LOCUS       sequence-36357-e         708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Zhu C, Smith T, McNulty J, Rayla AL, Lakshmanan A, Siekmann AF, 
            Buffardi M, Meng X, Shin J, Padmanabhan A, Cifuentes D, Giraldez AJ,
            Look AT, Epstein JA, Lawson ND, Wolfe SA
  TITLE     Evaluation and application of modularly assembled zinc-finger 
            nucleases in zebrafish.
  JOURNAL   Development. 2011 Oct;138(20):4555-64.
  PUBMED    21937602
REFERENCE   2  (bases 1 to 708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2011-10"; volume: "138(20)"; pages: "4555-64"
COMMENT     Sequence Label: pCS2-F1-GGC
FEATURES             Location/Qualifiers
     source          1..708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      6..15
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             18..38
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             39..104
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 gatctgccac catggctcca aagaagaagc gtaaggtaga ctacaaagac catgacggtg
       61 attataaaga tcatgacatc gattacaagg atgacgatga caagggtacc cgcccatatg
      121 cttgccctgt cgagtcctgt gaccgccgct tcagcgagaa gtctcacctc acccgccata
      181 tccggattca cacaggccag aagcccttcc agtgtcgcat ctgcatgagg aacttcagcc
      241 agaattgtca cctcacccgc cacatccgca cccacacagg cgagaagcct tttgcgtgcg
      301 acatttgcgg caggaagttc gcgcttaggc atcacctcaa gcagcacacc aaaatccata
      361 caggtggatc ccaactagtc aaaagtgaac tggaggagaa gaaatctgaa cttcgtcata
      421 aattgaaata tgtgcctcat gaatatattg aattaattga aattgccaga aattccactc
      481 aggatagaat tcttgaaatg aaggtaatgg aattttttat gaaagtttat ggatatagag
      541 gtaaacattt gggtggatca aggaaaccgg acggagcaat ttatactgtc ggatctccta
      601 ttgattacgg tgtgatcgtg gatactaaag cttatagcgg aggttataat ctgccaattg
      661 gccaagcaga tgaaatgcaa gactatgtcg aagaaaatca aacacgaa
//
