LOCUS       sequence-363648-        4256 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Subcloning YAP1 S127A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Hatterschide J, Castagnino P, Kim HW, Sperry SM, Montone KT, Basu D,
            White EA
  TITLE     YAP1 activation by human papillomavirus E7 promotes basal cell 
            identity in squamous epithelia.
  JOURNAL   Elife. 2022 Feb 16;11. pii: 75466. doi: 10.7554/eLife.75466.
  PUBMED    35170430
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-02-16"; volume: "11. pii: 75466. doi"; pages: " 
            10.7554/eLife.75466"
COMMENT     Sequence Label: p8321 pDONR ATG-YAP1 S127A-Stop
FEATURES             Location/Qualifiers
     source          1..4256
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     693..709
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     693..709
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     714..733
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        714..732
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    750..849
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2316..2415)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2431..2448)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2431..2447)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2440..2462)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      2511..2597
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2689..2716
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(2875..2892)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3046..3634)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3126..3145)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3727..4256,1..262))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cttctacagc gcggagaatc tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt
       61 tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc agcaaatcaa
      121 tatcactgtg tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt acggccagca
      181 acgtcggttc gagatggcgc tcgatgacgc caactacctc tgatagttga gtcgatactt
      241 cggcgatcac cgcttccctc ataatgttta actttgtttt agggcgactg ccctgctgcg
      301 taacatcgtt gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct
      361 tggatgcccg aggcatagac tgtaccccaa aaaaacagtc ataacaagcc atgaaaaccg
      421 ccactgcgcc gttaccaccg ctgcgttcgg tcaaggttct ggaccagttg cgtgagcgca
      481 tacgctactt gcattacagc ttacgaaccg aacaggctta tgtccactgg gttcgtgcct
      541 tcatccgttt ccacggtgtg cgtcacccgg caaccttggg tagcagcgaa gtcgaggcat
      601 ttctgtcctg gctggtctag aggaggcatg atgatatatt tttatcttgt gcaatgtaac
      661 atcagagatt ttgagacacg ggccagagct gccaggaaac agctatgacc atgtaatacg
      721 actcactata ggggatatca gctggatggc aaataatgat tttattttga ctgatagtga
      781 cctgttcgtt gcaacaaatt gataagcaat gctttcttat aatgccaact ttgtacaaga
      841 aagttgggtt tataaccatg taagaaagct ttctttatct agcttggtgg cagccaaaac
      901 agactccatg tcattaagga tgtcagaact caaagcttcc tgcagacttg gcatcagctc
      961 ctctccttct atgttcattc catctccttc cagtgttcca aggtccacat ttgtcccagg
     1021 aatggcttca aggtagtctg ggaaacggtt ctgctgtgag ggcagggtgc tttggttgat
     1081 agtatcacct gtatccatct catccacact gttcaggaag tcatctgggg ttcgagggac
     1141 actgtagctg ctcatgctta gtccactgtc tgtactctca tctcgagagt gataggtgcc
     1201 actgttaagg aaaggatctg agctattggt cgtcattgtt ctcaattcct gagacatccc
     1261 gggagaagac actggatttt gagtcccacc atcctgctcc agtgttggta actggctacg
     1321 cagggctaac tcctgccgaa gcagttcttg ctgtttcagc cgcagcctct ccttctccat
     1381 ctgcagttgc tgcagtcgca tctgttgctg ctggttggag ttgctgccac ccatgacgcc
     1441 tccctgtggg ctctggggag ccaggggtgg tggctgtttc actggagcac tctgactgat
     1501 tctctggttc atggcaaaac gagggtcaag ccttgggtct agccaagagg tggtcttgtt
     1561 cttatggttt atatagtaaa tttctccatc ctgagtcatg gcttgttccc atccatcagg
     1621 aagaggacct gaagccgagt tcatcatatt ctgctgcact ggtggactgg tgggggctgt
     1681 gacgttcatc tgggacagca tggccttcct ggggtcctgc catgttgttg tctgatcgat
     1741 gtgatttaag aagtatctct gaccagaaga tgtctttgcc atctcccaac ctgctggcag
     1801 aggtacatca tcaggtatct caaaagaaga ctgtcgaaga tgctgagctg tgggtgtagc
     1861 tgctgggcca gagactactc cagtgggggt cagtgtccca ggagaaacag ctcccaactg
     1921 cagagaagct ggagaggcat gagctcgaac atgctgtgga gtcagggctc ctgcagtgcc
     1981 tgcatcagta ctggcctgtc gggagtggga tttgggctcc ggcggcttga agaaggagtc
     2041 gggcagcttc cggagcctca tgggcacggt ctggggcacg ttggccgtct tggggttcat
     2101 gacggcgttg aagagcgcct ccaggtcggt ctccgagtcc ccgcggacgt gcacgatctg
     2161 atgcccggcg gggggtgcct gcggcgccgc ctgggtcgcc gcgggtgccg gttgcccggg
     2221 tccggacggc gggccctgcc cctgcggggg ctgcgaaggc ggctgccctt ggccctgggg
     2281 ggccggttga ggcggcggct gctgcccggg atccatgcca acttttttgt acaaagttgg
     2341 cattataaaa aagcattgct catcaatttg ttgcaacgaa caggtcacta tcagtcaaaa
     2401 taaaatcatt atttggggcc cgagcttaag actggccgtc gttttacaac gtcgtgactg
     2461 ggaaaacatc catgctagcg ttaacgcgag agtagggaac tgccaggcat caaataaaac
     2521 gaaaggctca gtcggaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     2581 tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgtgaagcaa cggcccggag
     2641 ggtggcgggc aggacgcccg ccataaactg ccaggcatca aactaagcag aaggccatcc
     2701 tgacggatgg cctttttgcg tttctacaaa ctcttcctgg ctagcggtac gcgtattaat
     2761 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     2821 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     2881 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     2941 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     3001 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     3061 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     3121 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     3181 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     3241 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     3301 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     3361 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     3421 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     3481 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     3541 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     3601 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     3661 gtctgacgct cagtggaacg acgcgtaact cacgttaagg gattttggtc atgggtggct
     3721 cgagggttat ttgccgacta ccttggtgat ctcgcctttc acgtagtgga caaattcttc
     3781 caactgatct gcgcgcgagg ccaagcgatc ttcttcttgt ccaagataag cctgtctagc
     3841 ttcaagtatg acgggctgat actgggccgg caggcgctcc attgcccagt cggcagcgac
     3901 atccttcggc gcgattttgc cggttactgc gctgtaccaa atgcgggaca acgtaagcac
     3961 tacatttcgc tcatcgccag cccagtcggg cggcgagttc catagcgtta aggtttcatt
     4021 tagcgcctca aatagatcct gttcaggaac cggatcaaag agttcctccg ccgctggacc
     4081 taccaaggca acgctatgtt ctcttgcttt tgtcagcaag atagccagat caatgtcgat
     4141 cgtggctggc tcgaagatac ctgcaagaat gtcattgcgc tgccattctc caaattgcag
     4201 ttcgcgctta gctggataac gccacggaat gatgtcgtcg tgcacaacaa tggtga
//
