LOCUS       sequence-363631-        9576 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMV and T7 promoter expression plasmid for human codon usage 
            anCas(PDCA) with an N-terminal NLS and C-terminal NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9576)
  AUTHORS   Alonso-Lerma B, Jabalera Y, Samperio S, Morin M, Fernandez A, Hille 
            LT, Silverstein RA, Quesada-Ganuza A, Reifs A, Fernandez-Penalver S,
            Benitez Y, Soletto L, Gavira JA, Diaz A, Vranken W, Sanchez-Mejias 
            A, Guell M, Mojica FJM, Kleinstiver BP, Moreno-Pelayo MA, Montoliu 
            L, Perez-Jimenez R
  TITLE     Evolution of CRISPR-associated endonucleases as inferred from 
            resurrected proteins.
  JOURNAL   Nat Microbiol. 2023 Jan;8(1):77-90. doi: 10.1038/s41564-022-01265-y.
            Epub 2023 Jan 2.
  PUBMED    36593295
REFERENCE   2  (bases 1 to 9576)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-022-01265-y"; journalName: "Nat Microbiol"; date: 
            "2023-01"; volume: "8"; issue: "1"; pages: "77-90"
COMMENT     Sequence Label: pCMV-T7-NLS(SV40)-anCas(PDCA)-6xHis-NLS(SV40)
FEATURES             Location/Qualifiers
     source          1..9576
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(55..74)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        246..625
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        626..829
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     780..800
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     874..893
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        874..892
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             943..963
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5074..5091
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5110..5130
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5178..5195)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5184..5408
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5454..5882
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5541..5560)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5751..5772
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5891..5911)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5896..6225
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6076..6211
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6138..6157
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6292..7086
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6346..6365)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6956..6975
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7260..7381
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7297..7316)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7351..7370
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7430..7446)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7430..7446)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7443..7465)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7454..7470
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7478..7484,7485..7502,7503..7508))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7523..7544
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7661..7678)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7832..8420)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7912..7931)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8591..9382,9383..9451))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9214..9233
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9452..9556)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat
       61 ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc
      121 tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat
      181 gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac
      241 gcgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
      301 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      361 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      421 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      481 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      541 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      601 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
      661 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
      721 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
      781 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac
      841 tagagaaccc actgcttact ggcttatcga aattaatacg actcactata gggagaccca
      901 agctggctag cgtttaaact taagcttggt accgccacca tgcccaagaa aaagcggaag
      961 gtgcccaaga tggacaagaa atactctatc ggtctggata tcggcaccaa cagcgtgggc
     1021 tgggccgtga tcacagacga ttacaaagtg cctagcaaaa agttcaaggt gctgggaaac
     1081 accgatagac acagcatcaa gaagaatttg atcggcgccc tgctgttcga tagcggcgaa
     1141 accgccgaag ccaccagact gaagaggacc gcccggagaa gatacaccag aagaaagaac
     1201 cgaatttgtt acctgcagga gatcttttct aatgagatgg ccaaggtgga cgactctttt
     1261 ttccacagac tggaagagtc cttcctggtg gaggaggaca agaagcacga gcggcacccc
     1321 atcttcggaa acatcgtgga cgaggtggcc tatcacgaga agtatcccac catctaccac
     1381 ctgagaaaga agctggccga ctccaccgac aaagcagacc tgagactgat ctatctggcc
     1441 ctggctcaca tgatcaaatt ccgcggccac ttcctgatcg agggagatct caaccctgat
     1501 aacagcgatg tggacaagct gttcatccag ctggtgcaga cctacaacca gctgttcgag
     1561 gagaatccca tcaacgcctc tggagtggac gccaaggcca tcctgagcgc cagactgagc
     1621 aagtcccgga gactggagaa cctgatcgcc cagctgcctg gcgagaagaa aaacggcctg
     1681 tttggaaatc tgatcgcact gtccctggga ctgacaccta atttcaagag caacttcgac
     1741 ctggccgagg atgctaaact gcagctgagc aaagacacct acgacgatga cctggacaac
     1801 ctgctggccc aaatcggcga ccagtacgcc gacctgttcc tggccgccaa gaacctgagc
     1861 gacgccattc tgctcagcga tatcctgaga gtgaacagcg agatcacaaa ggcacctctg
     1921 agcgctagca tgatcaagcg atacgatgag caccaccagg acctgaccct gctcaaggct
     1981 ctggtgcggc agcagctgcc agaaaaatac aaggagatct ttttcgacca gagcaagaat
     2041 ggatacgccg gctacatcga cggcggcgcc tctcaggagg agttctacaa gttcattaag
     2101 cctatcctgg agaagatgga cggcacagag gaactcctgg ccaaactcaa ccgggaggac
     2161 ctcctgagaa aacagcggac attcgacaat ggcagcatcc cccaccagat ccacctgggc
     2221 gagctgcacg ccatcctgag gaggcaggag gacttctacc cattcctgaa ggacaacaga
     2281 gagaagatcg agaagatcct gaccttcaga attccttact acgtgggacc tctggccaga
     2341 ggcaactcca gattcgcctg gatgacccgg aagagcgagg aaaccatcac cccttggaac
     2401 ttcgaggagg tggtggataa gggcgcttct gcccaaagct tcatcgaaag aatgacaaac
     2461 ttcgacaaga acctgccaaa cgaaaaggtg ctgcctaaac actctctgct gtacgagtac
     2521 tttaccgtgt acaacgagct gaccaaggtg aagtacgtta cagagggcat gagaaaacct
     2581 gccttcctaa gcggcgagca gaaaaaggct atcgtggatc tgctgtttaa gaccaaccgg
     2641 aaagtgaccg tgaagcagct gaaagaggac tacttcaaga agatcgagtg ctttgactct
     2701 gtggaaatat ctggcgtgga ggaccggttc aacgcaagcc tgggaacata ccacgacctg
     2761 ctgaagatca tcaaagacaa ggatttcctg gacaatgaag aaaacgagga tatcctggaa
     2821 gatattgtgc tgacactgac actgttcgag gacagagaga tgatcgaaga aagactgaaa
     2881 acgtacgccc atctgttcga tgataaagtg atgaagcagc tgaaaagacg gcactacacc
     2941 ggctggggca gactgtcccg gaagctgatc aacggcatcc gtgacaagca gagcggcaag
     3001 acaatcctgg actttctcaa gagcgacggc ttcgccaacc ggaactttat gcagctgatc
     3061 cacgacgact ccctgacatt caaggaggat atccaaaaag cccaggtgtc cggccagggc
     3121 gattccctcc atgagcagat agctaacctg gctggcagcc ccgccatcaa gaaaggcatc
     3181 ctccagaccg tcaaggtggt ggatgagctg gtgaaggtga tgggcagaca taagcccgag
     3241 aatatcgtta ttgagatggc tagagaaaac cagacaaccc agaaaggcca gaagaacagc
     3301 agagagcgga tgaaaagaat cgaggaaggc atcaaggaac tgggcagcca gatcctcaag
     3361 gaacaccctg tggaaaacac ccagctacaa aatgagaagc tgtacctgta ttacctgcaa
     3421 aacggccggg acatgtacgt ggaccaggag ctcgacatca accggctgag cgattatgac
     3481 gtggaccaca tcgtgcccca gtcattcatc aaagatgaca gcatcgacaa caaggtgctg
     3541 acccggagcg acaagaatag aggcaagtct gacaatgtgc ccagcgaaga agtggtgaaa
     3601 aagatgaaga attattggcg gcagctgctg aacgccaagc tgattacaca gcggaagttc
     3661 gataacctga caaaggccga gagaggaggc ctgtctgaac tggacaaggc cggcttcatc
     3721 aagcggcagc tggtggagac cagacagatc acaaagcacg tggctcaaat cctggacagc
     3781 agaatgaata ccaagtacga cgaaaacgac aagctaatcc gggaggtgaa ggtgatcacc
     3841 ctgaagtcaa agctggtttc cgacttcaga aaggatttcc agttctacaa ggtgcgggaa
     3901 atcaacaact accaccacgc ccacgacgcc tatctgaacg ctgtcgtcgg aaccgccctc
     3961 atcaagaagt accccaagct ggaatctgag ttcgtgtacg gcgattacaa ggtttacgac
     4021 gtgcgcaaga tgatcgccaa gagtgaacaa gaaatcggaa aagcgacagc caagagattc
     4081 ttctacagca atatcatgaa cttcttcaag accgagatca ccttagccaa tggagaaatc
     4141 agaaagcgcc ccctgatcga aactaacggc gagacaggcg agatcgtctg ggacaaaggc
     4201 agagattttg ccacagtcag aaaggtgctg agcatgccac aagtgaatat cgtgaagaag
     4261 accgaggtgc agaccggcgg atttagcaag gaaagcatcc tgcctaagag aaacagcgat
     4321 aagctgattg cccggaaaaa ggactgggat cctaaaaaat acgggggctt tgattcccct
     4381 accgtggcct atagcgtgct ggtggtggcc aaggtcgaaa agggcaaggc caagaagctg
     4441 aaatccgtga aagaattggt tggaattacc atcatggaac gcagcagctt cgagaagaac
     4501 cctatagact tcctggaagc taaagggtac aaggacgtac agaaagacct aatcatcaag
     4561 ctgccaaagt acagcctgtt cgagcttgag aacggcagaa gacggatgct ggccagcgcc
     4621 ggtgagttgc agaaaggtaa cgaaatggtg ctgcctgcca aactggtgac attcctttac
     4681 catgccagcc acatcgagaa gagcaagggc tctcctgaga acaacgagca aaagcaggcc
     4741 tacatcgagc aacacaagca cgacctggac gagatcctgg aatacatcag cgaattcagc
     4801 aagagataca tcctggccga taagaatcta tctaaggtga aatcactgtt taacaagcac
     4861 gaggatagca gcatcagcga gctggcaagc agcatcatca acctgttcac cctgacaagc
     4921 ctgggcgccc ctgctgcttt caagttcctg gataccacca tcgacagaaa aagatacacc
     4981 agcaccaagg aagtgctgga tgccaccctg atccaccagt caataacagg cctgtacgag
     5041 acacggatcg acctctctca gctgggcgga gatcaccatc atcatcacca cgcctctgga
     5101 acaggcggac ctaaaaagaa gaggaaggtt taagcggccg ctcgagtcta gagggcccgt
     5161 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     5221 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     5281 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     5341 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     5401 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     5461 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     5521 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     5581 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     5641 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     5701 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     5761 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     5821 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     5881 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     5941 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     6001 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     6061 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     6121 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
     6181 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
     6241 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa
     6301 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     6361 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     6421 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
     6481 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     6541 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     6601 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     6661 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     6721 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     6781 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
     6841 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     6901 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     6961 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     7021 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     7081 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     7141 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     7201 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     7261 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     7321 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     7381 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     7441 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     7501 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     7561 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     7621 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     7681 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     7741 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     7801 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     7861 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     7921 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     7981 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     8041 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     8101 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     8161 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     8221 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     8281 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     8341 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     8401 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     8461 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     8521 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     8581 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     8641 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     8701 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     8761 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     8821 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     8881 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     8941 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     9001 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     9061 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     9121 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     9181 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     9241 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     9301 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     9361 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     9421 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     9481 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     9541 aaataggggt tccgcgcaca tttccccgaa aagtgc
//
