LOCUS       sequence-36358-e         999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 999)
  TITLE     george cross lab plasmid
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ZM (pyrFEKO-HYG TetR-T7RNAP)
FEATURES             Location/Qualifiers
     source          1..999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    583..616
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ctgagctgca gggggctcga atccccccat tttcttcttt tacatcatcc ccccttccta
       61 accaacctgc aggcagctga catgtaaagg atgtaaacga gaaatgaggg aaaggtgatg
      121 gctccgagtg atggaacttc atacatccca gatacccttc aatgcagagg gcaccacagc
      181 aaattgatca actgctacct tttttttatt gctgctctac agttattaga aaccctcact
      241 tcgctatcac ctcctcaacc acacacaaat acctttgaag cgattaaacc ttccctccca
      301 aaccccattg ctgtctcata tagtttacta cttgctttat tattattatt aaaaacttgc
      361 cttcgccact tagttaaacg ctgcgaccgt aaaagtgcat ttcatggtcc atcaatttaa
      421 agtgaattgt tcattcacca aactgtttaa attcatggta gggagcatta ttattattcc
      481 ccacgcttac gatctgaagt aggtacgaaa ataatttgca acgaaaacat gtgtgtacgt
      541 ccgcaatagc ttaaagatag agctccgacc cacttaggat ccataacttc gtataatgta
      601 tgctatacga agttatgaat tctcagttag cctcccccat ctcccgggca aacgtgcgcg
      661 ccaggtcgca tatcgtcggt atggagccgg gggtggtgac gtgggtctgg accatcccgg
      721 aggtaagttg cagcagggcg tcccggcagc cggcgggcga ttggtcgtaa tccaggataa
      781 agacgtgcat ggaacggagg cgtttggcca agacgtccaa ggcccaggca aacacgttat
      841 acaggtcgcc gttgggggcc agcaactcgg gggcccgaaa cagggtaaat aacgtgtccc
      901 cgatatgggg tcgtgggccc gcgttgctct ggggctcggc accctggggc ggcacggcng
      961 tccccgaaag tgccngctcg cncacgcccg gacgcgtga
//
