LOCUS       sequence-363628-        9615 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMV and T7 promoter expression plasmid for human codon usage 
            anCas(SCA) with an N-terminal NLS and C-terminal NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9615)
  AUTHORS   Alonso-Lerma B, Jabalera Y, Samperio S, Morin M, Fernandez A, Hille 
            LT, Silverstein RA, Quesada-Ganuza A, Reifs A, Fernandez-Penalver S,
            Benitez Y, Soletto L, Gavira JA, Diaz A, Vranken W, Sanchez-Mejias 
            A, Guell M, Mojica FJM, Kleinstiver BP, Moreno-Pelayo MA, Montoliu 
            L, Perez-Jimenez R
  TITLE     Evolution of CRISPR-associated endonucleases as inferred from 
            resurrected proteins.
  JOURNAL   Nat Microbiol. 2023 Jan;8(1):77-90. doi: 10.1038/s41564-022-01265-y.
            Epub 2023 Jan 2.
  PUBMED    36593295
REFERENCE   2  (bases 1 to 9615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-022-01265-y"; journalName: "Nat Microbiol"; date: 
            "2023-01"; volume: "8"; issue: "1"; pages: "77-90"
COMMENT     Sequence Label: pCMV-T7-NLS(SV40)-anCas(SCA)-6xHis-NLS(SV40)
FEATURES             Location/Qualifiers
     source          1..9615
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(73..92)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        264..643
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        644..847
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     798..818
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     892..911
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        892..910
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             939..959
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5070..5087
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5106..5126
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5235..5252)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5241..5465
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5511..5939
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5598..5617)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5808..5829
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5948..5968)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5953..6282
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6133..6268
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6195..6214
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6349..7143
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6403..6422)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7013..7032
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7317..7438
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7354..7373)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7408..7427
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7487..7503)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7487..7503)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7500..7522)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7511..7527
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7535..7541,7542..7559,7560..7565))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7580..7601
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7718..7735)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7889..8477)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7969..7988)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8648..9439,9440..9508))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9271..9290
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9509..9613)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg
       61 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
      121 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
      181 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga
      241 tgtacgggcc agatatacgc gttgacattg attattgact agttattaat agtaatcaat
      301 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
      361 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
      421 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
      481 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
      541 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
      601 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
      661 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
      721 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
      781 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
      841 cagagctctc tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
      901 tcactatagg gagacccaag ctggctagcg ccaccatgcc caagaaaaag cggaaggtgc
      961 ccaagatgaa gaagccctac agcatcggcc tggacatcgg caccaacagc gtgggctggg
     1021 ccgtgatcac cgacgactac aaggtgcccg ccaagaagat gaaggtgctg ggcaacaccg
     1081 acaagcagag catcaagaag aacctgctgg gcgccctgct gttcgacagc ggcgagaccg
     1141 ccgaggccac caggctgaag aggaccgcca ggaggaggta caccaggagg aggaacagga
     1201 tctgctacct gcaggagatc ttcagcgagg agatgaacaa ggtggacgac agcttcttcc
     1261 acaggctgga cgagagcttc ctggtgcccg aggacaagaa gtacgacagg caccccatct
     1321 tcggcaacct ggccgaggag gtggcctacc acgagcagta ccccaccatc taccacctga
     1381 ggaagcacct ggccgacagc accgagaagg ccgacctgag gctggtgtac ctggccctgg
     1441 cccacatcat caagttcagg ggccacttcc tgatcgaggg cgacctgaac gccgagaaca
     1501 ccgacgtgca gaagctgttc cagcagttcg tggaggtgta caaccagacc ttcgaggaga
     1561 gccacctgag cgaggagacc atcgacgtgg aggagatcct gaccgagaag atcagcaaga
     1621 gcaggaggct ggagaacctg atcaagcact tccccaacga gaagaagaac ggcctgttcg
     1681 gcaacctgat cgccctgatc ctgggcctgc agcccaactt caagaccaac ttcgacctga
     1741 gcgaggacgc caagctgcag ttcagcaagg acacctacga ggaggacctg gagaacctgc
     1801 tggcccagat cggcgacgag tacgccgacc tgttcctggc cgccaagaac ctgtacgacg
     1861 ccatcctgct gagcggcatc ctgaccgtga ccgacaacag caccaaggcc cccctgagcg
     1921 ccagcatgat caagaggtac gaggagcacc agaaggacct ggcccagctg aagcagttca
     1981 tcaaggagca cctgcccgac aagtacaacg agatcttcag cgacaagagc aagaacggct
     2041 acgccggcta catcgagggc aagaccagcc aggaggactt ctacaagtac ctgaagaaga
     2101 tcctgagcaa gatcgacggc gccgagtact tcctggacaa gatcgacagg gaggacttcc
     2161 tgaggaagca gaggaccttc gacaacggca gcatccccca ccagatccac ctgcaggagc
     2221 tgcacgccat cctgaggagg cagggcgagt actacccctt cctgaaggag aaccaggaga
     2281 agatcgagaa gatcctgacc ttcaggatcc cctactacgt gggccccctg gccaggggca
     2341 acagcaggtt cgcctggctg accaggaaga gcgacgagaa gatcaccccc tggaacttcg
     2401 acgaggtggt ggacaaggag agcagcgccg aggccttcat cgagaggatg accaactacg
     2461 acacctacct gcccaacgag aaggtgctgc ccaagcacag cctgctgtac gagaccttca
     2521 ccgtgtacaa cgagctgacc aaggtgaagt acgtgaccga gaggggcaag acccagcagt
     2581 tcttcagcgc cgagcagaag caggagatct tcgaccacct gttcaagaag aacaggaagg
     2641 tgaccaagaa gaagctgaag gactacctgg agaaggagtt cgaggagttc gacagcgtgg
     2701 acatcaccgg cgtggaggac gagttcaacg ccagcctggg cacctaccac gacctgctga
     2761 agatcctgaa ggacaaggac ttcctggacg acgaggagaa cgaggagatc ctggaggaca
     2821 tcgtgctgac cctgaccctg ttcgaggaca gggagatgat caggaagagg ctggagaagt
     2881 acagcgacct gttcaccaag gagcagctga agaagctgga gaggaggcac tacaccggct
     2941 ggggcaggct gagcaggaag ctgatcaacg gcatcaggga caagcagagc ggcaagacca
     3001 tcctggacta cctgatcgac gacggcagca gcaacaggaa cttcatgcag ctgatcaacg
     3061 acgacagcct gagcttcaag gaggagatcg agaaggccca ggtgatcggc gagaccgaca
     3121 acctgcacga ggtggtgcag gacctggccg gcagccccgc catcaagaag ggcatcctgc
     3181 agagcctgaa gatcgtggac gagctggtga aggtgatggg caggcacaac cccgagaaca
     3241 tcgtggtgga gatggccagg gagaaccaga ccaccaacag gggcaggagg aacagcaggc
     3301 agaggctgaa gaggctggag gaggccctga agaacctggg cagcaacatc ctgaaggagc
     3361 accccgtgga caaccagcag ctgcagaacg acaggctgta cctgtactac ctgcagaacg
     3421 gcaaggacat gtacaccggc gaggagctgg acatcgacaa cctgagccag tacgacatcg
     3481 accacatcat cccccagagc ttcatcaagg acaacagcat cgacaacagg gtgctgacca
     3541 gcagcgagga gaacaggggc aagagcgaca acgtgcccag catcgaggtg gtgaggaaga
     3601 tgaagagctt ctggcagaag ctgctgaacg ccggcctgat cagccagagg aagttcgaca
     3661 acctgaccaa ggccgagagg ggcggcctga ccgaggacga caaggccggc ttcatcaaga
     3721 ggcagctggt ggagaccagg cagatcacca agcacgtggc ccagatcctg gacagcaggt
     3781 tcaacaccga gagggacgag aacgacaaga ggatcaggaa cgtgaagatc atcaccctga
     3841 agagcaacct ggtgagccag ttcaggaagg acttcggcct gtacaaggtg agggagatca
     3901 acgactacca ccacgcccac gacgcctacc tgaacgccgt ggtgggcacc gccctgctga
     3961 agaagtaccc caagctggag cccgagttcg tgtacggcga ctacaagaag tacaacctga
     4021 ggaagctgat cgccaagagc gacagggaga ggggcaaggc caccgccaag atgttcttct
     4081 acagcaacat catgaacttc ttcaagaccg aggtgaagct ggccgacggc accgtgatcg
     4141 agaggcccgt gatcgagacc aacaaggaga ccggcgagat cgtgtgggac aaggagaagg
     4201 acttcgccac cgtgaggaag gtgctgagct acccccaggt gaacatcgtg aagaaggtgg
     4261 aggtgcagac cggcggcttc agcaaggaga gcatcctgcc caagggcaac agcgacaagc
     4321 tgatccccag gaagaacaac tgggacccca agaagtacgg cggcttcgac agccccaccg
     4381 tggcctacag cgtgctggtg gtggccgacg tggagaaggg caaggccaag aagctgaaga
     4441 ccgtgaagga gctggtgggc atcaccatca tggagaggag cgccttcgag aagaacccca
     4501 tcgccttcct ggagaagaag ggctaccaga acatccagga ggacctgatc atcaagctgc
     4561 ccaagtacag cctgttcgag ctggagaacg gcaggaggag gctgctggcc agcgccaagg
     4621 agctgcagaa gggcaacgag atggtgctgc ccgcccacct ggtgaccctg ctgtaccacg
     4681 ccaagaggat cgacaagagc aacggcaaca gcgagaacct ggagcagaag caggagtacg
     4741 tggagaagca caagaacgag ttcgacgagc tgctggacta catcatcgac ttcagcgaga
     4801 agtacatcct ggccgacaag aacctggaga agatcaagga gctgtacgac cagaacgacg
     4861 acgccgacat caacgagctg gccagcagct tcatcaacct gctgaccttc accgccctgg
     4921 gcgcccccgc cgccttcaag ttcttcggcg agaccatcga caggaagagg tacaccagca
     4981 ccaaggaggt gctgaacgcc accctgatcc accagagcat caccggcctg tacgagacca
     5041 ggatcgacct gagcaagctg ggcgaggacc accatcatca tcaccacgcc tctggaacag
     5101 gcggacctaa aaagaagagg aaggtttaag gtaccgagct cggatccact agtccagtgt
     5161 ggtggaattc tgcagatatc cagcacagtg gcggccgctc gagtctagag ggcccgttta
     5221 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     5281 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     5341 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     5401 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     5461 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
     5521 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     5581 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     5641 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     5701 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     5761 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     5821 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     5881 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
     5941 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     6001 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     6061 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     6121 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     6181 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
     6241 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
     6301 atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
     6361 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
     6421 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
     6481 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
     6541 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
     6601 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
     6661 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
     6721 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
     6781 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
     6841 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc
     6901 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     6961 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     7021 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     7081 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     7141 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
     7201 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     7261 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     7321 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     7381 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     7441 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     7501 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     7561 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     7621 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     7681 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     7741 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     7801 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     7861 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     7921 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     7981 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     8041 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     8101 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     8161 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     8221 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     8281 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     8341 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     8401 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     8461 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     8521 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     8581 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     8641 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     8701 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     8761 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     8821 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     8881 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     8941 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     9001 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     9061 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     9121 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     9181 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     9241 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     9301 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     9361 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     9421 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     9481 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     9541 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     9601 taggggttcc gcgca
//
