LOCUS       sequence-36369-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  TITLE     2-series LIC N-terminal fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET His6 GST TEV LIC cloning vector (2G-T)
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(101..121)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(131..784)
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     complement(151..173)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             complement(794..811)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatcagcgc gcacgcgtgg taccggccgg ccaccaggtg
       61 gcgcgccgcg atcgcggatc cgttatccac ttccaatatt ggattggaag tacaggtttt
      121 cctcgatccc ttttggagga tggtcgccac caccaaacgt ggcttgccag ccctgcaaag
      181 gccatgctat atacttgctg gatttcaagt acttatcaat ttgtgggata gcttcaatac
      241 gttttttaaa acaaactaat tttgggaacg catccaggca cattgggtcc atgtataaaa
      301 caacatcaag agcgtcatac aacatgaagt caggatgggt tacatgatca ccatttaaat
      361 atgttttatg acataaacga tcttcgaaca ttttcagcat ttcaggtagc ttgctaagaa
      421 aatcaacttt gagagtttca aagtctttac tatatgcaat tctcgaaaca ccgtatctaa
      481 tatccaaaac cgctccttca agcattgaaa tctctgcacg ctcttttgga caaccaccca
      541 acatgttgtg cttgtcagct atataacgta tgatggccat agactgtgtt aatttaacat
      601 caccatcaat ataataagga agattgggaa actccaaacc caattcaaac tttttgtttc
      661 gccatttatc accttcatcg cgctcataca aatgctcttc atatttttct tcaagatatt
      721 ccaaaagaag tcgagtgggt tgcacaaggc ccttaatttt ccaataacct agtatagggg
      781 acatagaaga accatggtga tggtgatggt gagaagattt catatgtata tctccttctt
      841 aaagttaaa
//
