LOCUS       sequence-36365-e         732 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Zhu C, Smith T, McNulty J, Rayla AL, Lakshmanan A, Siekmann AF, 
            Buffardi M, Meng X, Shin J, Padmanabhan A, Cifuentes D, Giraldez AJ,
            Look AT, Epstein JA, Lawson ND, Wolfe SA
  TITLE     Evaluation and application of modularly assembled zinc-finger 
            nucleases in zebrafish.
  JOURNAL   Development. 2011 Oct;138(20):4555-64.
  PUBMED    21937602
REFERENCE   2  (bases 1 to 732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2011-10"; volume: "138(20)"; pages: "4555-64"
COMMENT     Sequence Label: pCS2-F1-GTG-F2-GGT-F3-GAA
FEATURES             Location/Qualifiers
     source          1..732
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             19..39
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             40..129
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
ORIGIN
        1 ggatctgcca ccatggctcc aaagaagaag cgtaaggtat acccatacga tgttcctgac
       61 tatgcgggct atccctatga cgtcccggac tatgcaggat cgtatccata tgacgttcca
      121 gattacgctg ctcatggtac ccgcccatat gcttgccctg tcgagtcctg tgaccgccgc
      181 ttcagccgta gcgacgcgct cacccgccat atccggattc acacaggcca gaagcccttc
      241 cagtgtcgca tctgcatgag gaacttcagc tgtgcgcatc acctcacccg ccacatccgc
      301 acccacacag gcgagaagcc ttttgcatgc gacatctgcg gcaggaagtt cgcgcagttg
      361 tctaacctca cccgccatac caaaatccat acaggtggat cccaactagt caaaagtgaa
      421 ctggaggaga agaaatctga acttcgtcat aaattgaaat atgtgcctca tgaatatatt
      481 gaattaattg aaattgccag aaattccact caggatagaa ttcttgaaat gaaggtaatg
      541 gaatttttta tgaaagttta tggatataga ggtaaacatt tgggtggatc aaggaaaccg
      601 gacggagcaa tttatactgt cggatctcct attgattacg gtgtgatcgt ggatactaaa
      661 gcttatagcg gaggttataa tctgccaatt ggccaagcag atgaaatgca acgatatgtc
      721 aaagaaaatc aa
//
