LOCUS       sequence-36364-e         733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Zhu C, Smith T, McNulty J, Rayla AL, Lakshmanan A, Siekmann AF, 
            Buffardi M, Meng X, Shin J, Padmanabhan A, Cifuentes D, Giraldez AJ,
            Look AT, Epstein JA, Lawson ND, Wolfe SA
  TITLE     Evaluation and application of modularly assembled zinc-finger 
            nucleases in zebrafish.
  JOURNAL   Development. 2011 Oct;138(20):4555-64.
  PUBMED    21937602
REFERENCE   2  (bases 1 to 733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2011-10"; volume: "138(20)"; pages: "4555-64"
COMMENT     Sequence Label: pBS-F1-GTC
FEATURES             Location/Qualifiers
     source          1..733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(292..312)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(292..310)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(331..347)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(331..347)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(344..366)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    355..371
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(379..385,386..403,404..409))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    424..445
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(562..579)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cctgtcgagt cctgtgaccg ccgcttcagt gaccgttctg ctctcgctcg tcacatccgg
       61 atccacaccg gccagaagcc cttccagtgt cgcatctgca tgcgtaactt cagtcgctcc
      121 agccacctga ccacccacat caggacccac acaggcgaga agccttttgc atgcgacatt
      181 tgtgggagga agtttgccag gagtgatgaa cgcaagaggc ataccaaaat ccatacaggt
      241 gaaaagtaat ctagagcggc cgccaccgcg gtggagctcc agcttttgtt ccctttagtg
      301 agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
      361 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
      421 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
      481 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
      541 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
      601 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
      661 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
      721 gttgctggcg ttt
//
