LOCUS       sequence-363566-        8514 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the fusion protein containing the extracellular part of 
            human Siglec-10 and the Fc region of human IgG1, and GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8514)
  AUTHORS   Alborzian Deh Sheikh A, Gomaa S, Li X, Routledge M, Saigoh K, Numoto
            N, Angata T, Hitomi Y, Takematsu H, Tsuiji M, Ito N, Kusunoki S, 
            Tsubata T
  TITLE     A Guillain-Barre syndrome-associated SIGLEC10 rare variant impairs 
            its recognition of gangliosides.
  JOURNAL   J Autoimmun. 2021 Jan;116:102571. doi: 10.1016/j.jaut.2020.102571. 
            Epub 2020 Nov 19.
  PUBMED    33223341
REFERENCE   2  (bases 1 to 8514)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Autoimmun."; date: "2021-01"; volume: "116:102571. doi"; pages: " 
            10.1016/j.jaut.2020.102571. Epub 2020 Nov 19"
COMMENT     Sequence Label: pMXs-Sig10-Fc-IG
FEATURES             Location/Qualifiers
     source          1..8514
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..615
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1094..1510
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1440..1462
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1478..1494
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1520..1894
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1832..1848
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     misc_feature    4565..5117
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4709..4726)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4936..4955
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5129..5131,5132..5134,5135..5848)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5129..5131,5132..5134,5135..5845)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5174..5195)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5435..5454)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5782..5803
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             6019..6488
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(6618..6635)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6789..7377)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6869..6888)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7541..8332,8333..8401))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8164..8183
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8402..8506)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
      541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
      601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
      661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
      781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
      841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
      901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct
      961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
     1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa
     1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca
     1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca
     1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg
     1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac
     1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc
     1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaaggat
     1921 cccagtgtgg tggtacggga attcgagctc ggtacctcgc gaatgcatct agagccacca
     1981 tgctactgcc actgctgctg tcctcgctgc tgggcgggtc ccaggctatg gatgggagat
     2041 tctggatacg agtgcaggag tcagtgatgg tgccggaggg cctgtgcatc tctgtgccct
     2101 gctctttctc ctacccccga caagactgga cagggtctac cccagcttat ggctactggt
     2161 tcaaagcagt gactgagaca accaagggtg ctcctgtggc cacaaaccac cagagtcgag
     2221 aggtggaaat gagcacccgg ggccgattcc agctcactgg ggatcccgcc aaggggaact
     2281 gctccttggt gatcagagac gcgcagatgc aggatgagtc acagtacttc tttcgggtgg
     2341 agagaggaag ctatgtgaga tataatttca tgaacgatgg gttctttcta aaagtaacag
     2401 ccctgactca gaagcctgat gtctacatcc ccgagaccct ggagcccggg cagccggtga
     2461 cggtcatctg tgtgtttaac tgggcctttg aggaatgtcc acccccttct ttctcctgga
     2521 cgggggctgc cctctcctcc caaggaacca aaccaacgac ctcccacttc tcagtgctca
     2581 gcttcacgcc cagaccccag gaccacaaca ccgacctcac ctgccatgtg gacttctcca
     2641 gaaagggtgt gagcgcacag aggaccgtcc gactccgtgt ggcctatgcc cccagagacc
     2701 ttgttatcag catttcacgt gacaacacgc cagccctgga gccccagccc cagggaaatg
     2761 tcccatacct ggaagcccaa aaaggccagt tcctgcggct cctctgtgct gctgacagcc
     2821 agccccctgc cacactgagc tgggtcctgc agaacagagt cctctcctcg tcccatccct
     2881 ggggccctag acccctgggg ctggagctgc ccggggtgaa ggctggggat tcagggcgct
     2941 acacctgccg agcggagaac aggcttggct cccagcagcg agccctggac ctctctgtgc
     3001 agtatcctcc agagaacctg agagtgatgg tttcccaagc aaacaggaca gtcctggaaa
     3061 accttgggaa cggcacgtct ctcccagtac tggagggcca aagcctgtgc ctggtctgtg
     3121 tcacacacag cagcccccca gccaggctga gctggaccca gaggggacag gttctgagcc
     3181 cctcccagcc ctcagacccc ggggtcctgg agctgcctcg ggttcaagtg gagcacgaag
     3241 gagagttcac ctgccacgct cggcacccac tgggctccca gcacgtctct ctcagcctct
     3301 ccgtgcacta ctccccgaag ctgctgggcc cctcctgctc ctgggaggct gagggtctgc
     3361 actgcagctg ctcctcccag gccagcccgg ccccctctct gcgctggtgg cttggggagg
     3421 agctgctgga ggggaacagc agccaggact ccttcgaggt cacccccagc tcagccgggc
     3481 cctgggccaa cagctccctg agcctccatg gagggctcag ctccggcctc aggctccgct
     3541 gtgaggcctg gaacgtccat ggggcccaga gtggatccat cctgcagctg ccagataagc
     3601 tcgagcccaa atcttgtgac aaaactcaca catgcccacc gtgcccaggt aagccagccc
     3661 aggcctcgcc ctccagctca aggcgggaca ggtgccctag agtagcctgc atccagggac
     3721 aggccccagc cgggtgctga cacgtccacc tccatctctt cctcagcacc tgaactcctg
     3781 gggggaccgt cagtcttcct cttcccccca aaacccaagg acaccctcat gatctcccgg
     3841 acccctgagg tcacatgcgt ggtggtggac gtgagccacg aagaccctga ggtcaagttc
     3901 aactggtacg tggacggcgt ggaggtgcat aatgccaaga caaagccgcg ggaggagcag
     3961 tacaacagca cgtaccgtgt ggtcagcgtc ctcaccgtcc tgcaccagga ctggctgaat
     4021 ggcaaggagt acaagtgcaa ggtctccaac aaagccctcc cagcccccat cgagaaaacc
     4081 atctccaaag ccaaaggtgg gacccgtggg gtgcgagggc cacatggaca gaggccggct
     4141 cggcccaccc tctgccctgg gagtgaccgc tgtaccaacc tctgtcccta cagggcagcc
     4201 ccgagaacca caggtgtaca ccctgccccc atcccgggat gagctgacca agaaccaggt
     4261 cagcctgacc tgcctggtca aaggcttcta tcccagcgac atcgccgtgg agtgggagag
     4321 caatgggcag ccggagaaca actacaaggc cacgcctccc gtgctggact ccgacggctc
     4381 cttcttcctc tacagcaagc tcaccgtgga caagagcagg tggcagcagg ggaacgtctt
     4441 ctcatgctcc gtgatgcatg aggctctgca caaccactac acgcagaaga gcctctccct
     4501 gtccccgggt aaatagcggc cgctacgtaa attccscccc ccycccnnnn nycccccccc
     4561 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta
     4621 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc
     4681 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat
     4741 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc
     4801 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt
     4861 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt
     4921 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag
     4981 aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt
     5041 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga
     5101 aaaacacgat gataatatgg ccacaaccat ggtgagcaag ggcgaggagc tgttcaccgg
     5161 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     5221 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     5281 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
     5341 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     5401 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     5461 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     5521 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     5581 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     5641 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     5701 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
     5761 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     5821 tctcggcatg gacgagctgt acaagtaagt cgacgataaa ataaaagatt ttatttagtc
     5881 tccagaaaaa ggggggaatg aaagacccca cctgtaggtt tggcaagcta gcttaagtaa
     5941 cgccattttg caaggcatgg aaaaatacat aactgagaat agagaagttc agatcaaggt
     6001 caggaacaga tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct
     6061 gccccggctc agggccaaga acagatggaa cagctgaata tgggccaaac aggatatctg
     6121 tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtcca
     6181 gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat
     6241 gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt
     6301 ctgctccccg agctcaataa aagagcccac aacccctcac tcggggcgcc agtcctccga
     6361 ttgactgagt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact
     6421 tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg
     6481 gtctttcaca tgcagcatgt atcaaaatta atttggtttt ttttcttaag tatttacatt
     6541 aaatggccat agttgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     6601 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     6661 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     6721 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     6781 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     6841 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     6901 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     6961 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     7021 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     7081 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     7141 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     7201 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     7261 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     7321 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     7381 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     7441 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttgcg gccggccgca
     7501 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     7561 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     7621 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     7681 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     7741 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     7801 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     7861 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     7921 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     7981 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     8041 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     8101 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     8161 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     8221 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     8281 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     8341 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     8401 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     8461 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttc
//
