LOCUS       sequence-363626-        9576 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMV and T7 promoter expression plasmid for human codon usage 
            anCas(PCA) with an N-terminal NLS and C-terminal NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9576)
  AUTHORS   Alonso-Lerma B, Jabalera Y, Samperio S, Morin M, Fernandez A, Hille 
            LT, Silverstein RA, Quesada-Ganuza A, Reifs A, Fernandez-Penalver S,
            Benitez Y, Soletto L, Gavira JA, Diaz A, Vranken W, Sanchez-Mejias 
            A, Guell M, Mojica FJM, Kleinstiver BP, Moreno-Pelayo MA, Montoliu 
            L, Perez-Jimenez R
  TITLE     Evolution of CRISPR-associated endonucleases as inferred from 
            resurrected proteins.
  JOURNAL   Nat Microbiol. 2023 Jan;8(1):77-90. doi: 10.1038/s41564-022-01265-y.
            Epub 2023 Jan 2.
  PUBMED    36593295
REFERENCE   2  (bases 1 to 9576)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-022-01265-y"; journalName: "Nat Microbiol"; date: 
            "2023-01"; volume: "8"; issue: "1"; pages: "77-90"
COMMENT     Sequence Label: pCMV-T7-NLS(SV40)-anCas(PCA)-6xHis-NLS(SV40)
FEATURES             Location/Qualifiers
     source          1..9576
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(40..59)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        231..610
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        611..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     765..785
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     859..878
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        859..877
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             928..948
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5059..5076
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5095..5115
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5163..5180)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5169..5393
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5439..5867
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5526..5545)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5736..5757
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5876..5896)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5881..6210
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6061..6196
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6123..6142
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6277..7071
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6331..6350)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6941..6960
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7245..7366
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7282..7301)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7336..7355
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7415..7431)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7415..7431)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7428..7450)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7439..7455
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7463..7469,7470..7487,7488..7493))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7508..7529
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7646..7663)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7817..8405)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7897..7916)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8576..9367,9368..9436))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9199..9218
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9437..9541)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc
       61 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag
      121 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
      181 ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt
      241 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
      301 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      361 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      421 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      481 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      541 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      601 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      661 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      721 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      781 gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc
      841 ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg gctagcgttt
      901 aaacttaagc ttggtaccgc caccatgccc aagaaaaagc ggaaggtgcc caagatgaag
      961 aaaccataca gcatcggcct ggacatcggc accaacagcg tgggctgggc cgtgatcaca
     1021 gatgattaca aggtgcccgc taagaagatg aaggtgctgg gcaacaccga cagacaaagt
     1081 attaagaaaa acctgatcgg agccctgctg tttgacagcg gagagacagc tgaggccacc
     1141 agactgaaac ggacagctcg tagaaggtat accagaagaa agaacagaat ctgttacctg
     1201 caggagatct ttagcgaaga gatggccaag gtggacgaca gcttctttca ccgcctggaa
     1261 gaatctttcc tggtgcccga ggataagaag tacgatagac accctatctt cggcaatctg
     1321 gcggatgaag tggcctacca cgaaaactac cccaccatct accacctgag aaagaagctg
     1381 gccgactcta cagagaaagc cgacctgaga ctgatctacc tggcccttgc ccatatcatc
     1441 aagttccggg gccacttcct gatcgagggc gaccttaacg ccgaaaacac cgacgtgcag
     1501 aaactgttcc accagctggt ggatacctac aaccagctgt tcgaggagga ccagctggat
     1561 actgagacca tcgacgcaaa ggccatcctg accgctaaga ttagcaagag cagacgcctg
     1621 gaaaacctga tcagccaaat ccctggacag aaaaaaaacg gcctgtttgg caacctgatc
     1681 gccctgagcc tgggcctgac ccctaatttc aagtctaact tcgacctgtc cgaggatgcc
     1741 aaactgcagc tgtccaagga cacctacgag gaggatctgg acaatctgct tgctcagatc
     1801 ggcgatcagt acgccgacct atttctcgcc gccaagaacc tgagcgatgc cattctgctc
     1861 tccgacatct tgacagtcaa tgacgagagc acaaaagccc ctctgtctgc ttccatgatc
     1921 aagcggtacg aagaacacca gcaggacctg gccctgctga aacagctggt gaaggaacag
     1981 ctgcccgaga aatacaaaga gatcttctct gacaaatcta agaacggata cgccggatat
     2041 atcgatggca agacctccca ggaggagttc tacaagtaca tcaaacctat cctgtccaag
     2101 ctcgatggcg ccgaggaatt ccttgccaag atcgatagag aagattttct gcgcaagcag
     2161 agaacctttg acaacggctc catcccccac cagatccacc tcgaagagct gcatgcgatc
     2221 ctgagaagac aggaggaata ctacccgttc ctgaaggaca atcaagagaa gatcgaaaaa
     2281 atattgacct tccgaatccc atactacgtg ggacctctgg ccagaggaaa tagcagattc
     2341 gcctggctga ccagaaaatc tgatgaggcc atcacaccct ggaactttga ggaggtcgtg
     2401 gataaagaag cctccgccca ggcgttcatc gagcgtatga caaacttcga tacgtacctg
     2461 cccaacgaaa aggtgcttcc aaaacattct ctgctgtacg agaccttcac agtgtacaac
     2521 gagttgacaa aagtgaagta cgtgaccgag ggcatgacca agcctcaatt tctcagcgcg
     2581 gagcagaagc aagccatcgt ggacctgctg ttcaagaaga accggaaggt gacagtgaaa
     2641 cagcttaagg aagactattt caagaagatc gagtgcttcg actccgtgga catcacagga
     2701 gttgaagaca ggttcaacgc cagcctgggc acataccacg atctgctgaa gatcatcaag
     2761 gacaaggact tcctggacaa cgaggagaat gaagatatcc tggaagatat cgtactgaca
     2821 ctgacactgt tcgaggatag agagatgatc gaaaagagac tggccaagta cgcggacctg
     2881 ttcgacaaga aggtgctgaa gaaactgaag agacggcact acaccggatg gggcagactg
     2941 tcccggaaac tgatcaatgg aatcagagat aagcagagcg gcaagaccat cctggatttt
     3001 ctgaaggccg atggattcgc caatagaaac tttatgcagc tgattaacga cgattccctg
     3061 agctttaagg aggaaatcga gaaggcccaa gtgatcggcc agaccgactc cctgcacgag
     3121 gtggtggccg acttagctgg cagccccgcc atcaagaagg gcatcctgca gacaatcaag
     3181 atcgtggacg agctggtgaa ggttatgggc cggcacaacc ccgaaaatat cgtcatcgaa
     3241 atggccagag agaaccagac caccgcccaa ggcatcaaga atagtagaca gcggatgaag
     3301 cggctggagg aggtgctgaa gaaactgggc agcaacatcc tgaaggagca ccccgtggac
     3361 aacacccagc tgcagaacga caggctgtac ctgtactacc tgcaaaatgg caaggacatg
     3421 tacaccggcc aggagctgga cattgacaac ctcagccagt acgacattga ccacatcatc
     3481 cctcagagtt tcatcaagga caacagcatc gacaacaagg tcctcaccag cagcgaggaa
     3541 aacagaggca agagcgacaa tgtgcctagc atcgaggtgg tgcggaagat gaagagctac
     3601 tggcagaaac tgctgaacgc cggcctgatt tctcagcgaa agttcgataa tctgaccaag
     3661 gcagagcggg gtggactgac tgagagcgac aaagctggct tcatcaagcg gcagctggtc
     3721 gagacaagac agatcaccaa gcacgtggcc caaatcctgg acagcagatt caacactgag
     3781 cgggacgaaa acgacaaacc tatcagaaac gtgaagatca tcaccctgaa gtctaagctg
     3841 gtgtcggact tcagaaaaga cttcggcctg tacaaggtta gagagattaa cgattaccac
     3901 cacgcccacg acgcctacct gaacgccgtg gtcggaacag ccctgctgaa gaagtaccct
     3961 aagctggagc ctgagttcgt gtatggcgac tacaaaaaat atgatctgag aaagctgatc
     4021 gccaagagcg acaaggagag aggcaaggcc acagcaaaga tgttcttcta cagcaacatc
     4081 atgaacttct tcaagacaga agtgaagctg gctaatggca caatcatcaa gagacctgtg
     4141 atcgagacca acgaggaaac cggcgagatc gtgtgggaca aggaaaagga tttcgccacc
     4201 gtgcggaagg tgctgagcta cccccaggtg aacatcgtca agaagaccga ggtgcagacc
     4261 ggcggcttca gcaaggaaag catcctccct aagggcaaca gcgacaagct aatcccaaga
     4321 aagaacaact gggaccctaa gaagtacggc ggattcgact ctcctaccgt ggcctatagc
     4381 gtgctggttg tggccaaggt ggaaaagggc aaggccaaga agctgaagac cgtgaaagaa
     4441 ctggtgggca tcaccatcat ggaaagaagc gccttcgaaa agaatcctat tgcctttctc
     4501 gaagctaagg gctaccagga catccaggag gacctgataa tcaaactgcc taaatacagc
     4561 ctgttcgagc tggagaacgg ccggagacgg ctcctggcta gcgctaaaga gctacagaag
     4621 ggcaacgaga tggtgctgcc tgcccacctg gtgaccttcc tgtaccatgc cagccggatc
     4681 gataaaagca cgggctctag cgaaaactta gagcaaaagc aggagtacgt ggaacagcac
     4741 aagcacgagt tcgatgagat cctggactac atcattgact tttccgagag atacatcctg
     4801 gccgataaga acctggagaa gatcaaaagc ctgtataacc agaacgacga ctccgacatc
     4861 aacgagttgg cctcttcttt catcaatctg ttcaccttca ccgccctggg cgcccctgct
     4921 gcctttaagt tcttcgacgc tacaatcgat agaaagcggt ataccagcac caaggaggtg
     4981 ctgaatgcta cactgatcca ccagagcatc accggactgt acgagactag aatcgacctt
     5041 tcccagttag gcggcgatca ccatcatcat caccacgcct ctggaacagg cggacctaaa
     5101 aagaagagga aggtttaagc ggccgctcga gtctagaggg cccgtttaaa cccgctgatc
     5161 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     5221 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     5281 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     5341 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     5401 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     5461 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     5521 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     5581 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     5641 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     5701 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     5761 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     5821 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
     5881 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     5941 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     6001 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     6061 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     6121 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     6181 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     6241 ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     6301 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     6361 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     6421 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
     6481 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     6541 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     6601 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     6661 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     6721 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     6781 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat
     6841 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     6901 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     6961 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     7021 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
     7081 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
     7141 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     7201 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     7261 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     7321 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     7381 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     7441 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     7501 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     7561 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     7621 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     7681 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     7741 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     7801 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     7861 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     7921 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     7981 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     8041 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     8101 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     8161 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     8221 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     8281 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     8341 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     8401 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     8461 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     8521 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     8581 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     8641 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     8701 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     8761 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     8821 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     8881 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     8941 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     9001 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     9061 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     9121 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     9181 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     9241 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     9301 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     9361 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     9421 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     9481 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     9541 gcacatttcc ccgaaaagtg ccacctgacg tcgacg
//
