccgac
     1501 ccctccatag aagagatgag gaaggtggtg tgtgaccaga ggcttcggcc taatgtgccc
     1561 aactggtggc agagctatga ggcgctgaga gtgatgggaa agatcatgcg ggagtgttgg
     1621 tatgccaacg gagcggcgcg gcttacagct ctgcggatta agaagactct ttcgcaactc
     1681 agcgtccaag aagacattaa gatctgaaac atgcatggta ccaagcttga tgcaggtcga
     1741 cctcgaggat ctggttacca ctaaaccagc ctcaagaaya cmmraatkkr kycycyaarm
     1801 tryakmmwwy yrwmtywbmy yywaaaaaaa aaaaarwyyy wwmmmmwwmw aaaaaaaaaa
     1861 aaaaaaaaaa aaaaaaaamm mmmccccccc cccccccnnn ntgcaggtcg actctagagg
     1921 atccccgggc gagctcgaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta
     1981 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     2041 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     2101 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     2161 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     2221 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     2281 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     2341 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     2401 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2461 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2521 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     2581 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2641 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2701 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2761 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     2821 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     2881 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     2941 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     3001 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     3061 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     3121 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     3181 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     3241 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     3301 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     3361 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     3421 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     3481 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     3541 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     3601 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     3661 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     3721 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     3781 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     3841 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     3901 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     3961 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     4021 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     4081 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     4141 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
     4201 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     4261 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
     4321 tgcggcatca gagcagattg tactgagagt gcaccattcg acgctctccc ttatgcgact
     4381 cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga
     4441 atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac
     4501 ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga
     4561 tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc
     4621 gtccggcgta gaggatctgg ctagcgatga ccctgctgat tggttcgctg accatttccg
     4681 ggtgcgggac ggcgttacca gaaactcaga aggttcgtcc aaccaaaccg actctgacgg
     4741 cagtttacga gagagatgat agggtctgct tcagtaagcc agatgctaca caattaggct
     4801 tgtacatatt gtcgttagaa cgcggctaca attaatacat aaccttatgt atcatacaca
     4861 tacgatttag gtgacactat agaatac
//
