LOCUS       sequence-363627-        4256 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Subcloning YAP1 WT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Hatterschide J, Castagnino P, Kim HW, Sperry SM, Montone KT, Basu D,
            White EA
  TITLE     YAP1 activation by human papillomavirus E7 promotes basal cell 
            identity in squamous epithelia.
  JOURNAL   Elife. 2022 Feb 16;11. pii: 75466. doi: 10.7554/eLife.75466.
  PUBMED    35170430
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-02-16"; volume: "11. pii: 75466. doi"; pages: " 
            10.7554/eLife.75466"
COMMENT     Sequence Label: p8319 pDONR ATG-YAP1 WT-Stop
FEATURES             Location/Qualifiers
     source          1..4256
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      79..106
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(265..282)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(436..1024)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(516..535)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1117..1908)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     2339..2355
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2339..2355
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2360..2379
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2360..2378
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2396..2495
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3962..4061)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4077..4094)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4077..4093)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4086..4108)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      4157..4243
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 atttgaacgt tgtgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg
       61 ccaggcatca aactaagcag aaggccatcc tgacggatgg cctttttgcg tttctacaaa
      121 ctcttcctgg ctagcggtac gcgtattaat tgcgttgcgc tcactgcccg ctttccagtc
      181 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
      241 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
      301 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      361 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      421 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      481 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      541 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      601 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      661 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      721 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
      781 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
      841 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
      901 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
      961 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     1021 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg acgcgtaact
     1081 cacgttaagg gattttggtc atgggtggct cgagggttat ttgccgacta ccttggtgat
     1141 ctcgcctttc acgtagtgga caaattcttc caactgatct gcgcgcgagg ccaagcgatc
     1201 ttcttcttgt ccaagataag cctgtctagc ttcaagtatg acgggctgat actgggccgg
     1261 caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc cggttactgc
     1321 gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcgccag cccagtcggg
     1381 cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct gttcaggaac
     1441 cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt ctcttgcttt
     1501 tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac ctgcaagaat
     1561 gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac gccacggaat
     1621 gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc tctctccagg
     1681 ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt catcaagcct
     1741 tacggtcacc gtaaccagca aatcaatatc actgtgtggc ttcaggccgc catccactgc
     1801 ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga tgacgccaac
     1861 tacctctgat agttgagtcg atacttcggc gatcaccgct tccctcataa tgtttaactt
     1921 tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg
     1981 acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta ccccaaaaaa
     2041 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     2101 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagcttac gaaccgaaca
     2161 ggcttatgtc cactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
     2221 cttgggtagc agcgaagtcg aggcatttct gtcctggctg gtctagagga ggcatgatga
     2281 tatattttta tcttgtgcaa tgtaacatca gagattttga gacacgggcc agagctgcca
     2341 ggaaacagct atgaccatgt aatacgactc actatagggg mtatcagctg gatggcaaat
     2401 aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgata agcaatgctt
     2461 tcttataatg ccaactttgt acaagaaagt tgggtttata accatgtaag aaagctttct
     2521 ttatctagct tggtggcagc caaaacagac tccatgtcat taaggatgtc agaactcaaa
     2581 gcttcctgca gacttggcat cagctcctct ccttctatgt tcattccatc tccttccagt
     2641 gttccaaggt ccacatttgt cccaggaatg gcttcaaggt agtctgggaa acggttctgc
     2701 tgtgagggca gggtgctttg gttgatagta tcacctgtat ccatctcatc cacactgttc
     2761 aggaagtcat ctggggttcg agggacactg tagctgctca tgcttagtcc actgtctgta
     2821 ctctcatctc gagagtgata ggtgccactg ttaaggaaag gatctgagct attggtcgtc
     2881 attgttctca attcctgaga catcccggga gaagacactg gattttgagt cccaccatcc
     2941 tgctccagtg ttggtaactg gctacgcagg gctaactcct gccgaagcag ttcttgctgt
     3001 ttcagccgca gcctctcctt ctccatctgc agttgctgca gtcgcatctg ttgctgctgg
     3061 ttggagttgc tgccacccat gacgcctccc tgtgggctct ggggagccag gggtggtggc
     3121 tgtttcactg gagcactctg actgattctc tggttcatgg caaaacgagg gtcaagcctt
     3181 gggtctagcc aagaggtggt cttgttctta tggtttatat agtaaatttc tccatcctga
     3241 gtcatggctt gttcccatcc atcaggaaga ggacctgaag ccgagttcat catattctgc
     3301 tgcactggtg gactggtggg ggctgtgacg ttcatctggg acagcatggc cttcctgggg
     3361 tcctgccatg ttgttgtctg atcgatgtga tttaagaagt atctctgacc agaagatgtc
     3421 tttgccatct cccaacctgc tggcagaggt acatcatcag gtatctcaaa agaagactgt
     3481 cgaagatgct gagctgtggg tgtagctgct gggccagaga ctactccagt gggggtcagt
     3541 gtcccaggag aaacagctcc caactgcaga gaagctggag aggaatgagc tcgaacatgc
     3601 tgtggagtca gggctcctgc agtgcctgca tcagtactgg cctgtcggga gtgggatttg
     3661 ggctccggcg gcttgaagaa ggagtcgggc agcttccgga gcctcatggg cacggtctgg
     3721 ggcacgttgg ccgtcttggg gttcatgacg gcgttgaaga gcgcctccag gtcggtctcc
     3781 gagtccccgc ggacgtgcac gatctgatgc ccggcggggg gtgcctgcgg cgccgcctgg
     3841 gtcgccgcgg gtgccggttg cccgggtccg gacggcgggc cctgcccctg cgggggctgc
     3901 gaaggcggct gcccttggcc ctggggggcc ggttgaggcg gcggctgctg cccgggatcc
     3961 atgccaactt ttttgtacaa agttggcatt ataaaaaagc attgctcatc aatttgttgc
     4021 aacgaacagg tcactatcag tcaaaataaa atcattattt ggggcccgag cttaagactg
     4081 gccgtcgttt tacaacgtcg tgactgggaa aacatccatg ctagcgttaa cgcgagagta
     4141 gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg gaagactggg cctttcgttt
     4201 tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcgg
//
