LOCUS       sequence-363565-        7914 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the myc-tagged SHP-1 C453S mutant (SHP-1C/S) 
            (substrate-trapping mutant) and mouse CD8..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7914)
  AUTHORS   Alborzian Deh Sheikh A, Akatsu C, Abdu-Allah HHM, Suganuma Y, 
            Imamura A, Ando H, Takematsu H, Ishida H, Tsubata T
  TITLE     The Protein Tyrosine Phosphatase SHP-1 (PTPN6) but Not CD45 (PTPRC) 
            Is Essential for the Ligand-Mediated Regulation of CD22 in 
            BCR-Ligated B Cells.
  JOURNAL   J Immunol. 2021 Jun 1;206(11):2544-2551. doi: 
            10.4049/jimmunol.2100109. Epub 2021 May 14.
  PUBMED    33990399
REFERENCE   2  (bases 1 to 7914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.4049/jimmunol.2100109"; journalName: "J Immunol"; date: 
            "2021-06-1- 1"; volume: "206"; issue: "11"; pages: "2544-2551"
COMMENT     Sequence Label: pMX-SHP1C/S-ires-mCD8
FEATURES             Location/Qualifiers
     source          1..7914
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             33..626
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    689..1046
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1111..1527
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1457..1479
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1495..1511
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1537..1911
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     CDS             3758..3787
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      4515..4524
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4521..4523,4524..4526,4527..5240)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4521..4523,4524..4526,4527..5237)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4566..4587)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4827..4846)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5174..5195
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5430..5899
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(6029..6046)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6200..6788)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6280..6299)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6952..7743,7744..7812))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7575..7594
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(7813..7914,1..3))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gcgcacattt ccccgaaaag tgccacctgc ataatgaaag accccacctg taggtttggc
       61 aagctagctt aagtaacgcc attttgcaag gcatggaaaa atacataact gagaatagaa
      121 aagttcagat caaggtcagg aacagatgga acagctgaat atgggccaaa caggatatct
      181 gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggaacagc tgaatatggg
      241 ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt
      301 ccccagatgc ggtccagccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc
      361 cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc
      421 ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg
      481 cgcgccagtc ctccgattga ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt
      541 gcagttgcat ccgacttgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga
      601 ctacccgtca gcgggggtct ttcatttggg ggctcgtccg ggatcgggag acccctgccc
      661 agggaccacc gacccaccac cgggaggtaa gctggccagc aacttatctg tgtctgtccg
      721 attgtctagt gtctatgact gattttatgc gcctgcgtcg gtactagtta gctaactagc
      781 tctgtatctg gcggacccgt ggtggaactg acgagttcgg aacacccggc cgcaaccctg
      841 ggagacgtcc cagggacttc gggggccgtt tttgtggccc gacctgagtc caaaaatccc
      901 gatcgttttg gactctttgg tgcacccccc ttagaggagg gatatgtggt tctggtagga
      961 gacgagaacc taaaacagtt cccgcctccg tctgaatttt tgctttcggt ttgggaccga
     1021 agccgcgccg cgcgtcttgt ctgctgcagc atcgttctgt gttgtctctg tctgactgtg
     1081 tttctgtatt tgtctgaaaa tatgggcccg ggccagactg ttaccactcc cttaagtttg
     1141 accttaggtc actggaaaga tgtcgagcgg atcgctcaca accagtcggt agatgtcaag
     1201 aagagacgtt gggttacctt ctgctctgca gaatggccaa cctttaacgt cggatggccg
     1261 cgagacggca cctttaaccg agacctcatc acccaggtta agatcaaggt cttttcacct
     1321 ggcccgcatg gacacccaga ccaggtcccc tacatcgtga cctgggaagc cttggctttt
     1381 gacccccctc cctgggtcaa gccctttgta caccctaagc ctccgcctcc tcttcctcca
     1441 tccgccccgt ctctccccct tgaacctcct cgttcgaccc cgcctcgatc ctccctttat
     1501 ccagccctca ctccttctct aggcgccccc atatggccat atgagatctt atatggggca
     1561 cccccgcccc ttgtaaactt ccctgaccct gacatgacaa gagttactaa cagcccctct
     1621 ctccaagctc acttacaggc tctctactta gtccagcacg aagtctggag acctctggcg
     1681 gcagcctacc aagaacaact ggaccgaccg gtggtacctc acccttaccg agtcggcgac
     1741 acagtgtggg tccgccgaca ccagactaag aacctagaac ctcgctggaa aggaccttac
     1801 acagtcctgc tgaccacccc caccgccctc aaagtagacg gcatcgcagc ttggatacac
     1861 gccgcccacg tgaaggctgc cgaccccggg ggtggaccat cctctagact gccggatccc
     1921 agtgtggtgg tacgggaatt cgaaccccag gatggtgagg tggtttcacc gggacctcag
     1981 cgggcctgat gcagagaccc tgctgaaggg ccggggagtc cctgggagct tcctggctcg
     2041 gcccagccgc aagaaccagg gtgacttctc cctctcagtc agggtggatg atcaggtgac
     2101 tcatattcgg atccagaact caggggactt ctatgacctg tacggagggg agaagtttgc
     2161 gacgctgaca gagctggtcg agtattacac gcagcagcag ggcatcctgc aggaccgaga
     2221 tggcaccatc atccacctta agtacccact gaactgctcg gaccccacca gtgagaggtg
     2281 gtaccacggc cacatatctg gagggcaggc ggagtcactg ctgcaggcca agggcgagcc
     2341 ctggacattt cttgtgcgtg agagtctcag ccaacctggt gattttgtgc tctctgtgct
     2401 caatgaccag cccaaggctg gcccaggttc cccgctcagg gtcactcata tcaaggttat
     2461 gtgtgagggt ggacgctata ctgtgggtgg ctcagagacg tttgacagcc tcacagacct
     2521 ggtggagcac ttcaagaaga cagggattga ggaggcctcg ggtgcctttg tctacctgcg
     2581 gcagccttac tacgctactc gggtaaacgc agctgacatt gagaatcggg tcttggaact
     2641 gaacaagaag caggagtcgg aggacacagc caaggctggc ttctgggagg agtttgagag
     2701 tctacaaaag caggaggtaa agaatctaca ccaacgtctg gaagggcagc ggccagagaa
     2761 caagagcaag aaccgctaca agaacattct tccctttgac cacagccgag tgatcctgca
     2821 gggacgtgac agtaacatcc caggctctga ctacatcaat gccaactacg tgaagaacca
     2881 gctgctaggt ccagatgaga actctaagac ctacatcgcc agccagggct gtctggatgc
     2941 cacagtcaat gacttctggc agatggcttg gcaggagaac actcgtgtca tcgtcatgac
     3001 taccagagag gtggagaaag gccggaacaa atgtgtccca tactggcccg aggtgggcac
     3061 tcagcgtgtc tatggtctct actctgtgac caacagtagg gagcatgaca cagcagaata
     3121 caaactgcga acattacaga tctccccact agacaatggg gacctggttc gggagatatg
     3181 gcactaccag tacctgagct ggcctgacca tggggttccc agtgagcctg ggggtgtcct
     3241 cagctttctg gatcagatca accagcgaca ggaaagtttg cctcatgcag ggcccatcat
     3301 tgtgcactcc agcgctggca tcggccgcac gggcaccatc atcgtcattg atatgcttat
     3361 ggaaagcatc tccaccaagg ggctagactg tgacattgat atccagaaga ccatccagat
     3421 ggtacgagca cagcgctccg gcatggtgca gaccgaggcc cagtacaagt ttatttacgt
     3481 ggccattgcc cagttcatcg aaacgaccaa gaagaaactg gagatcatac aatcccagaa
     3541 gggccaggag tcggagtatg ggaatatcac gtaccctccc gctgtgagga gtgcccacgc
     3601 caaagcctcg cgtacttcct ccaagcacaa ggaggaggtg tacgaaaacg tgcatagcaa
     3661 gagcaagaag gaagagaaag taaagaagca gcggtcggca gacaaggaga agaacaaagg
     3721 ttctctcaag aggaagaagc ttgtcaccgt ctcctcagaa caaaaactca tctcagaaga
     3781 ggatctgaat taagtcgacc tcgagcagct gaagcttaaa acagctctgg ggttgtaccc
     3841 accccagagg cccacgtggc ggctagtact ccggtattgc ggtacccttg tacgcctgtt
     3901 ttatactccc ttcccgtaac ttagacgcac aaaaccaagt tcaatagaag ggggtacaaa
     3961 ccagtaccac cacgaacaag cacttctgtt tccccggtga tgtcgtatag actgcttgcg
     4021 tggttgaaag cgacggatcc gttatccgct tatgtacttc gagaagccca gtaccacctc
     4081 ggaatcttcg atgcgttgcg ctcagcactc aaccccagag tgtagcttag gctgatgagt
     4141 ctggacatcc ctcaccggtg acggtggtcc aggctgcgtt ggcggcctac ctatggctaa
     4201 cgccatggga cgctagttgt gaacaaggtg tgaagagcct attgagctac ataagaatcc
     4261 tccggcccct gaatgcggct aatcccaacc tcggagcagg tggtcacaaa ccagtgattg
     4321 gcctgtcgta acgcgcaagt ccgtggcgga accgactact ttgggtgtcc gtgtttcctt
     4381 ttattttatt gtggctgctt atggtgacaa tcacagattg ttatcataaa gcgaattgga
     4441 ttgcggccgc actagtgatt agcttggata cacgccgccc acgtgaaggc tgccgacccc
     4501 gggatccacc ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     4561 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     4621 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     4681 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
     4741 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     4801 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     4861 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     4921 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     4981 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     5041 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     5101 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     5161 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     5221 tggacgagct gtacaagtaa agcggccgcc agcacagtgg tcgacgataa aataaaagat
     5281 tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt ttggcaagct
     5341 agcttaagta acgccatttt gcaaggcatg gaaaaataca taactgagaa tagagaagtt
     5401 cagatcaagg tcaggaacag atggaacagc tgaatatggg ccaaacagga tatctgtggt
     5461 aagcagttcc tgccccggct cagggccaag aacagatgga acagctgaat atgggccaaa
     5521 caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggtcccca
     5581 gatgcggtcc agccctcagc agtttctaga gaaccatcag atgtttccag ggtgccccaa
     5641 ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt ctcgcttctg
     5701 ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctca ctcggggcgc
     5761 cagtcctccg attgactgag tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt
     5821 tgcatccgac ttgtggtctc gctgttcctt gggagggtct cctctgagtg attgactacc
     5881 cgtcagcggg ggtctttcac atgcagcatg tatcaaaatt aatttggttt tttttcttaa
     5941 gtatttacat taaatggcca tagttgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     6001 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     6061 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     6121 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     6181 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     6241 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     6301 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     6361 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     6421 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     6481 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     6541 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     6601 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     6661 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     6721 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     6781 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     6841 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttgc
     6901 ggccggccgc aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     6961 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     7021 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     7081 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     7141 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     7201 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     7261 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     7321 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     7381 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     7441 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     7501 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     7561 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     7621 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     7681 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     7741 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     7801 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     7861 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttcc
//
