LOCUS       sequence-363616-        9531 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMV and T7 promoter expression plasmid for human codon usage 
            anCas(BCA) with an N-terminal NLS and C-terminal NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9531)
  AUTHORS   Alonso-Lerma B, Jabalera Y, Samperio S, Morin M, Fernandez A, Hille 
            LT, Silverstein RA, Quesada-Ganuza A, Reifs A, Fernandez-Penalver S,
            Benitez Y, Soletto L, Gavira JA, Diaz A, Vranken W, Sanchez-Mejias 
            A, Guell M, Mojica FJM, Kleinstiver BP, Moreno-Pelayo MA, Montoliu 
            L, Perez-Jimenez R
  TITLE     Evolution of CRISPR-associated endonucleases as inferred from 
            resurrected proteins.
  JOURNAL   Nat Microbiol. 2023 Jan;8(1):77-90. doi: 10.1038/s41564-022-01265-y.
            Epub 2023 Jan 2.
  PUBMED    36593295
REFERENCE   2  (bases 1 to 9531)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-022-01265-y"; journalName: "Nat Microbiol"; date: 
            "2023-01"; volume: "8"; issue: "1"; pages: "77-90"
COMMENT     Sequence Label: pCMV-T7-NLS(SV40)-anCas(BCA)-6xHis-NLS(SV40)
FEATURES             Location/Qualifiers
     source          1..9531
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(72..91)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        263..642
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        643..846
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     797..817
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     891..910
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        891..909
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             938..958
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4985..5002
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5021..5041
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5150..5167)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5156..5380
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5426..5854
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5513..5532)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5723..5744
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5863..5883)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5868..6197
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6048..6183
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6110..6129
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6264..7058
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6318..6337)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6928..6947
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7232..7353
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7269..7288)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7323..7342
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7402..7418)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7402..7418)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7415..7437)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7426..7442
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7450..7456,7457..7474,7475..7480))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7495..7516
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7633..7650)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7804..8392)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7884..7903)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8563..9354,9355..9423))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9186..9205
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9424..9528)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg
       61 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct
      121 tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc
      181 ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat
      241 gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt
      301 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
      361 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
      421 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
      481 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
      541 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
      601 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
      661 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
      721 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
      781 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
      841 agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact
      901 cactataggg agacccaagc tggctagcgc caccatgccc aagaaaaagc ggaaggtgcc
      961 caagatgaag aaggactatt ctatcggact ggacatcggc acaaacagcg tgggctgggc
     1021 cgtgatcaca gacgactaca aggtcgtgcg caagaaaatg aaggtgctgg gcaacaccga
     1081 taaaaagagc atcaagaaaa acctgtgggg agtgagactg ttcgactctg gcgaaaccgc
     1141 cgaggataca agactgaagc gaaccaccag acggagatac accagaagaa gaaaccgcat
     1201 ctgctacctg caggaaatct tccaggaaga gatgaacaag gtcgacgaca gcttcttcca
     1261 ccgcctggat gaaagcttcc tggtgcccga ggataaaaag tacgacagac accctatctt
     1321 tggcaacctg gaagaagaag tggcctacca tgagcaatac cctaccatct accacctgcg
     1381 gaagcacctc gccgattcta gtgagaaggc cgatctgaga ctggtttacc tggccctggc
     1441 ccatatcatc aaatacagag gccacttcct gatcgaaggc gacctgaaca ccgagaacac
     1501 tgacgtggaa gagctgttca aacagttcgt gcaggtgtac aaccagacct tcgaggaaca
     1561 acacctgtcg gatgagacca tcgacgtgga ggaaatcctg acagaaaaag tgtccaagag
     1621 cagacgggcc gagaacgtgc tgaagctatt tccaaatgag aagaaaaacg gcctgtttgg
     1681 ccagctgatc aagctgatcg ttggcctgca gggcaacttc aagaaagtgt tcgacctcag
     1741 cgaggacgcc aagctccaat tttctaagga tacctatgag gaggacctgg aaaaccttct
     1801 ggccatgatc ggggacgagt acgccgacct gttcctggcc gccaaaaacc tgtacgacgc
     1861 tatcctgctt tctggaatcc tgaccacaac cgataataac accaaggcca agctgagcgc
     1921 cagcatgatc aagcggtacg aggagcacca gaaggacctg gcacaactga agcagttcat
     1981 caaagagcac ctgccagaca agtacaatga aatcttcagc gactccagca aaaacggcta
     2041 tgccggctac atcgagggca aaaccagcca ggaggatttc tacaagtacc tgaagaagat
     2101 cctgtctaag attgatggcg ctgaatactt cctggagaaa attgaacagg aaaattttct
     2161 gaggaagcaa agaacctttg acaacggcgt gatcccgcac cagatccacc tggaggagct
     2221 gagagccatc ctgaggcgtc agggcaagta ctacccattc ctcaaagaga accaggagaa
     2281 gatagagcag atcctgacat ttagaatccc ctactacgtg ggcccactgg ctagaggaaa
     2341 tagccggttc gcctggctga cacggaagtc tgacgagccc atcacacctt ggaacttcga
     2401 cgaagtcgtg gacaaggaga agtccgccga ggccttcatc gagagaatga ccaactacga
     2461 cacctacctg cctaacgaaa aggtactgcc caaacacagc ctgctgtacg agaagttcac
     2521 cgtgttcaac gaattgacaa aggttcggta cgtgacagac cggggcaaga cccagaactt
     2581 ctccgccgag cagaagcagg agatcttcga cgacctgttc aagaagaacc ggaaggtcac
     2641 aaagaaaaag ctggagaact atctgaagaa agaatacgag tacttcgaca gccctgatat
     2701 caccggaatt gaggacgagt tcaacgccag cctgggcacc tatcatgatc tgctgaagat
     2761 cctgaagtct aaggacttcc tggacgatga ggaaaacgag gaaatcctgg aggatatcgt
     2821 gaagatcctg accgtgttcg aggacagaaa gatgatcaga aagcggctgg agaaatacag
     2881 cgacatcctc accgaggaac agttgaagaa gctggagcgg cggcactaca caggctgggg
     2941 cagattgagc agaaagctta tcaacggcat ccgcgacaaa cagagcggca agacaatttt
     3001 agattacctg atcgacgacg acagcagtaa ccggaacttc atgcagctga tcaacgatga
     3061 caatttgagc tttaaggagg aaatcgagaa ggcccaggtg atcggagaga ccgagaacct
     3121 gcacgagata gtgcaagacc tgcctggcag ccctgctatc aagaagggca tcctgcagtc
     3181 tctgaagatt gtggacgagc tcgtgaaggt gatggggcac gaacctaaga acatcgtggt
     3241 cgaaatggcc agagagaacc agacgaccag caggggcaga cggaacagca agcagcggct
     3301 gaagagactg gaggaggctc ttaagaacct gggcagcaac ctgctgaaag aacaccccgt
     3361 ggacaatcag cagctgcaga acgacagact gtacctatac tacctgcaaa atggcaagga
     3421 tatgtacaca ggacaggagc tggacatcga taatctgtcc aactacgaca tcgatcacat
     3481 catcccccag agtttcatca aggataatag catcgataac cgagtgctgg tgagcagcga
     3541 ggaaaataga ggcaagtctg ataatgtgcc ttctaaggaa gttgtgagaa agatgaaatc
     3601 tttttggcag aaactgctga atgctggcct gatcagccag agaaaattcg acaacctgac
     3661 aaaggctgag agaggcggcc tgaccgaaga cgacaaagct ggattcatca agagacagct
     3721 ggtggaaaca agacagatca ccaagcacgt ggccaacatc ctagacagcc ggttcaacac
     3781 agagaaggac gaggaggaca acaccatcag aaacgtgaag attatcaccc tgaagtccaa
     3841 cctggtgtcc cagttcagaa aggatttcgg cctgtacaag gttcgggaaa ttaacgacta
     3901 ccaccacgcc cacgacgcct acctgaatgc cgtggtgggc accgccctgc tgaagaagta
     3961 tcctcaactg gagcctgagt tcgtgtacgg cgactaccgg aagaacaacg ccagagagga
     4021 aaacaaggcc acagccaaga agcacttcta cagcaatatc atgaacttct tcgcctctga
     4081 agtgaagatt gccgacgaaa ccggcgagat cgtgtgggac aaagaaaagg acatagctac
     4141 cgtgcggaag gtgctgtcct accaccaggt gaacatcgtg aagaaagtgg aggtgcagaa
     4201 gggaggattc tccaaggaaa ccatcctccc caagggcaac agcaacaagc tcatccccag
     4261 aaagaacaac tgggacccta agaagtacgg cggcttcgac agccccaccg tggcttatag
     4321 cgtgctggtg acctacgata tcgagaaggg aaagaaaaag aagctgaaga ccgtgaaaga
     4381 gctggtcggc atcaccatca tggagcggag cgcctttgaa aagaacccca tcgcctacct
     4441 ggaaaaaaaa ggctaccaga atcctcagga ggacgtgctg atcaaactgc ctaagtactc
     4501 cctgttcgag ctggaaaacg gcagacgtcg gatgctggcc agcgctaagg aactccaaaa
     4561 gggcaatcag atggtgcttc ctgcccatct ggtgaccctg ctgtaccacg ccaaacggat
     4621 cgataagagc aacaattctg agagcctgga atacgtggaa gaacacagaa atgaattcga
     4681 cgagctgctg gactatatca ttgatttcag cgagaagtat atcctggctg ataagaacct
     4741 ggaaaagatc aagaagcttt acgagcagaa caacgaggcc gacatcaagg agctggccaa
     4801 gagctttatc aacctgctga ccttcaccgc catgggcgcc cctgccgctt tcaagttctt
     4861 cggcgaaaca atcgacagaa aaagatacac aagcatcaag gaactgctca acgccaccct
     4921 catccaccag agcatcacag gtctgtacga gacaagaatc gacctgggca agctgggaga
     4981 ggatcaccat catcatcacc acgcctctgg aacaggcgga cctaaaaaga agaggaaggt
     5041 ttaaggtacc gagctcggat ccactagtcc agtgtggtgg aattctgcag atatccagca
     5101 cagtggcggc cgctcgagtc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg
     5161 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     5221 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     5281 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     5341 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     5401 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     5461 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     5521 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     5581 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     5641 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     5701 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     5761 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     5821 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
     5881 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     5941 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     6001 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     6061 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     6121 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     6181 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag
     6241 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
     6301 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     6361 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     6421 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga
     6481 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     6541 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     6601 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     6661 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     6721 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     6781 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     6841 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     6901 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     6961 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     7021 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
     7081 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
     7141 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     7201 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     7261 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     7321 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     7381 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     7441 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     7501 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     7561 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     7621 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     7681 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     7741 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     7801 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     7861 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     7921 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     7981 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     8041 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     8101 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     8161 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     8221 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     8281 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     8341 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     8401 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     8461 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     8521 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     8581 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     8641 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     8701 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     8761 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     8821 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     8881 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     8941 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     9001 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     9061 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     9121 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     9181 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     9241 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     9301 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     9361 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     9421 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     9481 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca c
//
